Transient loading of CD34&lt;sup&gt;+&lt;/sup&gt;&amp;nbsp;hematopoietic progenitor cells with polystyrene nanoparticles

Deville, Sarah; Hadiwikarta, Wahyu Wijaya; Smisdom, Nick; Wathiong, Bart; Ameloot, Marcel; Nelissen, Inge; Hooyberghs, Jef

doi:10.2147/ijn.s119407

Cited by 5 publications

(15 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As described previously [26], CD34+ HPCs were demonstrated to associate in a transient manner with 40 nm PS NPs in contrast to dendritic cells. Cell-associated fluorescence intensity (FI), originating upon exposure to PS NPs, reached a maximum after ~ 1 h and declined afterwards, suggesting prompt accumulation of NPs by the cells followed by NP release.…”

Section: Resultssupporting

confidence: 59%

“…To study whether this observation was dependent on NP size, we exposed HPCs to 50 µg/ml of 40, 100 and 200 nm PS NPs. The latter concentration was selected based on our previous work [26], in which it was demonstrated to generate a potent loading capacity of HPCs with 40 nm PS NPs in the linear part of a dose–response curve after 24 h of exposure. In parallel, we performed a similar experiment with THP-1 monocytes to compare the cell-specific time course of the association between PS NPs of different size and cells.…”

Section: Resultsmentioning

confidence: 99%

“…To our knowledge, there are no research data available demonstrating the involvement of membrane-attached glycan molecules in the interaction of HPCs with NPs. In our previous study [26], we observed a transient loading behavior upon exposure of HPCs to carboxylated 40 nm polystyrene (PS) NPs. The loading was shown to rely on energy-dependent cellular processes.…”

Section: Introductionmentioning

confidence: 99%

“…The aim of the current study is to investigate the possible role of the membrane-associated glycans and NP size on the transient PS NP loading of HPCs. To this end, HPCs were exposed to 40, 100 and 200 nm PS NPs which are chemically identical to the 40 nm PS NPs as used in our previous work [26]. We furthermore studied the loading behavior after cells were treated with the proteoglycan-degrading enzyme neuraminidase.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Role of nanoparticle size and sialic acids in the distinct time-evolution profiles of nanoparticle uptake in hematopoietic progenitor cells and monocytes

et al. 2019

Self Cite

View full text Add to dashboard Cite

Background Human hematopoietic progenitor cells (HPCs) are important for cell therapy in cancer and tissue regeneration. In vitro studies have shown a transient association of 40 nm polystyrene nanoparticles (PS NPs) with these cells, which is of interest for intelligent design and application of NPs in HPC-based regenerative protocols. In this study, we aimed to investigate the involvement of nanoparticles’ size and membrane-attached glycan molecules in the interaction of HPCs with PS NPs, and compared it with monocytes. Human cord blood-derived HPCs and THP-1 cells were exposed to fluorescently labelled, carboxylated PS NPs of 40, 100 and 200 nm. Time-dependent nanoparticle membrane association and/or uptake was observed by measuring fluorescence intensity of exposed cells at short time intervals using flow cytometry. By pretreating the cells with neuraminidase, we studied the possible effect of membrane-associated sialic acids in the interaction with NPs. Confocal microscopy was used to visualize the cell-specific character of the NP association. Results Confocal images revealed that the majority of PS NPs was initially observed to be retained at the outer membrane of HPCs, while the same NPs showed immediate internalization by THP-1 monocytic cells. After prolonged exposure up to 4 h, PS NPs were also observed to enter the HPCs’ intracellular compartment. Cell-specific time courses of NP association with HPCs and THP-1 cells remained persistent after cells were enzymatically treated with neuraminidase, but significantly increased levels of NP association could be observed, suggesting a role for membrane-associated sialic acids in this process. Conclusions We conclude that the terminal membrane-associated sialic acids contribute to the NP retention at the outer cell membrane of HPCs. This retention behavior is a unique characteristic of the HPCs and is independent of NP size. Electronic supplementary material The online version of this article (10.1186/s12951-019-0495-x) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultssupporting

confidence: 59%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Role of nanoparticle size and sialic acids in the distinct time-evolution profiles of nanoparticle uptake in hematopoietic progenitor cells and monocytes

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Nowadays, there are numerous delivery systems such as: natural biological vehicles (such as bacteria and viruses [1,2], various cell types including red blood cells (RBCs) [3][4][5], immune cells [6,7], stem cells [8][9][10][11] and manufactured carriers (liposomes [12], micelle [13], polymeric capsules [14][15][16][17], polymeric complexes [18][19][20][21]). Polymeric nanocapsules (NCs) are a promising system of natural carriers for targeted drug delivery because of their low cytotoxicity, shell flexibility, and high in vivo stability [22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Impact of Nanocapsules on Red Blood Cells Interplay Jointly Assessed by Optical Tweezers and Microscopy

et al. 2019

View full text Add to dashboard Cite

In the framework of novel medical paradigm the red blood cells (RBCs) have a great potential to be used as drug delivery carriers. This approach requires an ultimate understanding of the peculiarities of mutual interaction of RBC influenced by nano-materials composed the drugs. Optical tweezers (OT) is widely used to explore mechanisms of cells’ interaction with the ability to trap non-invasively, manipulate and displace living cells with a notably high accuracy. In the current study, the mutual interaction of RBC with polymeric nano-capsules (NCs) is investigated utilizing a two-channel OT system. The obtained results suggest that, in the presence of NCs, the RBC aggregation in plasma satisfies the ‘cross-bridges’ model. Complementarily, the allocation of NCs on the RBC membrane was observed by scanning electron microscopy (SEM), while for assessment of NCs-induced morphological changes the tests with the human mesenchymal stem cells (hMSC) was performed. The combined application of OT and advanced microscopy approaches brings new insights into the conception of direct observation of cells interaction influenced by NCs for the estimation of possible cytotoxic effects.

show abstract

Metabolomic characteristics in human CD34+ hematopoietic stem/progenitor cells exposed to polystyrene nanoplastics

Guo

Cheng

Lin

et al. 2023

Food and Chemical Toxicology

View full text Add to dashboard Cite

Transient loading of CD34<sup>+</sup> hematopoietic progenitor cells with polystyrene nanoparticles

Cited by 5 publications

References 42 publications

Role of nanoparticle size and sialic acids in the distinct time-evolution profiles of nanoparticle uptake in hematopoietic progenitor cells and monocytes

Role of nanoparticle size and sialic acids in the distinct time-evolution profiles of nanoparticle uptake in hematopoietic progenitor cells and monocytes

Impact of Nanocapsules on Red Blood Cells Interplay Jointly Assessed by Optical Tweezers and Microscopy

Metabolomic characteristics in human CD34+ hematopoietic stem/progenitor cells exposed to polystyrene nanoplastics

Contact Info

Product

Resources

About