We report the experimental demonstration of an Ytterbium doped fiber laser (YDFL) composed by two stages in cascade. Two sorts of Ytterbium doped fiber (YDF) with different concentration have been used. A proper characterization of the YDFs has been performed in order to find the optimum YDF length. To this end, the output power and the slope-efficiency have been calculated and the signal amplification and the amplified spontaneous emission (ASE) spectra have been analyzed, which are strictly related to the absorption and emission cross-section of the YDF. The first stage has been studied for both continuous wave (CW) and pulsed regime. The results show that by using an YDF with a high slope-efficiency, up to 66%, it is possible to reach an emitted peak power of 30 W in a pulsed regime.