Transitory improvement of articular cartilage characteristics after implantation of polylactide:polyglycolic acid (PLGA) scaffolds seeded with autologous mesenchymal stromal cells in a sheep model of critical-sized chondral defect

Caminal, Marta; Moll, Xavier; Codina, David; Rabanal, Rosa M.; Morist, A.; Barrachina, J.; García, Félix; Pla, Arnau; Vives, Joaquim

doi:10.1007/s10529-014-1585-3

Cited by 22 publications

(11 citation statements)

References 31 publications

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“…of studies using the score (%)ReferencesHistology scores111 (100%)[49, 54–102, 134, 150–154, 160, 161, 272–324]International Cartilage Repair Society Score26 (23%)[49, 60, 61, 63, 66, 69, 72, 74, 79, 89, 92, 94, 98, 99, 272, 282, 283, 289, 305, 306, 310, 313, 314, 316, 319, 324]Wakitani score21 (19%)[58, 62, 67, 68, 72, 73, 80, 82, 97, 151, 273, 277, 279, 284, 285, 290, 299, 304, 310, 321]O’Driscoll score2018%[49, 71, 81, 84, 85, 93, 100, 160, 272, 276, 290, 296–298, 302, 306, 308, 313, 314, 322]Functional scores/mechanical11 (10%)[55, 57, 62, 67, 69, …”

Section: Resultsmentioning

confidence: 99%

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

et al. 2017

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BackgroundThe management of articular cartilage defects presents many clinical challenges due to its avascular, aneural and alymphatic nature. Bone marrow stimulation techniques, such as microfracture, are the most frequently used method in clinical practice however the resulting mixed fibrocartilage tissue which is inferior to native hyaline cartilage. Other methods have shown promise but are far from perfect. There is an unmet need and growing interest in regenerative medicine and tissue engineering to improve the outcome for patients requiring cartilage repair. Many published reviews on cartilage repair only list human clinical trials, underestimating the wealth of basic sciences and animal studies that are precursors to future research. We therefore set out to perform a systematic review of the literature to assess the translation of stem cell therapy to explore what research had been carried out at each of the stages of translation from bench-top (in vitro), animal (pre-clinical) and human studies (clinical) and assemble an evidence-based cascade for the responsible introduction of stem cell therapy for cartilage defects.Main body of abstractThis review was conducted in accordance to PRISMA guidelines using CINHAL, MEDLINE, EMBASE, Scopus and Web of Knowledge databases from 1st January 1900 to 30th June 2015. In total, there were 2880 studies identified of which 252 studies were included for analysis (100 articles for in vitro studies, 111 studies for animal studies; and 31 studies for human studies). There was a huge variance in cell source in pre-clinical studies both of terms of animal used, location of harvest (fat, marrow, blood or synovium) and allogeneicity. The use of scaffolds, growth factors, number of cell passages and number of cells used was hugely heterogeneous.Short conclusionsThis review offers a comprehensive assessment of the evidence behind the translation of basic science to the clinical practice of cartilage repair. It has revealed a lack of connectivity between the in vitro, pre-clinical and human data and a patchwork quilt of synergistic evidence. Drivers for progress in this space are largely driven by patient demand, surgeon inquisition and a regulatory framework that is learning at the same pace as new developments take place.

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Section: Resultsmentioning

confidence: 99%

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

et al. 2017

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“…This is not only because of the multilineage progenitor potential of MSCs, but also because of their capacity to modulate the host microenvironment by secreting a variety of factors, such as cytokines, chemokines or growth factors, which can mediate diverse functions via crosstalk between different cell types (Friedenstein et al, ; Doorn et al, ; Murphy et al, ). Examples of using sheep as a model system include spinal fusion (Goldschlager et al, ), segmental tibial defects (Niemeyer et al, ), chondral/osteochondral repair (Ahern et al, ; Caminal et al, ,b, ; Vives et al, ), tendon and ligament regeneration (Lacitignola et al, ) and osteonecrosis of the femoral head (Velez et al, ), among others.…”

Section: Introductionmentioning

confidence: 99%

A reproducible method for the isolation and expansion of ovine mesenchymal stromal cells from bone marrow for use in regenerative medicine preclinical studies

Caminal

Vélez

Rabanal

et al. 2016

J Tissue Eng Regen Med

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The use of multipotent mesenchymal stromal cells (MSCs) as candidate medicines for treating a variety of pathologies is based on their qualities as either progenitors for the regeneration of damaged tissue or producers of a number of molecules with pharmacological properties. Preclinical product development programmes include the use of well characterized cell populations for proof of efficacy and safety studies before testing in humans. In the field of orthopaedics, an increasing number of translational studies use sheep as an in vivo test system because of the similarities with humans in size and musculoskeletal architecture. However, robust and reproducible methods for the isolation, expansion, manipulation and characterization of ovine MSCs have not yet been standardised. The present study describes a method for isolation and expansion of fibroblastic-like, adherent ovine MSCs that express CD44, CD90, CD140a, CD105 and CD166, and display trilineage differentiation potential. The 3-week bioprocess proposed here typically yielded cell densities of 1.4 × 10 MSCs/cm at passage 2, with an expansion factor of 37.8 and approximately eight cumulative population doublings. The osteogenic potential of MSCs derived following this methodology was further evaluated in vivo in a translational model of osteonecrosis of the femoral head, in which the persistence of grafted cells in the host tissue and their lineage commitment into osteoblasts and osteocytes was demonstrated by tracking enhanced green fluorescent protein-labelled cells. Copyright © 2016 John Wiley & Sons, Ltd.

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“…Previous data in large translational animal models [ 15 ], and investigations on the role of MSC in cartilage [ 16 , 17 ] and bone [ 18 ] regeneration, provided further support with respect to the safety and regenerative qualities of MSC.…”

Section: Resultsmentioning

confidence: 91%

Development of an advanced cell therapy product indicated for the treatment of gonarthrosis

Vives¹,

Blanco²,

Caminal³

et al. 2015

BMC Proc

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Transitory improvement of articular cartilage characteristics after implantation of polylactide:polyglycolic acid (PLGA) scaffolds seeded with autologous mesenchymal stromal cells in a sheep model of critical-sized chondral defect

Cited by 22 publications

References 31 publications

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

A reproducible method for the isolation and expansion of ovine mesenchymal stromal cells from bone marrow for use in regenerative medicine preclinical studies

Development of an advanced cell therapy product indicated for the treatment of gonarthrosis

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