Transient receptor potential channels in the inner ear: presence of transient receptor potential channel subfamily 1 and 4 in the guinea pig inner ear

Takumida, Masaya; Kubo, Nobuo; Ohtani, Makiko; Suzuka, Yuko; Anniko, Matti

doi:10.1080/00016480510038572

Cited by 55 publications

(48 citation statements)

References 19 publications

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“…Recent studies show that another TRP channel, the mechanosensitive TRPV4, is present in the stria vascularis. [17][18][19] Given this background, we set out to determine if TRPV4 is aminoglycoside-permissive, and its cellular distribution in the stria vascularis.…”

mentioning

confidence: 99%

Intra-cochlear trafficking of aminoglycosides

Steyger¹,

Karasawa²

2008

Communicative & Integrative Biology

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mentioning

confidence: 99%

Intra-cochlear trafficking of aminoglycosides

Steyger¹,

Karasawa²

2008

Communicative & Integrative Biology

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“…Members of the TRPV channel family have been shown to play a role in sensory transduction as well as in other non-sensory pathways. TRPV4 in particular has been shown to be expressed in inner and outer hair cells, vestibular hair cells, stria vascularis, and vestibular dark cells (Liedtke et al 2000;Takumida et al 2005). Our RNA expression screen revealed spatial and/or temporal gradients in expression for most of the TRPV channels (Fig.…”

Section: Trpv Familymentioning

confidence: 82%

“…Analysis of two TRP genes, TRPV4 and TRPML3, expressed in the ear revealed important nonsensory roles for TRPs in hearing and balance. TRPV4 is expressed in hair cells, stria vascularis, and vestibular dark cells (Liedtke et al 2000;Takumida et al 2005), and disruption of TRPV4 causes delayed-onset hearing loss and increases susceptibility to acoustic injury (Tabuchi et al 2005). TRPML3 is expressed in sensory hair cells and in the marginal cells of the stria vascularis (Di Palma et al 2002;Nagata et al 2008;Van Aken et al 2008).…”

Section: Introductionmentioning

confidence: 99%

A Quantitative Analysis of the Spatiotemporal Pattern of Transient Receptor Potential Gene Expression in the Developing Mouse Cochlea

Asai

Holt

Géléoc

2009

JARO

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TRP genes encode a diverse family of ion channels which have been implicated in many sensory functions. Because several TRP channels have similar properties to the elusive hair cell transduction channel, recent attention has focused on TRP gene expression in the inner ear. At least four TRP genes are known to be expressed in hair cells: TRPC3, TRPV4, TRPA1, and TRPML3. However, there is little evidence supporting any of these as a component of the transduction complex. Other less well-characterized TRP channels are expressed in the inner ear, in particular, within the organ of Corti. Because of their potential role in sensory function, we investigated the developmental expression of RNA that encodes all 33 TRP subunits as well as several splice variants. We designed a quantitative PCR screen using cochlear samples acquired before, during, and after the time when mechanotransduction is acquired in sensory hair cells (embryonic day 17 to postnatal day 8). Cochleas, which included the organ of Corti, stria vascularis, and Reissner's membrane, were subdivided into four equal quadrants which allowed for regional comparison during development. Expression of RNA transcripts that encoded 33 TRP subunits plus several splice forms and beta-actin were quantified in 28 samples for a total of 1,092 individual measurements, each done in triplicate. We detected RNA that encoded all TRP channels except two: TRPC7 and TRPM8. The largest changes in RNA expression were for TRPA1 (9100-fold), which suggested that these subunits may contribute to normal cochlear function. Furthermore, the screen revealed TRPP3 and PKD1L3 RNA expression patterns that were correlated with the acquisition of sensory transduction in outer hair cells (Lelli et al., J Neurophysiol. 101:2961-2973, 2009). Numerous spatiotemporal expression gradients were identified many of which may contribute to the normal functional development of the mouse cochlea.

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“…In the vestibular system, histological experiments have shown that TRPV4 is expressed in the vestibular ganglion (VG), endolymphatic sac, sensory cells, and dark cells in the vestibular end organs [Ishibashi et al, 2009;Kitahara et al, 2005;Kumagami et al, 2009;Taguchi et al, 2007;Takumida et al, 2005], and a significant downregulation of TRPV4 mRNA was observed in the mouse VG after kanamycin treatment [Kitahara et al, 2005]. However, it is unknown whether TRPV4 channels in VG neurons can be stimulated by 4α-PDD and hypotonicity, such as those in the mouse outer hair cells.…”

Section: Introductionmentioning

confidence: 99%

“…In the guinea pig and mouse cochlea, TRPV4 is expressed in the inner and outer hair cells and spiral ganglion neurons [Liedtke et al, 2003;Ishibashi et al, 2009;Kitahara et al, 2005;Shen et al, 2006;Takumida et al, 2005], and Ca 2+ imaging experiments have revealed functional TRPV4 expression in outer hair cells [Shen et al, 2006]. Experiments using TRPV4 knockout mice have shown that its disruption induces delayed-onset hearing loss and renders the cochlea vulnerable to acoustic injury [Tabuchi et al, 2005], indicating that the channel is essential for the prevention of sensorineural hearing loss.…”

Section: Introductionmentioning

confidence: 99%

Functional Expression of an Osmosensitive Cation Channel, Transient Receptor Potential Vanilloid 4, in Rat Vestibular Ganglia

Kamakura

Kondo²,

Koyama³

et al. 2016

Audiol Neurotol

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Transient receptor potential vanilloid (TRPV) 4 is a nonselective cation channel expressed in sensory neurons such as those in the dorsal root and trigeminal ganglia, kidney, and inner ear. TRPV4 is activated by mechanical stress, heat, low osmotic pressure, low pH, and phorbol derivatives such as 4α-phorbol 12,13-didecanoate (4α-PDD). We investigated the expression of TRPV4 in rat vestibular ganglion (VG) neurons. The TRPV4 gene was successfully amplified from VG neuron mRNA using reverse-transcription polymerase chain reaction. Furthermore, immunoblotting showed positive expression of TRPV4 protein in VG neurons. Immunohistochemistry indicated that TRPV4 was localized predominantly on the plasma membrane of VG neurons. Calcium (Ca²⁺) imaging of VG neurons showed that 4α-PDD and/or hypotonic stimuli caused an increase in intracellular Ca²⁺ concentration ([Ca²⁺]_i) that was almost completely inhibited by ruthenium red, a selective antagonist of TRPV channels. Interestingly, a [Ca²⁺]_i increase was evoked by both hypotonic stimuli and 4α-PDD in approximately 38% of VG neurons. These data indicate that TRPV4 is functionally expressed in VG neurons as an ion channel and that TRPV4 likely participates in VG neurons for vestibular neurotransmission as an osmoreceptor and/or mechanoreceptor.

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Transient receptor potential channels in the inner ear: presence of transient receptor potential channel subfamily 1 and 4 in the guinea pig inner ear

Cited by 55 publications

References 19 publications

Intra-cochlear trafficking of aminoglycosides

Intra-cochlear trafficking of aminoglycosides

A Quantitative Analysis of the Spatiotemporal Pattern of Transient Receptor Potential Gene Expression in the Developing Mouse Cochlea

Functional Expression of an Osmosensitive Cation Channel, Transient Receptor Potential Vanilloid 4, in Rat Vestibular Ganglia

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