Transient induction of tryptophan decarboxylase (TDC) and strictosidine synthase (SS) genes in cell suspension cultures of Catharanthus roseus

Roewer, I. A.; Cloutier, Nicole; Nessler, Craig L.; Luca, Vincenzo De

doi:10.1007/bf00235259

Cited by 63 publications

(19 citation statements)

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“…In suspension cells, the perception of yeast extract (YE) leads to the activation of terpenoid indole alkaloid biosynthesis (5). Two genes involved in terpenoid indole alkaloid biosynthesis, encoding strictosidine synthase (STR) and tryptophan decarboxylase (TDC), are coordinately regulated and their mRNAs accumulate transiently after YE treatment (6,7). Induction of these genes by YE is mediated by protein phosphorylation, the influx of calcium, and the biosynthesis of JA via the octadecanoid pathway (3,8).…”

mentioning

confidence: 99%

Zinc Finger Proteins Act as Transcriptional Repressors of Alkaloid Biosynthesis Genes in Catharanthus roseus

Pauw

Hilliou

Martin

et al. 2004

Journal of Biological Chemistry

162

114

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In Catharanthus roseus cell suspensions, the expression of several terpenoid indole alkaloid biosynthetic genes, including two genes encoding strictosidine synthase (STR) and tryptophan decarboxylase (TDC), is coordinately induced by fungal elicitors such as yeast extract. To identify molecular mechanisms regulating the expression of these genes, a yeast one-hybrid screening was performed with an elicitor-responsive part of the TDC promoter. This screening identified three members of the Cys 2 /His 2 -type (transcription factor IIIA-type) zinc finger protein family from C. roseus, ZCT1, ZCT2, and ZCT3. These proteins bind in a sequence-specific manner to the TDC and STR promoters in vitro and repress the activity of these promoters in trans-activation assays. In addition, the ZCT proteins can repress the activating activity of APETALA2/ethylene responsefactor domain transcription factors, the ORCAs, on the STR promoter. The expression of the ZCT genes is rapidly induced by yeast extract and methyljasmonate. These results suggest that the ZCT proteins act as repressors in the regulation of elicitor-induced secondary metabolism in C. roseus.

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mentioning

confidence: 99%

Zinc Finger Proteins Act as Transcriptional Repressors of Alkaloid Biosynthesis Genes in Catharanthus roseus

Pauw

Hilliou

Martin

et al. 2004

Journal of Biological Chemistry

162

114

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“…TDC activity is also highly regulated in Catharanthus and Cinchona cell culture, where under certain conditions, transient activation of the enzyme can occur (8,13,16,21). The appearance of this enzyme in Catharanthus cultures has been shown to be preceded by transient appearance of TDC mRNA after induction by fungal elicitors (20).…”

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confidence: 99%

Auxins Induce Tryptophan Decarboxylase Activity in Radicles of Catharanthus Seedlings

Aerts

Alarco²,

Luca³

1992

Plant Physiol.

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Germinating seedlings of Catharanthus roseus produce monoterpenoid indole alkaloids as a result of a transient increase of tryptophan decarboxylase (TDC) activity. The influence of auxins on this transient rise of TDC activity was studied. External application of indolebutyric acid or 2,4-dichlorophenoxyacetic acid at a concentration of 20 to 40 Mm enhanced and prolonged the rise in TDC activity in developing seedlings. Auxin treatment also influenced the morphology of the seedlings; it induced a shortening and thickening of the hypocotyl and the radicle and promoted the initiation of lateral roots in the radicle. During development, the radicles of auxin-treated seedlings displayed a gradual increase in TDC activity that was absent in the radicles of untreated controls. Examination of immunoblots revealed anti-TDC reactive proteins in extracts from radicles of auxin-treated seedlings, but none in extracts from radicles of control seedlings. In contrast, TDC activity and immunoreactive protein levels in the aerial parts of controls and auxin-treated seedlings were comparable. Our results indicate that externally applied auxins induce both abnormal development and TDC activity in the radicles of Catharanthus seedlings. Although auxins slightly delayed the light-mediated induction of the cotyledon-specific last step in vindoline biosynthesis (i.e. acetylcoenzyme A: deacetylvindolin-O-acetyltransferase activity), seedlings still synthesized vindoline, one of the major alkaloid end products.

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“…High dosage of elicitor has been reported to induce hypersensitive response leading to cell death. Therefore, an optimum level was required for induction of secondary metabolite biosynthesis in plant cell cultures (Mukundan and Hjortsø 1990;Roewer et al 1992). These results also indicate the importance of elicitor specificity in its selection for optimum induction of desired phytochemicals in plant cell cultures.…”

Section: Effect Of Co-cultivationmentioning

confidence: 82%

Effect of Piriformospora indica on Enhanced Biosynthesis of Anticancer Drug, Podophyllotoxin, in Plant Cell Cultures of Linum album

2013

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Transient induction of tryptophan decarboxylase (TDC) and strictosidine synthase (SS) genes in cell suspension cultures of Catharanthus roseus

Cited by 63 publications

References 10 publications

Zinc Finger Proteins Act as Transcriptional Repressors of Alkaloid Biosynthesis Genes in Catharanthus roseus

Zinc Finger Proteins Act as Transcriptional Repressors of Alkaloid Biosynthesis Genes in Catharanthus roseus

Auxins Induce Tryptophan Decarboxylase Activity in Radicles of Catharanthus Seedlings

Effect of Piriformospora indica on Enhanced Biosynthesis of Anticancer Drug, Podophyllotoxin, in Plant Cell Cultures of Linum album

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