Translation of the four major RNA species of cucumber mosaic virus in plant and animal cell-free systems and in toad oocytes

Schwinghamer, Mark W.; Symons, Robert H.

doi:10.1016/0042-6822(77)90337-3

Cited by 84 publications

(32 citation statements)

References 57 publications

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“…Some preparations of RNA were extracted from particles after only a single cycle of density gradient fractionation, and further purified either by electrophoresis in, and elution from, 2.2% polyacrylamide slab gels (Schwinghamer & Symons, 1977) or by sedimentation (16 h at 23 000 rev/min in a Beckman SW27 rotor) through a 10 to 40% sucrose density gradient in 20 mM-Tris-HCl pH 8-5 + 0.1 M-NaC1 + 1 mM-EDTA.…”

Section: Introductionmentioning

confidence: 99%

RNA Species of Tobacco Rattle Virus Strains and Their Nucleotide Sequence Relationships

Robinson¹

1983

Journal of General Virology

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SUMMARYThe nucleotide sequences of the genome RNA species (RNA-1 and RNA-2) of three strains of tobacco rattle virus OR, SYM and CAM, were compared by hybridization with complementary DNA copies. Strain OR was found to contain two RNA-2 species, which differ by about 5 × 104 in mol. wt. (150 nucleotides), but about 90~ of whose nucleotide sequences are homologous. The larger of the two contained the genetic information for yellow symptoms in infected Nicotiana spp., whereas the smaller, which probably arose from it by mutation, did not. The RNA-2 species of strains OR, SYM and CAM had no detectable nucleotide sequences in common. RNA-1 of strain OR and of strain SYM were almost identical in sequence, but had no detectable sequence in common with RNA-1 of strain CAM. Only in strain CAM was any nucleotide sequence homology between RNA-1 and RNA-2 detected, amounting to about 450 residues.

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Section: Introductionmentioning

confidence: 99%

RNA Species of Tobacco Rattle Virus Strains and Their Nucleotide Sequence Relationships

Robinson¹

1983

Journal of General Virology

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“…The subgenomic RNA 4 is derived from RNA 3 during the infection cycle [3] and is located at the 3' end of RNA 3 [4]. Therefore, the location of the 5' terminus of RNA 4 within RNA 3 was determined by direct enzymatic sequencing [19,25] of 5'-32P-labelled RNA 4 (J. Haseloff, unpublished data).…”

Section: Cornplere Nucleotide Sequencf Of CMV R N a 3 And R N Amentioning

confidence: 99%

“…Only RNAs 1, 2 and 3 are required for infectivity [I] with the coat protein being genetically specified by RNA 3 [2,3]. However, translation of RNA 3 in vitro gives a protein product of M , 35000 (3A protein) while RNA 4 codes for the synthesis of coat protein ( M , 24500) and is considered to be the subgenomic mRNA for coat protein in viva [3]. RNA 3, therefore, has the coding potential for two proteins although the function of the 35000-Mr protein is unknown.…”

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confidence: 99%

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Cucumber Mosaic Virus RNA 3

Gould

Symons

1982

European Journal of Biochemistry

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The complete sequence of the 2193 residues of RNA 3 ( M , 746000) of cucumber mosaic virus (Q strain) was determined by a combination of chemical and enzymic sequencing techniques utilizing cloned DNA fragments. The nucleotide sequence of RNA 3 also gave the complete sequence of 1027 residues of RNA 4 ( M , 349000), which codes for the viral coat protein and is derived from the 3' end; there are 53 untranslated nucleotides at the 5' end of RNA 4. The nucleotide sequence provided the amino acid sequences of the two proteins coded for by RNA 3 : the 5'-terminal 3A protein with 333 amino acids ( M , 36 700) and the 3'-terminal viral coat protein with 236 amino acids ( M , 26200). These two coding regions are in the same reading frame, are separated by an intercistronic region of 123 nucleotides and are flanked by two untranslated regions of 94 nucleotides at the 5' terminus and of 263 nucleotides at the 3' terminus.Secondary structure models are postulated for parts of the RNA 3 sequence. These are considered to be important in the control of the translation and replication of RNA 3 and in the processing of RNA 3 to give RNA 4.Cucumber mosaic virus (CMV) is a single-stranded (+) RNA plant virus with a functionally divided genome. Purified virus preparations contain four RNA species, designated RNAs 1-4 in order of decreasing molecular weight ( M ,

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“…Its genome consists of three functional single-stranded RNA molecules designated as RNA 1, 2, and 3, which are required for infection. RNA 1 and 2 encode components of the viral RNA-dependent RNA polymerase (Nitta et al, 1988), while RNA 3 encodes the movement protein (MP) (Suzuki et al, 1991) and coat protein (CP) (Schwinghamer and Symons, 1977). The CP is translated from the subgenomic RNA 4 which is encoded by the 3'-half of the RNA 3 (Gould and Symons, 1982 overlapping gene (2b), encoded by RNA 2, was discovered and is most likely expressed through subgenomic RNA (Ding et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Alstroemeria-Infecting Cucumber Mosaic Virus Isolates Contain Additional Sequences in the Rna 3 Segment

Chen¹,

Prins²,

Derks³

et al. 2002

Acta Hortic.

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The coat protein (CP) genes and flanking regions of three alstroemeriainfecting cucumber mosaic virus isolates (CMV-ALS), denoted ALS-LBO, ALS-IPO, and ALS-NAK, were cloned and their nucleotide sequence determined and compared at both nucleic acid and deduced protein level with the published sequences of CMV RNA 3. The sequences of these isolates showed more than 95% nucleotide and peptide sequence homology to each other and to other members of subgroup II CMV. Strikingly, an additional sequence of 218 nt was found in the central region of the 3'-non-translated region (NTR) of RNA 3 of two out of three isolates. The additional sequence appeared to have arisen from RNAs 1 or 2. A subgroup-specific DIG-labeled probe has been developed from this additional sequence and applied to detect subgroup II CMV strains in dot blot hybridization and to differentiate the Alstroemeria isolates containing additional sequences in Northern blot hybridization.

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Translation of the four major RNA species of cucumber mosaic virus in plant and animal cell-free systems and in toad oocytes

Cited by 84 publications

References 57 publications

RNA Species of Tobacco Rattle Virus Strains and Their Nucleotide Sequence Relationships

RNA Species of Tobacco Rattle Virus Strains and Their Nucleotide Sequence Relationships

Cucumber Mosaic Virus RNA 3

Alstroemeria-Infecting Cucumber Mosaic Virus Isolates Contain Additional Sequences in the Rna 3 Segment

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