Translation of the long-term fundamental studies on viral DNA packaging motors into nanotechnology and nanomedicine

Liang, Chenxi; Weitao, Tao; Zhou, Lixia; Guo, Peixuan

doi:10.1007/s11427-020-1752-1

Cited by 5 publications

(4 citation statements)

References 287 publications

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“…A key characteristic of “living beings” is movement. These motion activities are usually performed by ATPase motors [13] , [14] . One of the most important motor functions is dsDNA translocation, which occurs during DNA replication, cellular mitosis, plasmid conjugation, DNA repair, and dsDNA viral genome packaging.…”

Section: Research Highlightsmentioning

confidence: 99%

Biomotors, viral assembly, and RNA nanobiotechnology: Current achievements and future directions

Rolband

Beasock

Wang

et al. 2022

Computational and Structural Biotechnology Journal

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Section: Research Highlightsmentioning

confidence: 99%

Biomotors, viral assembly, and RNA nanobiotechnology: Current achievements and future directions

Rolband

Beasock

Wang

et al. 2022

Computational and Structural Biotechnology Journal

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“…As an emerging nanotechnology, a DNA‐fueled molecular machine can respond to external signal stimulation and achieve information transmission and signal amplification by mechanical nano movement at the micro‐ or nanoscale, showing great application potential as a new sensor. In recent years, various DNA‐fueled molecular machines, including DNA tweezers (Landon et al., 2012), walkers (Pan et al., 2015; Song et al., 2022; Yang et al., 2022), and motors (Bazrafshan et al., 2021; Liang et al., 2020), have been developed for sensor research. Under specific conditions, different molecular machines can rapidly detect target objects.…”

Section: Introductionmentioning

confidence: 99%

Application of DNA‐fueled molecular machines in food safety testing

Ma,

Chen,

Zhang

et al. 2024

Comp Rev Food Sci Food Safe

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Food is consumed by humans, which is indispensable to human life. Therefore, considerable attention of the whole society has been paid to food safety. Over the last few years, dramatic social development has brought new challenges to food safety, making developing new and quick methods for on‐site food safety testing an important necessity. As a result, DNA‐fueled molecular machines, characterized by high efficiency, accuracy, and sensitivity in testing, have come into the spotlight, based on which sensors can be constructed to detect toxic and harmful substances in food products. This study reviewed recent research on several DNA‐fueled molecular machines, including DNA tweezers, DNA walkers, and DNA origami, for rapidly detecting toxic and harmful substances. Based on the above studies, the sensitivity and timeliness of several DNA molecular machines were summarized and compared, and the development prospect of DNA fuel molecular machines in the field of food safety detection was prospected.

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“…These motors are powerful enough to package DNA against an extremely high internal pressure (tens of atmospheres) established by densely packed negatively charged DNA inside the shell. As such, there have been extensive efforts in engineering viral packaging motors as a functional nanodevice for the sensing of DNA, RNA, peptides, and chemicals . Among various motors, bacteriophage ϕ29 has been developed into a highly efficient in vitro system and therefore served as an excellent model system for mechanistic studies of biomotors and nanotechnological development, including the diagnosis of disease. , …”

mentioning

confidence: 99%

“…As such, there have been extensive efforts in engineering viral packaging motors as a functional nanodevice for the sensing of DNA, RNA, peptides, and chemicals. 3 Among various motors, bacteriophage ϕ29 has been developed into a highly efficient in vitro system and therefore served as an excellent model system for mechanistic studies of biomotors 4 and nanotechnological development, including the diagnosis of disease. 5,6 Viral packaging motors can be classified into two distinct families: the terminase family and the HerA/FtsK-type ATPases.…”

mentioning

confidence: 99%

Structural Insights into gp16 ATPase in the Bacteriophage ϕ29 DNA Packaging Motor

et al. 2021

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Biological motors, ubiquitous in living systems, convert chemical energy into different kinds of mechanical motions critical to cellular functions. Most of these biomotors belong to a group of enzymes known as ATPases, which adopt a multi-subunit ring-shaped structure and hydrolyze adenosine triphosphate (ATP) to generate forces. The gene product 16 (gp16), an ATPase in bacteriophage 29, is among the most powerful biomotors known. It can overcome substantial resisting forces from entropic, electrostatic, and DNA bending sources to package double-stranded DNA (dsDNA) into a preformed protein shell (procapsid). Despite numerous studies of the 29 packaging mechanism, a structure of the full-length gp16 is still lacking, let alone that of the packaging motor complex that includes two additional molecular components: a connector gp10 protein and a prohead RNA (pRNA).Here we report the crystal structure of the C-terminal domain of gp16 (gp16-CTD). Structure-based alignment of gp16-CTD with related RNase H-like nuclease domains revealed a nucleic acid binding surface in gp16-CTD, whereas no nuclease activity has been detected for gp16. Subsequent molecular dynamics (MD) simulations showed that this nucleic acid binding surface is likely essential for pRNA binding. Furthermore, our simulations of a full-length gp16 structural model highlighted a dynamic interplay between the N-terminal domain (NTD) and CTD of gp16, which may play a role in driving DNA movement into the procapsid, providing structural support to the previously proposed inchworm model. Lastly, we assembled an atomic structural model of the complete 29 dsDNA packaging motor complex by integrating structural and experimental data from multiple sources. Collectively, our findings provided a refined inchworm-revolution model for dsDNA translocation in bacteriophage 29 and suggested how the individual domains of gp16 work together to power such translocation..

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Translation of the long-term fundamental studies on viral DNA packaging motors into nanotechnology and nanomedicine

Cited by 5 publications

References 287 publications

Biomotors, viral assembly, and RNA nanobiotechnology: Current achievements and future directions

Biomotors, viral assembly, and RNA nanobiotechnology: Current achievements and future directions

Application of DNA‐fueled molecular machines in food safety testing

Structural Insights into gp16 ATPase in the Bacteriophage ϕ29 DNA Packaging Motor

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