Transient, afferent input-dependent, postnatal niche for neural progenitor cells in the cochlear nucleus

Volkenstein, Stefan; Oshima, Koichiro; Sinkkonen, Saku T.; Corrales, C. Eduardo; Most, Sam P.; Chai, Renjie; Jan, Taha A.; Amerongen, Renée van; Cheng, Alan G.; Heller, Stefan

doi:10.1073/pnas.1307376110

Cited by 14 publications

(13 citation statements)

References 44 publications

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“…Axin2 expression reflects Wnt/β-catenin signaling activation and is a useful marker for functional stem cells in a variety of tissues (17)(18)(19)(20)(21). In Axin2-LacZ reporter mice, we observed LacZ signals in a subset of the cells near the basement membrane, but not in postmeiotic germ cells residing in the adluminal compartment of the seminiferous epithelium ( Fig.…”

Section: Resultsmentioning

confidence: 86%

Paracrine Wnt/β-catenin signaling mediates proliferation of undifferentiated spermatogonia in the adult mouse testis

Takase

Nusse

2016

Proc. Natl. Acad. Sci. U.S.A.

124

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Spermatogonial stem cells (SSCs) fuel the production of male germ cells but the mechanisms behind SSC self-renewal, proliferation, and differentiation are still poorly understood. Using the Wnt target gene Axin2 and genetic lineage-tracing experiments, we found that undifferentiated spermatogonia, comprising SSCs and transit amplifying progenitor cells, respond to Wnt/β-catenin signals. Genetic elimination of β-catenin indicates that Wnt/β-catenin signaling promotes the proliferation of these cells. Signaling is likely initiated by Wnt6, which is uniquely expressed by neighboring Sertoli cells, the only somatic cells in the seminiferous tubule that support germ cells and act as a niche for SSCs. Therefore, unlike other stem cell systems where Wnt/β-catenin signaling is implicated in self-renewal, the Wnt pathway in the testis specifically contributes to the proliferation of SSCs and progenitor cells.

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Section: Resultsmentioning

confidence: 86%

Paracrine Wnt/β-catenin signaling mediates proliferation of undifferentiated spermatogonia in the adult mouse testis

Takase

Nusse

2016

Proc. Natl. Acad. Sci. U.S.A.

124

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“…1 B-D) prompted us to ask if the Axin2-expressing population contains osteolineage progenitors. Hence, we performed fate mapping of the Axin2-expressing cells using the Axin2CreERT2 (AXCT2) mouse, which has been used to identify Wnt-responsive stem and progenitor cells in multiple organs including the mammary gland, brain, inner ear, and skin (47)(48)(49)(50). We pulsed AXCT2;Ai6 (ZsGreen reporter) mice (47,51) with tamoxifen at postnatal day 0 (P0), and allowed them to age to various time points for up to a year (Fig.…”

Section: Neonatal Wnt-responding Stromal Population Contains Progenitorsmentioning

confidence: 99%

Wnts produced by Osterix-expressing osteolineage cells regulate their proliferation and differentiation

Tan

Senarath-Yapa

Chung

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Wnt signaling is a critical regulator of bone development, but the identity and role of the Wnt-producing cells are still unclear. We addressed these questions through in situ hybridization, lineage tracing, and genetic experiments. First, we surveyed the expression of all 19 Wnt genes and Wnt target gene Axin2 in the neonatal mouse bone by in situ hybridization, and demonstrated-to our knowledge for the first time-that Osterix-expressing cells coexpress Wnt and Axin2. To track the behavior and cell fate of Axin2-expressing osteolineage cells, we performed lineage tracing and showed that they sustain bone formation over the long term. Finally, to examine the role of Wnts produced by Osterix-expressing cells, we inhibited Wnt secretion in vivo, and observed inappropriate differentiation, impaired proliferation, and diminished Wnt signaling response. Therefore, Osterix-expressing cells produce their own Wnts that in turn induce Wnt signaling response, thereby regulating their proliferation and differentiation.Wnt | bone | development | Osterix | Wntless

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“…Hearing loss results in a degeneration of cochlear nucleus neurons [11]. Interestingly, neuroplasticity is evident in the cochlear nucleus postnatally, which could be shown by the persistence of neuronal stem cells until adulthood [15] and the regulation of stem cells by changes in activation induced by cochlear ablation [16]. Further investigations have shown dependence of cell growth and neuronal survival on extracellular components such as laminin and poly-Dlysine [13,14].…”

Section: Introductionmentioning

confidence: 99%

Effects of the neurotrophic factors BDNF, NT-3, and FGF2 on dissociated neurons of the cochlear nucleus

Rak¹,

Völker²,

Frenz³

et al. 2014

NeuroReport

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The cochlear nucleus is the first relay station for acoustic information in the auditory pathway and its cellular integrity is affected by hearing loss. Neurotrophic factors, which are known to regulate fundamental processes in the brain, are expressed in the cochlear nucleus and are regulated by the changes in the stimulation. The aim of this study was to evaluate the effect of the neurotrophins Brain derived neurotrophic factor (BDNF) and Neurotrophin 3 (NT-3) and the neurotrophic factor Fibroblast growth factor 2 (FGF2) on primary cultured cells of the mouse cochlear nucleus. No effect on overall cell growth was detected after 8 days in culture by the factors applied. NT-3 had a strong impact on enhancement of neuronal survival, whereas BDNF stimulated neuronal survival and axonal outgrowth. Axonal branching was negatively affected by the administration of BDNF. FGF2 did not show any effect. The results presented represent fundamental research on auditory neurons, but might be one step toward defining novel therapeutic strategies in the future to prevent cochlear nucleus degeneration induced by hearing loss.

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Transient, afferent input-dependent, postnatal niche for neural progenitor cells in the cochlear nucleus

Cited by 14 publications

References 44 publications

Paracrine Wnt/β-catenin signaling mediates proliferation of undifferentiated spermatogonia in the adult mouse testis

Paracrine Wnt/β-catenin signaling mediates proliferation of undifferentiated spermatogonia in the adult mouse testis

Wnts produced by Osterix-expressing osteolineage cells regulate their proliferation and differentiation

Effects of the neurotrophic factors BDNF, NT-3, and FGF2 on dissociated neurons of the cochlear nucleus

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