Transient Migration of Large Numbers of CD14++ CD16+ Monocytes to the Draining Lymph Node after Onset of Inflammation

Lund, Hege; Boysen, Preben; Åkesson, Caroline Piercey; Lewandowska-Sabat, Anna; Storset, Anne K.

doi:10.3389/fimmu.2016.00322

Cited by 29 publications

(28 citation statements)

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“…Plasma from diseased animals induced an elevated expression of genes encoding for the inflammatory mediators CXCL8, CXCL1 , and IL1B in intM ( 90 ). The clinical relevance of bovine intM as a pro-inflammatory monocyte with an important role during the acute phase of inflammation has been confirmed in a recent work on calves with adjuvant-induced skin inflammation ( 91 ). The study has demonstrated a potent recruitment of bovine intM to the draining lymph node after the onset of a local skin inflammation with upregulated genes for pro-inflammatory cytokines.…”

Section: Clinical Relevance Of Bovine Monocyte Subsetsmentioning

confidence: 84%

Heterogeneity of Bovine Peripheral Blood Monocytes

Hussen

Schuberth

2017

Front. Immunol.

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Peripheral blood monocytes of several species can be divided into different subpopulations with distinct phenotypic and functional properties. Herein, we aim at reviewing published work regarding the heterogeneity of the recently characterized bovine monocyte subsets. As the heterogeneity of human blood monocytes was widely studied and reviewed, this work focuses on comparing bovine monocyte subsets with their human counterparts regarding their phenotype, adhesion and migration properties, inflammatory and antimicrobial functions, and their ability to interact with neutrophilic granulocytes. In addition, the differentiation of monocyte subsets into functionally polarized macrophages is discussed. Regarding phenotype and distribution in blood, bovine monocyte subsets share similarities with their human counterparts. However, many functional differences exist between monocyte subsets from the two species. In contrast to their pro-inflammatory functions in human, bovine non-classical monocytes show the lowest phagocytosis and reactive oxygen species generation capacity, an absent ability to produce the pro-inflammatory cytokine IL-1β after inflammasome activation, and do not have a role in the early recruitment of neutrophils into inflamed tissues. Classical and intermediate monocytes of both species also differ in their response toward major monocyte-attracting chemokines (CCL2 and CCL5) and neutrophil degranulation products (DGP) in vitro. Such differences between homologous monocyte subsets also extend to the development of monocyte-derived macrophages under the influence of chemokines like CCL5 and neutrophil DGP. Whereas the latter induce the differentiation of M1-polarized macrophages in human, bovine monocyte-derived macrophages develop a mixed M1/M2 macrophage phenotype. Although only a few bovine clinical trials analyzed the correlation between changes in monocyte composition and disease, they suggest that functional differences between human and bovine monocyte subsets are also reflected in their different clinical relevance for distinct diseases. In opposite to the human system, where higher blood cell number of non-classical monocytes was widely correlated with several human infectious and non-infectious diseases, higher counts of bovine intermediate monocytes are suggested as a potential biomarker for inflammatory responses postpartum.

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Section: Clinical Relevance Of Bovine Monocyte Subsetsmentioning

confidence: 84%

Heterogeneity of Bovine Peripheral Blood Monocytes

Hussen

Schuberth

2017

Front. Immunol.

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“…The major CD14 high CD16 neg classical monocyte population is rapidly recruited to sites of inflammation or tissue damage, while the less-frequent CD14 low CD16 high non-classical monocytes exhibit vascular surveillance functions during steady state. The CD14 high CD16 dim “intermediate” monocytes [ 14 ] are the least abundant monocyte population, although their abundance can vary in pathological conditions [ 15 ]. Recent studies of gene expression profiles of the monocyte subsets [ 16 – 18 ] and of their kinetics in blood [ 19 ] have shown that the monocyte population in blood is a developmental continuum.…”

Section: Isolation and Characterization Of Major Mdsc Types From Perimentioning

confidence: 99%

Deciphering myeloid-derived suppressor cells: isolation and markers in humans, mice and non-human primates

Cassetta

Bækkevold

Brandau

et al. 2019

Cancer Immunol Immunother

180

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In cancer, infection and inflammation, the immune system’s function can be dysregulated. Instead of fighting disease, immune cells may increase pathology and suppress host-protective immune responses. Myeloid cells show high plasticity and adapt to changing conditions and pathological challenges. Despite their relevance in disease pathophysiology, the identity, heterogeneity and biology of myeloid cells is still poorly understood. We will focus on phenotypical and functional markers of one of the key myeloid regulatory subtypes, the myeloid derived suppressor cells (MDSC), in humans, mice and non-human primates. Technical issues regarding the isolation of the cells from tissues and blood, timing and sample handling of MDSC will be detailed. Localization of MDSC in a tissue context is of crucial importance and immunohistochemistry approaches for this purpose are discussed. A minimal antibody panel for MDSC research is provided as part of the Mye-EUNITER COST action. Strategies for the identification of additional markers applying state of the art technologies such as mass cytometry will be highlighted. Such marker sets can be used to study MDSC phenotypes across tissues, diseases as well as species and will be crucial to accelerate MDSC research in health and disease.

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“…They are known as a heterogeneous group of cells consisting of multiple subsets generated through distinct developmental pathways with diverse functional specializations 3 . Human monocytes consist of phenotypically and functionally distinct subpopulations, a varying amount of which is observed in the blood of patients in numerous pathological states, such as infections, autoimmune diseases, respiratory and cardiovascular diseases and inflammatory disorders 4,5 . During homeostasis and under pathological conditions, each subpopulation may play a different role.…”

Section: Introductionmentioning

confidence: 99%

Pattern of human monocyte subpopulations in health and disease

2020

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Monocytes are important cells of the innate system. They are a heterogeneous type of cells consisting of phenotypically and functionally distinct subpopulations, which play a specific role in the control, development and escalation of the immunological processes. Based on the expression of superficial CD14 and CD16 in flow cytometry, they can be divided into three subsets: classical, intermediate and non‐classical. Variation in the levels of human monocyte subsets in the blood can be observed in patients in numerous pathological states, such as infections, cardiovascular and inflammatory diseases, cancer and autoimmune diseases. The aim of this review is to summarize current knowledge of human monocyte subsets and their significance in homeostasis and in pathological conditions.

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Transient Migration of Large Numbers of CD14++ CD16+ Monocytes to the Draining Lymph Node after Onset of Inflammation

Cited by 29 publications

References 50 publications

Heterogeneity of Bovine Peripheral Blood Monocytes

Heterogeneity of Bovine Peripheral Blood Monocytes

Deciphering myeloid-derived suppressor cells: isolation and markers in humans, mice and non-human primates

Pattern of human monocyte subpopulations in health and disease

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