Transient exposure to sodium butyrate after germinal vesicle breakdown improves meiosis but not developmental competence in pig oocytes

Liu, Limei; Song, Guangqi; Gao, Fei; Guan, Jiyu; Tang, Bo; Li, Ziyi

doi:10.1042/cbi20110220

Cited by 14 publications

(6 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results suggest that NaBu treatment will have a harmful effect on the meiosis of oocytes. This is consistent with the previous study by Liu et al, in which they reported that NaBu can delay the meiosis of oocytes in both the GV and GVBD stages in an exposure-dependent manner in porcine oocytes [38]. The harmful effects of NaBu on meiosis is similar to those of TSA, another HDACi.…”

Section: Discussionsupporting

confidence: 92%

Sodium butyrate interrupts the maturation of oocytes and enhances the development of preimplantation embryos

Wang

et al. 2019

PLoS ONE

View full text Add to dashboard Cite

Histone acetylation is one of the most important posttranslational modifications that contribute to transcriptional initiation and chromatin remodeling. In the present study, we aimed to investigate the effect of sodium butyrate (NaBu), a natural histone deacetylase inhibitor (HDACi), on the maturation of oocytes, preimplantation embryonic development, and expression of important developmental genes. The results indicated that NaBu decreased the rates of GVBD and the first polar body extrusion (PBE) in vitro in a dose-dependent manner. Meanwhile, NaBu treatment led to an abnormality in the spindle apparatus in oocytes in MI. However, the ratio of phosphor-extracellular signal-regulated kinases (p-ERK)/ERK significantly decreased in oocytes treated with 2.0 mM NaBu for 8 h. Furthermore, NaBu treatment at 2.0 mM improved the quality of embryos and the mRNA expression levels of important developmental genes such as HDAC1 , Sox2 , and Pou5f1 . These data suggest that although a high concentration NaBu will impede the meiosis of oocytes, 2.0 mM NaBu will promote the development of embryos in vitro . Further investigation is needed to clarify the direct/indirect effects of NaBu on the regulation of important developmental genes and their subsequent impacts on full-term development in mammals.

show abstract

Section: Discussionsupporting

confidence: 92%

Sodium butyrate interrupts the maturation of oocytes and enhances the development of preimplantation embryos

Wang

et al. 2019

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…The follicular fluid containing cumulus-oocyte complexes (COCs) from 3 to 6 mm porcine ovarian follicles was aspirated using an 18-gauge needle. COCs, whose cumulus cells form at least three layers, were selected, washed three times in manipulation fluid (TCM-199 supplemented with 0.1% polyvinyl alcohol), and then cultured in in vitro maturation (IVM) media (Liu et al, 2012). Approximately 30 COCs were each cultured in a 100 μl drop of maturation medium (TCM-199 supplemented with 10 μg/ml epidermal growth factor, 0.5 μg/ml porcine luteinizing hormone, 0.5 μg/ml porcine follicle-stimulating hormone, 26 mmol/L sodium bicarbonate, 3.05 mmol/L glucose, 0.91 mmol/L sodium pyruvate, 0.57 mmol/L cysteine, 0.1% PVA, 10% fetal calf serum, 75 mg/ml penicillin G and 50 mg/ml streptomycin) for 22-24 hr and then cultured with hormone-free maturation medium (TCM-199 supplemented with 26 mmol/L sodium bicarbonate, 3.05 mmol/L glucose, 0.91 mmol/L sodium pyruvate, 0.57 mmol/L cysteine, 10 μg/ml epidermal growth factor, 0.1% (w/v) PVA, 0.03% (w/v) BSA, and 100 μl/ml penicillin/streptomycin) for 20 hr at 38.5°C and 5% CO 2 .…”

Section: Oocytecollectionandinvitromaturationmentioning

confidence: 99%

MicroRNA‐29b regulatesDNAmethylation by targeting Dnmt3a/3b and Tet1/2/3 in porcine early embryo development

et al. 2018

Self Cite

View full text Add to dashboard Cite

MicroRNA-29b (miR-29b) is a member of the miR-29 family, which targets DNA methyltransferases (DNMTs) and ten eleven translocation enzymes (TETs), thereby regulating DNA methylation. However, the role of miR-29b in porcine early embryo development has not been reported. In this study, we examined the effects of miR-29b in porcine in vitro fertilization (IVF) embryos to investigate the mechanism by which miR-29b regulated DNA methylation. The interference of miR-29b by its special miRNA inhibitor significantly up-regulated Dnmt3a/b and Tet1 but downregulated Tet2/3; meanwhile it increased DNA methylation levels of the global genome and Nanog promoter region but decreased global DNA demethylation levels. The inhibition of miR-29b also resulted in a decrease in the development rate and quality of blastocysts. In addition, the pluripotency genes Nanog and Sox2 were significantly downregulated, and the apoptosis genes Bax and Casp3 were upregulated, but anti-apoptosis gene Bcl-2 was downregulated in blastocysts. Our study indicated that miR-29b could regulate DNA methylation mediated by miR29b- Dnmt3a/b - Tet1/2/3 signaling during porcine early embryo development.

