The operating temperature plays a key role in the service reliability of ball bearings used in the traction motor of high-speed trains. Studies on the heat generation and temperature distributions of the bearing under the vehicle vibration environment are critical for designing the high-speed train traction motor bearing and to monitor its operation. In this paper, an SKF friction torque model that considers the inlet shear heating reduction and kinematic replenishment/starvation reduction was used to calculate the power loss of the bearing operating in a high-speed train. A thermal analysis model of the bearing, which takes into account the vehicle vibration due to track irregularities, was developed to study the thermal characteristics of the bearing operating in a high-speed train. Experiments were conducted on a service high-speed train (i.e. CRH380B) to validate the proposed bearing thermal analysis model. The thermal characteristics of the bearing and the influence of key factors on the operating temperature of the bearing under vehicle vibration due to track irregularities were studied with the proposed bearing thermal analysis model. The results show that the thermal failure of the bearing subassemblies most likely occurs at the balls region owing to the highest temperature in that region, compared to the inner and outer raceway of the bearing. The method of applying grease with appropriate kinematic viscosity should be adopted to reduce the power loss of bearing while meeting the lubrication requirement. The vehicle vibration due to track irregularities significantly influences the thermal characteristics of high-speed train motor bearings, which cannot be ignored in the thermal analysis of bearings operating in a high-speed train.