Transition from blastomere to trophectoderm biopsy: comparing two preimplantation genetic testing for aneuploidies strategies

Coll, Lluc; Parriego, M; Boada, Montserrat; Devesa, Marta; Arroyo, Gemma; Rodríguez, Ignacio; Coroleu, B.; Vidal, Francesca; Veiga, Anna

doi:10.1017/s0967199418000084

Cited by 30 publications

(11 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More recently, cleavage-stage embryo biopsies have been largely replaced by trophectoderm (TE) biopsies. Blastocyst biopsy provides more cells and is performed at an embryonic stage more amenable to genetic analyses, and less sensitive to possible damage (Coll et al, 2018). We lack reports on the incidence of MZT after the use of ART in combination with PGT performed at the blastocyst stage.…”

Section: Introductionmentioning

confidence: 99%

Monozygotic twinning following embryo biopsy at the blastocyst stage

Sellers

Castillo

Ten

et al. 2020

JBRA Assisted Reproduction

View full text Add to dashboard Cite

Objective: Monozygotic twinning incidence following preimplantation genetic testing in embryos at cleavage-stage does not appear to increase; however, data regarding the possible impact of the blastocyst-stage preimplantation genetic testing is lacking. We compared the incidence of monozygotic twinning in preimplantation genetic testing cycles performed at the blastocyst-stage, versus cycles without PGT, following single embryo transfer. Methods: In this retrospective cohort study, we analyzed the incidence of twin pregnancies in patients undergoing intracytoplasmic sperm injection and blastocyst-preimplantation genetic testing (253 cycles), versus a period-matched control population of patients undergoing intracytoplasmic sperm injection and single embryo transfer without preimplantation genetic testing (606 cycles). Results: The overall monozygotic twinning rate was 14/859 (1.6%) per clinical pregnancy. The incidence of zygotic splitting following intracytoplasmic sperm injection and preimplantation genetic testing was 3.5% (95% Confidence interval 1.8%-6.6%) versus 0.8% (95% Confidence interval 0.3%-1.9%) following intracytoplasmic sperm injection without preimplantation sperm injection. After adjusting for potential confounders, preimplantation genetic testing cycles were associated with an increase in the incidence of monozygotic twinning when compared to cycles without embryo biopsy (Odd ratio 3.44, 95% Confidence interval 1.05-11.27, p=0.041). Conclusions: Our findings indicate that embryo biopsy for preimplantation genetic testing performed at the blastocyst stage is associated to an increase in the incidence of monozygotic twinning. Further validation in larger sample size studies is warranted. Patients undergoing preimplantation genetic testing must receive proper counselling about the potential risks of the technique.

show abstract

Section: Introductionmentioning

confidence: 99%

Monozygotic twinning following embryo biopsy at the blastocyst stage

Sellers

Castillo

Ten

et al. 2020

JBRA Assisted Reproduction

View full text Add to dashboard Cite

show abstract

“…In another study, transferred embryos in both groups were at blastocyst stage, however, bias remained since fresh ET was performed after CB but a frozen-warmed ET was administered after BB. No significant differences in implantation rate (50.3% fresh blastocyst transfer after CB vs. 61.4% FWBT after BB) and clinical pregnancy rate (56.1% vs. 65.3% in two groups respectively) were found between the two groups in the study (17).…”

Section: Discussionmentioning

confidence: 67%

“…However, the results were controversial. Some trials reported a more compromised implantation potential of biopsied embryos and an inferior clinical pregnancy rate per cycle after CB was performed (6,14,15), while others found no statistically significant differences between CB and BB (16,17). It is worth noting that bias may exist in previous studies e.g.…”

Section: Introductionmentioning

confidence: 99%

Impact of embryo biopsy stage on implantation potential in thalassemias: a propensity score matching study

