Transinfection of buffalo flies (Haematobia irritans exigua) with Wolbachia and effect on host biology

Madhav, Mukund; Brown, Geoffrey W.; Morgan, Jess A. T.; Asgari, Sassan; McGraw, Elizabeth A.; James, Peter

doi:10.1186/s13071-020-04161-8

Cited by 11 publications

(4 citation statements)

References 77 publications

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“…Currently, three strains of Wolbachia ( w AlbB, w Mel and w MelPop) have been successfully injected into adults and pupae of Buffalo flies ( Haematobia irritans exigua ) and the closely-related horn flies ( Haematobia irritans irritans ). The bacterially-induced reduced fitness, such as decreased and delayed adult emergence, reduced longevity, and reduced fecundity in Wolbachia -infected flies, suggest the potential of the w Mel or w MelPop strains for use in Wolbachia -based biocontrol programs for suppressing Buffalo fly populations [ 109 ]. It is worth mentioning that high fitness costs for released males are detrimental to insect control with Wolbachia .…”

Section: Discussionmentioning

confidence: 99%

A survey of Wolbachia infection in brachyceran flies from Iran

Khosravi,

Akbarzadeh,

Karimian

et al. 2024

PLoS ONE

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Wolbachia is a maternally inherited intracellular bacterium that is considered to be the most plentiful endosymbiont found in arthropods. It reproductively manipulates its host to increase the chances of being transmitted to the insect progeny; and it is currently used as a means of suppressing disease vector populations or controlling vector-borne diseases. Studies of the dissemination and prevalence of Wolbachia among its arthropod hosts are important for its possible use as a biological control agent. The molecular identification of Wolbachia relies on different primers sets due to Wolbachia strain variation. Here, we screened for the presence of Wolbachia in a broad range of Brachycera fly species (Diptera), collected from different regions of Iran, using nine genetic markers (wsp, ftsZ, fbpA, gatB, CoxA, gltA, GroEL dnaA, and 16s rRNA), for detecting, assessing the sensitivity of primers for detection, and phylogeny of this bacterium. The overall incidence of Wolbachia among 22 species from six families was 27.3%. The most commonly positive fly species were Pollenia sp. and Hydrotaea armipes. However, the bacterium was not found in the most medically important flies or in potential human disease vectors, including Musca domestica, Sarcophaga spp., Calliphora vicinia, Lucilia sericata, and Chrysomya albiceps. The primer sets of 16s rRNA with 53.0% and gatB with 52.0% were the most sensitive primers for detecting Wolbachia. Blast search, phylogenetic, and MLST analysis of the different locus sequences of Wolbachia show that all the six distantly related fly species likely belonging to supergroup A. Our study showed some primer sets generated false negatives in many of the samples, emphasizing the importance of using different loci in detecting Wolbachia. The study provides the groundwork for future studies of a Wolbachia-based program for control of flies.

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Section: Discussionmentioning

confidence: 99%

A survey of Wolbachia infection in brachyceran flies from Iran

Khosravi,

Akbarzadeh,

Karimian

et al. 2024

PLoS ONE

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“…aegypti Significantly decreased 26 , 39 ,currentstudy Female-specific effect 39 ,currentstudy No effect 17 , 38 , 108 Ae. albopictus Significantly increased 63 Haematobia irritans exigua Significantly decreased 109 D. nigrosparsa No effect 110 Fertility a Ae. aegypti Significantly decreased 39 ,currentstudy Female-specific effect 39 ,currentstudy No effect 17 , 108 Bemisia tabaci No effect 111 Longevity D. melanogaster Significant increase in female longevity 106 Temperature and sex dependent 62 Strain dependent 78 Male effect on female longevity 53 Ae.…”

Section: Discussionmentioning

confidence: 99%

wMel Wolbachia alters female post-mating behaviors and physiology in the dengue vector mosquito Aedes aegypti