show abstract

“…The follicular fluid containing cumulus-oocyte complexes (COCs) from 3-6 mm ovarian follicles was aspirated using an 18-gauge needle. COCs with at least three layers of cumulus cells were selected, washed three times in manipulation fluid (TCM-199 supplemented with 0.1% polyvinyl alcohol), and then cultured in in vitro maturation (IVM) media [18]. Approximately 30 COCs were each cultured in a 100 μL drop of maturation medium (TCM-199 supplemented with 10 µg/mL epidermal growth factor, 0.5 μg/mL porcine luteinizing hormone, 0.5 μg/mL porcine follicle-stimulating hormone, 26 mM sodium bicarbonate, 3.05 mM glucose, 0.91 mM sodium pyruvate, 0.57 mM cysteine, 0.1% PVA, 10% foetal calf serum, 75 mg/mL penicillin G and 50 mg/mL streptomycin) for 22-24 h and then cultured with hormone-free maturation medium (TCM-199 supplemented with 26 mM sodium bicarbonate, 3.05 mM glucose, 0.91 mM sodium pyruvate, 0.57 mM cysteine, 10 µg/ml epidermal growth factor, 0.1% (w/v) PVA, 0.03% (w/v) BSA, and 100 µl/mL penicillin/streptomycin) for 20 h at 38.5 °C and 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Down-Regulation of H3K4me3 by MM-102 Facilitates Epigenetic Reprogramming of Porcine Somatic Cell Nuclear Transfer Embryos

Zhang¹,

Zhai²,

Ma³

et al. 2018

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

Background/Aims: Aberrantly high levels of H3K4me3, caused by incomplete epigenetic reprogramming, likely cause a low efficiency of somatic cell nuclear transfer (SCNT). Smal molecule inhibitors aimed at epigenetic modification can be used to improve porcine SCNT embryo development. In this study, we examined the effects of MM-102, an H3K4 histone methyltransferase inhibitor, on porcine SCNT preimplantation embryos to investigate the mechanism by which H3K4 methylation regulated global epigenetic reprograming during SCNT. Methods: MM-102 was added to the SCNT embryos culture system and the global levels of various epigenetic modifications were measured by immunofluorescence (IF) staining and were quantified by Image J software. Relative genes expression levels were detected by quantitative real-time PCR. Results: MM-102 (75 μM) treatment reduced global H3K4, H3K9 methylation and 5mC levels especially at the zygotic gene activation (ZGA) and blastocyst stages. MM-102 treatment mainly down-regulated a series of DNA and histone methyltransferases, and up-regulated a number of hitone acetyltransferases and transcriptional activators. Furthermore, MM-102 treatment positively regulated the mRNA expression of genes related to pluripotency (OCT4, NANOG, CDX2) and apoptosis (BCL2). Conclusion: Down-regulation of H3K4me3 with MM-102 rescued aberrant gene expression patterns of a series of epigenetic chromatin modification enzymes, pluripotent and apoptotic genes at the ZGA and blastocyst stages, thereby greatly improving porcine SCNT efficiency and blastocyst quality, making them more similar to in vivo embryos (IVV).

show abstract

Transient exposure to sodium butyrate after germinal vesicle breakdown improves meiosis but not developmental competence in pig oocytes

Cited by 14 publications

References 35 publications

Sodium butyrate interrupts the maturation of oocytes and enhances the development of preimplantation embryos

Sodium butyrate interrupts the maturation of oocytes and enhances the development of preimplantation embryos

MicroRNA‐29b regulatesDNAmethylation by targeting Dnmt3a/3b and Tet1/2/3 in porcine early embryo development

Down-Regulation of H3K4me3 by MM-102 Facilitates Epigenetic Reprogramming of Porcine Somatic Cell Nuclear Transfer Embryos

Contact Info

Product

Resources

About