Transintegumental Glucose Uptake in Schistosomatium douthitti

Cornford, Eain M.; Bocash, William D.; Oldendorf, William H.

doi:10.2307/3280772

Cited by 15 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Km value for glucose transport by S. mansoni appears to be close to 1 mM (reported Km values vary from 0.6 to 2.2 mM). Similarly, the half-saturation constant for glucose uptake in Schistosomatium douthitti (a related species) has been reported to be between 1 and 2 mM (Cornford et al 1981). Our Km value of CsGLUT seems to be well agreed to those of schistosomes.…”

Section: Bsupporting

confidence: 80%

Molecular cloning and functional characterization of a glucose transporter (CsGLUT) in Clonorchis sinensis

Ahn

Cho

et al. 2015

Parasitol Res

View full text Add to dashboard Cite

A complementary DNA (cDNA) encoding a glucose transporter of Clonorchis sinensis (CsGLUT) was isolated from the adult C. sinensis cDNA library. The open reading frame of CsGLUT cDNA consists of 1653 base pairs that encode a 550-amino acid residue protein. Hydropathy analysis suggested that CsGLUT possess 12 putative membrane-spanning domains. The Northern blot analysis result using poly(A)(+)RNA showed a strong band at ~2.1 kb for CsGLUT. When expressed in Xenopus oocytes, CsGLUT mediated the transport of radiolabeled deoxy-D-glucose in a time-dependent but sodium-independent manner. Concentration-dependency results showed saturable kinetics and followed the Michaelis-Menten equation. Nonlinear regression analyses yielded a Km value of 588.5 ± 53.0 μM and a Vmax value of 1500.0 ± 67.5 pmol/oocyte/30 min for [1,2-(3)H]2-deoxy-D-glucose. No trans-uptakes of bile acid (taurocholic acid), amino acids (tryptophan and arginine), or p-aminohippuric acid were observed. CsGLUT-mediated transport of deoxyglucose was significantly and concentration-dependently inhibited by radio-unlabeled deoxyglucose and D-glucose. 3-O-Methylglucose at 10 and 100 μM inhibited deoxyglucose uptake by ~50 % without concentration dependence. No inhibitory effects by galactose, mannose, and fructose were observed. This work may contribute to the molecular biological study of carbohydrate metabolism and new drug development of C. sinensis.

show abstract

Section: Bsupporting

confidence: 80%

Molecular cloning and functional characterization of a glucose transporter (CsGLUT) in Clonorchis sinensis

Ahn

Cho

et al. 2015

Parasitol Res

View full text Add to dashboard Cite

show abstract

“…During pairing, germ cells in the ovary and vitelline gland commence differentiation and produce oocytes or vitellocytes, respectively2. Although a chemical or tactile stimulus from the male is thought to be transferred to the female which triggers a cascade of changes during the pairing process, the stimulating factor(s) or mechanisms associated with male-induced female reproductive development have not been elucidated24567891011121314151617. Understanding the male–female interactions and their effects on reproductive development will provide new insights for the development of novel anti-schistosome strategies.…”

mentioning

confidence: 99%

Dynamic transcriptomes identify biogenic amines and insect-like hormonal regulation for mediating reproduction in Schistosoma japonicum

Wang

Shen

et al. 2017

Nat Commun

View full text Add to dashboard Cite

Eggs produced by the mature female parasite are responsible for the pathogenesis and transmission of schistosomiasis. Female schistosomes rely on a unique male-induced strategy to accomplish reproductive development, a process that is incompletely understood. Here we map detailed transcriptomic profiles of male and female Schistosoma japonicum across eight time points throughout the sexual developmental process from pairing to maturation. The dynamic gene expression pattern data reveal clear sex-related characteristics, indicative of an unambiguous functional division between males and females during their interplay. Cluster analysis, in situ hybridization and RNAi assays indicate that males likely use biogenic amine neurotransmitters through the nervous system to control and maintain pairing with females. In addition, the analyses indicate that reproductive development of females involves an insect-like hormonal regulation. These data sets and analyses serve as a foundation for deeper study of sexual development in this pathogen and identification of novel anti-schistosomal interventions.

show abstract

“…Mammalian blood is a naturally rich medium. Many nutrients in the plasma, such as glucose [ 19 ], fatty acids [ 20 ] and albumin [ 21 ], are utilized by schistosomes. In vivo and in vitro studies have demonstrated that some host nutrients exhibited a stimulating effect on the growth of schistosomes.…”

Section: Introductionmentioning

confidence: 99%

Intake of Erythrocytes Required for Reproductive Development of Female Schistosoma japonicum

Wang

Liu

et al. 2015

PLoS ONE

View full text Add to dashboard Cite

The reproductive development and maturation of female schistosomes are crucial since their released eggs are responsible for the host immunopathology and transmission of schistosomiasis. However, little is known about the nutrients required by female Schistosoma japonicum during its sexual maturation. We evaluated the promoting effect of several nutrients (calf serum, red blood cells (RBCs), ATP and hypoxanthine) on the reproductive development of pre-adult females at 18 days post infection (dpi) from mixed infections and at 50 dpi from unisexual infections of laboratory mice in basic medium RPMI-1640. We found RBCs, rather than other nutrients, promoted the female sexual maturation and egg production with significant morphological changes. In 27% of females (18 dpi) from mixed infections that paired with males in vitro on day 14, vitelline glands could be positively stained by Fast Blue B; and in 35% of females (50 dpi) from unisexual infections on day 21, mature vitelline cells were observed. Infertile eggs were detected among both groups. To analyze which component of mouse RBCs possesses the stimulating effect, RBCs were fractionated and included in media. However, the RBC fractions failed to stimulate development of the female reproductive organs. In addition, bovine hemoglobin hydrolysate, digested by neutral protease, was found to exhibit the promoting activity instead of untreated bovine hemoglobin. The other protein hydrolysate, lactalbumin hydrolysate, exhibited a similar effect with bovine hemoglobin hydrolysate. Using quantitative RT-PCR, we found the expression levels of four reproduction-related genes were significantly stimulated by RBCs. These data indicate that RBCs provide essential nutrients for the sexual maturation of female S. japonicum and that the protein component of RBCs appeared to constitute the key nutrient. These findings would improve laboratory culture of pre-adult schistosomes to adult worms in medium with well-defined components, which is important to investigate the function of genes related to female sexual maturation.

show abstract

Transintegumental Glucose Uptake in Schistosomatium douthitti

Cited by 15 publications

References 0 publications

Molecular cloning and functional characterization of a glucose transporter (CsGLUT) in Clonorchis sinensis

Molecular cloning and functional characterization of a glucose transporter (CsGLUT) in Clonorchis sinensis

Dynamic transcriptomes identify biogenic amines and insect-like hormonal regulation for mediating reproduction in Schistosoma japonicum

Intake of Erythrocytes Required for Reproductive Development of Female Schistosoma japonicum

Contact Info

Product

Resources

About