Osorio,

Villa-Arias,

Camargo

et al. 2023

Commun Biol

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Globally invasive Aedes aegypti disseminate numerous arboviruses that impact human health. One promising method to control Ae. aegypti populations is transinfection with Wolbachia pipientis, which naturally infects ~40–52% of insects but not Ae. aegypti. Transinfection of Ae. aegypti with the wMel Wolbachia strain induces cytoplasmic incompatibility (CI), allows infected individuals to invade native populations, and inhibits transmission of medically relevant arboviruses by females. Female insects undergo post-mating physiological and behavioral changes—referred to as the female post-mating response (PMR)—required for optimal fertility. PMRs are typically elicited by male seminal fluid proteins (SFPs) transferred with sperm during mating but can be modified by other factors, including microbiome composition. Wolbachia has modest effects on Ae. aegypti fertility, but its influence on other PMRs is unknown. Here, we show that Wolbachia influences female fecundity, fertility, and re-mating incidence and significantly extends the longevity of virgin females. Using proteomic methods to examine the seminal proteome of infected males, we found that Wolbachia moderately affects SFP composition. However, we identified 125 paternally transferred Wolbachia proteins, but the CI factor proteins (Cifs) were not among them. Our findings indicate that Wolbachia infection of Ae. aegypti alters female PMRs, potentially influencing control programs that utilize Wolbachia-infected individuals.

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“…In our comparison of Wolbachia density across generations, we considered the relative contributions of genes and environment to the determination of density versus stochastic processes. Temperature and diet have been shown to affect relative Wolbachia densities [ 28 , 29 , 61 , 62 ]. However, a previous study showed that w AlbB remains at a constant density between 26 °C and 37 °C [ 63 ], and under laboratory rearing conditions our temperatures should be largely constant.…”

Section: Discussionmentioning

confidence: 99%

Cross-tissue and generation predictability of relative Wolbachia densities in the mosquito Aedes aegypti

et al. 2022

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Background The insect endosymbiotic bacterium Wolbachia is being deployed in field populations of the mosquito Aedes aegypti for biological control. This microbe prevents the replication of human disease-causing viruses inside the vector, including dengue, Zika and chikungunya. Relative Wolbachia densities may in part predict the strength of this ‘viral blocking’ effect. Additionally, Wolbachia densities may affect the strength of the reproductive manipulations it induces, including cytoplasmic incompatibility (CI), maternal inheritance rates or induced fitness effects in the insect host. High rates of CI and maternal inheritance and low rates of fitness effects are also key to the successful spreading of Wolbachia through vector populations and its successful use in biocontrol. The factors that control Wolbachia densities are not completely understood. Methods We used quantitative PCR-based methods to estimate relative density of the Wolbachia wAlbB strain in both the somatic and reproductive tissues of adult male and female mosquitoes, as well as in eggs. Using correlation analyses, we assessed whether densities in one tissue predict those in others within the same individual, but also across generations. Results We found little relationship among the relative Wolbachia densities of different tissues in the same host. The results also show that there was very little relationship between Wolbachia densities in parents and those in offspring, both in the same and different tissues. The one exception was with ovary–egg relationships, where there was a strong positive association. Relative Wolbachia densities in reproductive tissues were always greater than those in the somatic tissues. Additionally, the densities were consistent in females over their lifetime regardless of tissue, whereas they were generally higher and more variable in males, particularly in the testes. Conclusions Our results indicate that either stochastic processes or local tissue-based physiologies are more likely factors dictating Wolbachia densities in Ae. aegypti individuals, rather than shared embryonic environments or heritable genetic effects of the mosquito genome. These findings have implications for understanding how relative Wolbachia densities may evolve and/or be maintained over the long term in Ae. aegypti. Graphical Abstract

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Transinfection of buffalo flies (Haematobia irritans exigua) with Wolbachia and effect on host biology

Cited by 11 publications

References 77 publications

A survey of Wolbachia infection in brachyceran flies from Iran

A survey of Wolbachia infection in brachyceran flies from Iran

wMel Wolbachia alters female post-mating behaviors and physiology in the dengue vector mosquito Aedes aegypti

Cross-tissue and generation predictability of relative Wolbachia densities in the mosquito Aedes aegypti

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