Wang¹,

Ding²,

Lu³

et al. 2019

Preprint

View full text Add to dashboard Cite

Objective: To investigate the impact of biopsy stage on implantation potential of frozen-warmed blastocysts in pre-implantation genetic testing (PGT) cycles for those with thalassemias.Design: Retrospective paired cohort study using propensity score matching (PSM). A time-to-event analysis of Fine-Gray's analysis was used to compare cumulative live birth rate (CLBR). Setting: Single university-affiliated center . Patient(s): A total of 439 PGT couples were included who underwent PGT for thalassemias between 2015 and 2017. Intervention(s): Two biopsy strategies were evaluated; cleavage-stage biopsy (CB) and blastocyststage biopsy (BB). Main Outcome Measure(s): Implantation rate and CLBR. Result(s): A total of 202 ovum pick-up (OPU) cycles from Group CB and 237 OPU cycles from Group BB were included. PSM was conducted for two groups covering characteristics on the OPU cycle and the first frozen-warmed blastocyst transfer (FWBT) cycle , resulting in 140 matched pairs. The implantation rate was significantly lower in the CB group (41.0%, 30.3%, 20.4% in 1st, 2nd and 3rdFWBT cycle respectively, weighted mean 34.2%) than those in the BB group (51.8%, 43.5%, and 40.5% (weighted mean 48.1%), respectively; at P = 0.044, 0.047, 0.039). This was a 28.9% relative reduction ( P = 0.002). The CLBR across three FWBT cycles was significantly higher in the BB group compared with the CB group (80.0% vs. 62.1%, P = 0.032), while the likelihood of LBR was 1.21-fold higher after regression adjustment. Conclusion(s):Cleavage-stage biopsy may partially affect the implantation potential and CLBRs of frozen-warmed blastocysts, compared with blastocyst stage biopsy.

show abstract

“…Gamete manipulation, oocyte insemination, and embryo culture were performed according to standard protocols used in the IVF laboratory of the study's center [19].…”

Section: Embryo Manipulation and Pgt-amentioning

confidence: 99%

What is the clinical impact of the endometrial receptivity array in PGT-A and oocyte donation cycles?

Neves

Devesa

Martínez

et al. 2019

J Assist Reprod Genet

Self Cite

View full text Add to dashboard Cite

Purpose To evaluate the influence of the endometrial receptivity array (ERA) test on the implantation rate (IR) and pregnancy rate (PR) in patients with previous failed euploid embryo transfers (Euploid-ET) or oocyte donation embryo transfers (Donor-ET). Methods Single-center retrospective study of patients with ≥ 1 previous failed Euploi-ET (n = 24) or ≥ 2 failed Donor-ET (n = 32) who underwent an ERA test and a post-ERA Euploid-ET/Donor-ET between 2012 and 2018. Controls were patients with ≥ 1 previously failed Euploid-ET (n = 119) or ≥ 2 failed Donor-ET (n = 158) who underwent Euploid-ET/Donor-ET during the same period without performing an ERA test. Only blastocyst stage embryos were included. IR/PR was compared between the post-ERA ET and the last ET in the control group. Results There was no statistically significant difference regarding IR [55.6% (34.6-76.5%) vs. 65.0% (56.9-73.1%)] nor PR (58.3% vs.70.6%, p = 0.238) in the Euploid-ET ERA vs. Euploid-ET control groups. In the Donor-ET arm, both IR [26.8% (12.3-41.4%) vs. 57.2% (50.1-64.3%)] and PR (34.4% vs. 65.2%, p = 0.001) were significantly lower in the ERA group. Multivariate analysis confirmed that performing an ERA test did not influence the PR in the Euploid-ET arm and was associated with a diminished PR in the Donor-ET arm. In the ERA group, 41.1% patients were non-receptive (NR). No significant difference was found regarding IR/PR in NR vs. receptive patients in both Euploid-ET/Donor-ET arms. Conclusions In our sample, the performance of an ERA test did not improve pregnancy outcomes. Future prospective studies in larger samples are needed to confirm the role of the ERA test in Euploid-ET/Donor-ET.

show abstract

Transition from blastomere to trophectoderm biopsy: comparing two preimplantation genetic testing for aneuploidies strategies

Cited by 30 publications

References 42 publications

Monozygotic twinning following embryo biopsy at the blastocyst stage

Monozygotic twinning following embryo biopsy at the blastocyst stage

Impact of embryo biopsy stage on implantation potential in thalassemias: a propensity score matching study

What is the clinical impact of the endometrial receptivity array in PGT-A and oocyte donation cycles?

Contact Info

Product

Resources

About