Transglycosylation properties of a Kluyveromyces lactis enzyme preparation: Production of tyrosol β-fructoside using free and immobilized enzyme

Hollá, Veronika; Karkeszová, Klaudia; Antošová, Monika; Polakovič, Milan

doi:10.1016/j.procbio.2021.08.016

Cited by 7 publications

(3 citation statements)

References 43 publications

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“…Moreover, many microeukaryotic genera were positively related to the development of terpenoids and flavonoids in icewine. It is due to these compounds commonly present in glycosidic bound forms, while the epiphytic microeukaryotes of grapes can generate a series of glycoside hydrolases (GHs) and glycosyl transferases (GTs), which efficiently break glycosidic bonds and release the aglycones [2,31]. For instance, monoterpenyl disaccharide glycosides originated from plants and microbes, such as α-arabinofuranosidase, β-glycosidase, and β-galactosidase, hydrolyze glycosidic bound terpenes into free forms [32].…”

Section: Discussionmentioning

confidence: 99%

Characteristic metabolites drive the self-assembly of microeukaryotic communities during spontaneous fermentation of icewine

Chen

2023

New Advances in Saccharomyces

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Wine can be regarded as a nutritional source for the human diet. It contains many nutrients such as vitamins and minerals, organic acids, flavonoids, and terpenoids. The varietal aroma of wines originated from the symbionts of the grapes and epiphytic microbiota, which combinedly grew from the sexual reproduction of the plant through seeds or during clonal reproduction. Nowadays, more and more studies focus on the assembly process of epiphytic microbiota and design a synthetic microbial community based on regional characteristics to improve wine quality and biofunctions. This review synthesizes the current concepts on the construction of synthetic microbiota, analyzes the advantages and difficulties in designing a functional yeast community, and lists the practical tools for data processing and model construction. A well-designed yeast community will possess high robustness against environment interference, higher efficiency of fermentation, and higher yield of targeted bioproducts.

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Section: Discussionmentioning

confidence: 99%

Characteristic metabolites drive the self-assembly of microeukaryotic communities during spontaneous fermentation of icewine

Chen

2023

New Advances in Saccharomyces

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“…To overcome the many drawbacks of free lipase, enzyme immobilization techniques have been investigated. In comparison to the free enzyme, the immobilized enzyme has several advantages, such as easier separation and recycling, higher activity, more stable enzymes, more resistance to various environmental changes, non-toxicity and biocompatibility, or coupling with enzyme purification, making it suitable for more complex reaction environments and a variety of reactor configurations [ 3 , 10 , 11 , 12 ]. However, some lipases react with hydrophobic matrixes or other lipase molecules during the immobilization process, resulting in lipase inactivation.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Enzymatic Performance of Immobilized Pseudomonas fluorescens Lipase on ZIF-8@ZIF-67 and Its Application to the Synthesis of Neryl Acetate with Transesterification Reaction

Wang,

Xiong,

et al. 2024

Molecules

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In this study, hybrid skeleton material ZIF-8@ZIF-67 was synthesized by the epitaxial growth method and then was utilized as a carrier for encapsulating Pseudomonas fluorescens lipase (PFL) through the co-precipitation method, resulting in the preparation of immobilized lipase (PFL@ZIF-8@ZIF-67). Subsequently, it was further treated with glutaraldehyde to improve protein immobilization yield. Under optimal immobilization conditions, the specific hydrolytic activity of PFL@ZIF-8@ZIF-67 was 20.4 times higher than that of the free PFL. The prepared biocatalyst was characterized and analyzed by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared (FT-IR). Additionally, the thermal stability of PFL@ZIF-8@ZIF-67 at 50 °C was significantly improved compared to the free PFL. After 7 weeks at room temperature, PFL@ZIF-8@ZIF-67 retained 78% of the transesterification activity, while the free enzyme was only 29%. Finally, PFL@ZIF-8@ZIF-67 was applied to the neryl acetate preparation in a solvent-free system, and the yield of neryl acetate reached 99% after 3 h of reaction. After 10 repetitions, the yields of neryl acetate catalyzed by PFL@ZIF-8@ZIF-67 and the free PFL were 80% and 43%, respectively.

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“…The latter ones facilitate the biocatalytic process by excluding the tedious cultivation and purification step to obtain FTases with sufficient transfructosylation activity [14,15]. Many commercial enzyme preparations (pectinases, polygalacturonases, and glucanases) also have the potential to produce FOS because of their FTase side activity [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Production of Fructooligosaccharides Using a Commercial Heterologously Expressed Aspergillus sp. Fructosyltransferase

Karkeszová

Polakovič

2023

Catalysts

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The catalytic properties of Seqenzym® FT, a fungal fructosyltransferase heterologously expressed in yeasts, were investigated at a temperature of 55 °C and pH 5.5. The initial rate measurements showed that the transfructosylation rate was only slightly inhibited by sucrose above the concentration of 1.5 M. A rather low level of hydrolytic side activity was observed even at sucrose concentrations as low as 0.25 M. In progress curve experiments, the mass yield of fructooligosaccharides (FOS) reached a maximum value of 57% at this sucrose concentration, although it dropped to about 35% later on. At high initial sucrose concentrations up to 2 M, the FOS yield reached a maximum value of approximately 63% at a sucrose conversion of approximately 90%. Although neither the yield nor the conversion changed much later on, the progress of the reaction was manifested by the gradual depletion of shorter chain FOS, 1-kestose and nystose, and the accumulation of 1-β-fructofuranosyl nystose. At initial sucrose concentrations of 2 M, the degree of polymerization expressed through the number of fructosyl units grew from 2.3 at a conversion degree of 87% to 3.1 at a conversion degree of 94%. Compared to other commercial preparations, Seqenzym® FT can better produce FOS with a higher degree of polymerization.

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Transglycosylation properties of a Kluyveromyces lactis enzyme preparation: Production of tyrosol β-fructoside using free and immobilized enzyme

Cited by 7 publications

References 43 publications

Characteristic metabolites drive the self-assembly of microeukaryotic communities during spontaneous fermentation of icewine

Characteristic metabolites drive the self-assembly of microeukaryotic communities during spontaneous fermentation of icewine

Enhanced Enzymatic Performance of Immobilized Pseudomonas fluorescens Lipase on ZIF-8@ZIF-67 and Its Application to the Synthesis of Neryl Acetate with Transesterification Reaction

Production of Fructooligosaccharides Using a Commercial Heterologously Expressed Aspergillus sp. Fructosyltransferase

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