Transient effect of weak electromagnetic fields on calcium ion concentration in Arabidopsis thaliana

Pazur, Alexander; Rassadina, V. V.

doi:10.1186/1471-2229-9-47

Cited by 37 publications

(28 citation statements)

References 36 publications

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“…Elevated concentrations of free cytosolic calcium are induced in response to various stimuli, such as red light, mechanical stimulation, cold shock, gravity, pathogen attack, phytohormones, drought and salinity (Toyota et al, 2008;Song et al, 2009). The monitoring of the bioluminescence of aequorin in Arabidopsis thaliana (Pazur and Rassadina, 2009) showed that combinations of magnetic and electromagnetic fields caused temporary changes in the internal Ca 2+ concentrations and that this phenomenon can be explained according to ion cyclotron resonance. However, the decreased total calcium content of the MF-treated maize plants in the present study is better explained according to the beneficial effects of the combination of the GeoMF and WEMF on the membrane integrity (the crucial sites of channels and pumps) or by the direct effect on the calcium channels and pumps.…”

Section: Resultsmentioning

confidence: 98%

Growth promotion and a decrease of oxidative stress in maize seedlings by a combination of geomagnetic and weak electromagnetic fields

Hajnorouzi¹,

Vaezzadeh²,

Ghanati³

et al. 2011

Journal of Plant Physiology

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Section: Resultsmentioning

confidence: 98%

Growth promotion and a decrease of oxidative stress in maize seedlings by a combination of geomagnetic and weak electromagnetic fields

Hajnorouzi¹,

Vaezzadeh²,

Ghanati³

et al. 2011

Journal of Plant Physiology

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“…Microwave cell exposure induces an increase in intracellular calcium concentration (Pazur and Rassadina, 2009). An intranuclear increase of calcium concentration has been shown to induce chromatin condensation (Martin and Cardoso, 2010).…”

Section: Discussionmentioning

confidence: 99%

Cell nucleus and membrane recovery after exposure to microwaves

Shckorbatov¹,

Pasiuga²,

Kolchigin³

et al. 2011

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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Communicated by Aleksandrs RapoportsCells of human buccal epithelium of six male donors were exposed to microwave radiation (frequency f = 36.64 GHz, power density W = 0.1, 1 and 4 W/m 2 ). Exposure time was 10 seconds. The state of chromatin in cell nucleus was estimated by a number of heterochromatin granules after staining with 2% orcein in 45 % acetic acid. Permeability of cell membranes was estimated by percentage of unstained cells after 5 min of staining the cells with vital dyes trypan blue (0.5%) and indigocarmine (5 mM). Cell exposure to microwaves induced chromatin condensation (increase of the number of heterochromatin granules) and increase of membrane permeability to trypan blue and indigocarmine. Isolated human buccal cells demonstrated the ability to recover after microwave exposure. The number of heterochromatin granules decreased to its initial level after 0.5 hour (W = 0.1 W/m2 ) and 2 hours (W = 1 and 4 W/m 2 ) after cell exposure. Cell plasma membrane permeability recovered later -after 1 hour and 3 hours post exposure, respectively. Key words: human cell, chromatin, heterochromatin, buccal epithelium, electromagnetic radiation exposure. 13Proc. Latvian Acad. Sci., Section B, Vol. 65 (2011), No. 1/2. after electromagnetic cell exposure has been shown earlier (Shckorbatov et al., 2009a;Pasiuga et al., 2009), but in this paper for the first time we test the dependence of cell recovery on the intensity of microwave exposure. MATERIALS AND METHODSHuman cells. The studies were conducted using buccal epithelium human cells. The cells were obtained from the inner surface of donor's cheek by light scraping with a blunt sterile spatula. This operation was absolutely bloodless and painless. All the donors were informed about the purpose of investigation. Our investigations were performed in accordance with the European Convention on Human Rights and Biomedicine (1997), Declarations and Recommendations of the First, the Second and the Third National (Ukrainian) Congresses of Bioethics (Kiev, Ukraine, 2001, 2004, 2007 and Ukrainian legislation.The cells were placed in a 3.03 mM phosphate buffer (pH = 7.0) and 2.89 mM calcium chloride (Reachem, Moscow, Russia) solution, and stored for further experiments. 25 µl of cell suspension containing several thousand of cells were placed on a glass slide and subjected to microwave exposure. Cells were stained immediately after the exposure, 30 minutes, 1 hour, 2 and 3 hours after cell exposure with 2% orcein (Merck AG, Darmstadt, Germany) solution in 45 % acetic acid (Reachem, Moscow, Russia), 5 mM indigocarmine (Merck AG, Darmstadt, Germany) and 0.5% trypan blue (Reachem, Moscow, Russia) solutions. All cell donors were men, non-smokers. Ages of the donors A to F, respectively, were 18, 20, 24, 35, 53, and 55 years. The male donors were selected by the reasons of convenience, as in our previous experiments no gender-related differences between buccal epithelium cells in charateristics of chromatin condensation or membrane permeability was observed. For light mic...

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“…The calcium concentration increases in cell under the action of EMFs [102,103]. During the chronic exposure the calcium ions are released from the membrane, which are membrane bound and are released into the cytosol [104].…”

Section: The Action Of Emf On Intracellular Regulatory Systemsmentioning

confidence: 99%

The Main Approaches of Studying the Mechanisms of Action of Artificial Electromagnetic Fields on Cell

Shckorbatov¹

2014

J Elec Electron Syst

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Transient effect of weak electromagnetic fields on calcium ion concentration in Arabidopsis thaliana

Cited by 37 publications

References 36 publications

Growth promotion and a decrease of oxidative stress in maize seedlings by a combination of geomagnetic and weak electromagnetic fields

Growth promotion and a decrease of oxidative stress in maize seedlings by a combination of geomagnetic and weak electromagnetic fields

Cell nucleus and membrane recovery after exposure to microwaves

The Main Approaches of Studying the Mechanisms of Action of Artificial Electromagnetic Fields on Cell

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