Transient Accumulation of Nitrite Reductase mRNA in Maize following the Addition of Nitrate

Kramer, Vance C.; Lahners, Kristine N.; Back, Eduard; Privalle, Laura; Rothstein, Steven J.

doi:10.1104/pp.90.3.1214

Cited by 46 publications

(35 citation statements)

References 15 publications

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“…The mRNA for NiR was induced approximately 100-fold by the same treatment and followed the same pattern in that both levels peaked 2 h after the addition of nitrate and decreased thereafter. The transient nature of this induction, although quite similar to that reported for roots of maize seedlings treated with 20 mm nitrate (14), is in contrast to that found in soybean (8), spinach (18), or morning glory (28) cell suspensions. The age of our BMS cultures affects the degree of induction but not the transient nature of the phenomenon (data not shown).…”

Section: Induction Of Nir Mrna By Nitratecontrasting

confidence: 46%

“…Furthermore, similar experiments had been done using maize seedlings monitoring NiR mRNA, and transient induction was observed (14). Therefore, to monitor the effects of nitrate at the transcriptional level in BMS cell suspensions, the kinetics of induction of the message for NiR was monitored concurrently with NR activity even though these two may be unrelated.…”

Section: Induction Of Nir Mrna By Nitratementioning

confidence: 99%

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Nitrate Effects on Nitrate Reductase Activity and Nitrite Reductase mRNA Levels in Maize Suspension Cultures

Privalle

Lahners²,

Mullins³

et al. 1989

Plant Physiol.

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Nitrate reductase (NR) activity and nitrite reductase (NiR) mRNA levels were monitored in Black Mexican Sweet maize (Zea mays L.) suspension cultures after the addition of nitrate. Maximal induction occurred with 20 millimolar nitrate and within 2 hours. Both NR and NiR mRNA were transiently induced with levels decreasing after the 2 hours despite the continued presence of nitrate in the medium. Neither ammonia nor chlorate prevented the induction of NR. Furthermore, removal of nitrate, followed by its readdition 22 to 48 hours later, did not result in reinduction of activity or message. NR was synthesized de novo, since cycloheximide completely blocked its induction. Cycloheximide had no effect on the induction of NiR mRNA or on the transient nature of its induction. These results are similar to those reported previously for maize seedlings.

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Section: Induction Of Nir Mrna By Nitratecontrasting

confidence: 46%

Section: Induction Of Nir Mrna By Nitratementioning

confidence: 99%

Nitrate Effects on Nitrate Reductase Activity and Nitrite Reductase mRNA Levels in Maize Suspension Cultures

Privalle

Lahners²,

Mullins³

et al. 1989

Plant Physiol.

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“…Expression of NiR is not repressed by ammonium in leaves or roots from birch as was shown for maize (16). Hence, the faster NiR induction in birch leaves, which is opposite to the situation in maize, cannot be due to an inhibitory effect of ammonium in roots.…”

Section: Induction Of Nirmentioning

confidence: 74%

“…In contrast with barley (18), NR in birch is induced significantly faster in leaves than in roots. This is probably due to an inhibitory effect of ammonium on NR induction detectable only in roots (Table I) Accumulations of NiR-encoding mRNAs following the addition of nitrate have been observed in spinach (1,24), maize (16), and pea (12). Induction of f-glucuronidase mRNA by nitrate in a transgenic tobacco plant line harboring a spinach NiR promotor-f-glucuronidase construct was also reported (2).…”

Section: Induction Of Nirmentioning

confidence: 96%

Induction of Nitrate Assimilatory Enzymes in the Tree Betula pendula

Friemann

Lange²,

Hachtel³

et al. 1992

Plant Physiol.

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“…To take up various concentrations of nitrate, plants have two types of nitrate transporters, which are generally classified into low affinity transporters that are functional under the nitrate abundant condition (more than 1 mM nitrate), and high affinity transporters that take up nitrate under the nitrate-limiting condition (less than 1 mM nitrate) (Miller et al 2007). Furthermore, in agreement with the ability for taking up various concentrations of nitrate, nitrate response is known to be induced by a broad range of concentrations of nitrate (10 µM to 10 mM) in a dosage-dependent manner (Kramer et al 1989;Gowri et al 1992;Ho et al 2009;Hu et al 2009). …”

Section: Introductionmentioning

confidence: 87%

An NLP-binding site in the 3' flanking region of the nitrate reductase gene confers nitrate-inducible expression inArabidopsis thaliana(L.) Heynh.

Konishi

Yanagisawa

2013

Soil Science and Plant Nutrition

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As a signaling molecule, nitrate induces the expression of multiple genes involved in nitrate assimilation in plants. We previously showed that the expression of the Arabidopsis thaliana (L.) Heynh. nitrite reductase gene (NIR1) was activated by nitrate signaling through an interaction between NODULE INCEPETIONlike protein (NLP) transcription factors (NLP1-9) and a nitrate-responsive cis-element (NRE) in the NIR1 promoter. By in vitro binding assays, we have also demonstrated the binding of NLP6 to an NRE-like sequence, designated NIA1-NRE1, in the 3' flanking region of an A. thaliana nitrate reductase gene, NIA1.In our current study, we characterize the role of NIA1-NRE1 in NIA1 expression. Deletion analysis of the 3' flanking region of NIA1 revealed that a 345-bp sequence containing NIA1-NRE1 has a dominant role in nitrate-inducible NIA1 expression. We also found that the 35S minimal promoter fused to the 345-bp sequence, or to NIA1-NRE1, can confer nitrate-inducible expression. Furthermore, the NIA1-NRE1-fused 35S minimal promoter is transactivated by NLP6 in vivo. These results suggest that NIA1-NRE1 alone is sufficient to direct nitrate-responsive gene expression and that nitrate-inducible NIA1 expression is regulated through the interaction between NLPs and NIA1-NRE1. Additionally, we show that the distance between the NRE and the gene under the control of the NRE is not critical for this process.

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Transient Accumulation of Nitrite Reductase mRNA in Maize following the Addition of Nitrate

Cited by 46 publications

References 15 publications

Nitrate Effects on Nitrate Reductase Activity and Nitrite Reductase mRNA Levels in Maize Suspension Cultures

Nitrate Effects on Nitrate Reductase Activity and Nitrite Reductase mRNA Levels in Maize Suspension Cultures

Induction of Nitrate Assimilatory Enzymes in the Tree Betula pendula

An NLP-binding site in the 3' flanking region of the nitrate reductase gene confers nitrate-inducible expression inArabidopsis thaliana(L.) Heynh.

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