Translational biotechnology for the control of ticks and tick-borne diseases

Fuente, José de la

doi:10.1016/j.ttbdis.2021.101738

Cited by 21 publications

(19 citation statements)

References 41 publications

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“…This platform focuses on the characterization of host-pathogen molecular interactions using a systems biology approach for the analysis of omics datasets [70]. A proposed pipeline for quantum vaccinomics is based on the characterization of the cell interactome and regulome in vector-host-pathogen interactions and the identification of protective epitopes in protein interacting domains for the design and production of chimeric vaccine antigens [68,69,71,72]. This platform also provides the possibility of including other biomolecules, such as glycan alpha-gal (Galα1-3Galβ1-(3)4GlcNAc-R)-based protein posttranslational modifications, to improve protective immunity against SARS-CoV-2 and other pathogens [73][74][75][76].…”

Section: Discussionmentioning

confidence: 99%

Antibody isotype epitope mapping of SARS‐CoV‐2 Spike RBD protein: Targets for COVID‐19 symptomatology and disease control

et al. 2023

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Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) still poses a challenge for biomedicine and public health. To advance the development of effective diagnostic, prognostic, and preventive interventions, our study focused on high‐throughput antibody binding epitope mapping of the SARS‐CoV‐2 spike RBD protein by IgA, IgM and IgG antibodies in saliva and sera of different cohorts from healthy uninfected individuals to SARS‐CoV‐2‐infected unvaccinated and vaccinated asymptomatic, recovered, nonsevere, and severe patients. Identified candidate diagnostic (455‐LFRKSNLKPFERD‐467), prognostic (395‐VYADSFVIRGDEV‐407‐C‐KLH, 332‐ITNLCPFGEV‐342‐C‐KLH, 352‐AWNRKRI‐358‐C‐KLH, 524‐VCGPKKSTNLVKN‐536‐KLH), and protective (MKLLE‐487‐NCYFPLQSYGFQPTNGVG‐504‐GGGGS‐446‐GGNYNYLYRLFRKSNLKPFERD‐467) epitopes were validated with sera from prevaccine and postvaccine cohorts. The results identified neutralizing epitopes and support that antibody recognition of linear B‐cell epitopes in RBD protein is associated with antibody isotype and disease symptomatology. The findings in asymptomatic individuals suggest a role for anti‐RBD antibodies in the protective response against SARS‐CoV‐2. The possibility of translating results into diagnostic interventions for the early diagnosis of asymptomatic individuals and prognosis of disease severity provides new tools for COVID‐19 surveillance and evaluation of risks in hospitalized patients. These results, together with other approaches, may contribute to the development of new vaccines for the control of COVID‐19 and other coronavirus‐related diseases using a quantum vaccinomics approach through the combination of protective epitopes.

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Section: Discussionmentioning

confidence: 99%

Antibody isotype epitope mapping of SARS‐CoV‐2 Spike RBD protein: Targets for COVID‐19 symptomatology and disease control

et al. 2023

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“…Developing chemicals that can interfere with the phase transition of tick-GRP77 and thus inhibit cement cone formation may provide an effective solution. Anti-tick vaccines are a promising tick control strategy with the potential to provide long-term protection 77, 78, 79 . Characterization of GRPs have shown they are immunogenic, play a role in host’s immune system evasion, and are required for successful tick attachment, making them promising anti-tick candidates 80, 81, 82 .…”

Section: Discussionmentioning

confidence: 99%

Phase Separation and Ageing of Glycine-Rich Protein from Tick Adhesive

Ganar

Turbina

Nandy

et al. 2023

Preprint

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Hard ticks feed on their host for multiple days. To ensure firm attachment, they secrete a protein-rich saliva that eventually forms a solid cement cone. The underlying mechanism of this liquid-to-solid transition is not yet understood. This study focuses on the phase transitions of a disordered glycine-rich protein (GRP) that is prominent in tick saliva. We show that GRP undergoes liquid-liquid phase separation via simple coacervation to form biomolecular condensates in salty environments. Cation-pi and pi-pi interactions near the C-terminus promote coacervation while a negatively charged N-terminus prolongs its onset through electrostatic repulsion. Interestingly, GRP condensates exhibit ageing and undergo liquid-to-gel transition to form viscoelastic networks as well as solid-like condensates. Lastly, we provide evidence for protein-rich condensates in natural tick saliva. Our findings provide a starting point to gain insights into the bioadhesion of ticks, develop novel tick control strategies, and towards biomedical applications such as tissue sealants.

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“…With the emergence of high throughput analysis, several studies have provided enormous amount of information on proteins present in saliva and salivary glands that are important in tick feeding and/or pathogen interactions ( Artigas-Jeronimo et al., 2018 ; De La Fuente, 2021 ; Martins et al., 2021 ; Oleaga et al., 2021 ). It is a daunting task to annotate several of the arthropod proteins due to lack of genome information for many tick species.…”

Section: Future Perspectivesmentioning

confidence: 99%

Tick Saliva and Salivary Glands: What Do We Know So Far on Their Role in Arthropod Blood Feeding and Pathogen Transmission

Neelakanta

Sultana

2022

Front. Cell. Infect. Microbiol.

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Ticks are blood-sucking arthropods that have developed myriad of strategies to get a blood meal from the vertebrate host. They first attach to the host skin, select a bite site for a blood meal, create a feeding niche at the bite site, secrete plethora of molecules in its saliva and then starts feeding. On the other side, host defenses will try to counter-attack and stop tick feeding at the bite site. In this constant battle between ticks and the host, arthropods successfully pacify the host and completes a blood meal and then replete after full engorgement. In this review, we discuss some of the known and emerging roles for arthropod components such as cement, salivary proteins, lipocalins, HSP70s, OATPs, and extracellular vesicles/exosomes in facilitating successful blood feeding from ticks. In addition, we discuss how tick-borne pathogens modulate(s) these components to infect the vertebrate host. Understanding the biology of arthropod blood feeding and molecular interactions at the tick-host interface during pathogen transmission is very important. This information would eventually lead us in the identification of candidates for the development of transmission-blocking vaccines to prevent diseases caused by medically important vector-borne pathogens.

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Translational biotechnology for the control of ticks and tick-borne diseases

Cited by 21 publications

References 41 publications

Antibody isotype epitope mapping of SARS‐CoV‐2 Spike RBD protein: Targets for COVID‐19 symptomatology and disease control

Antibody isotype epitope mapping of SARS‐CoV‐2 Spike RBD protein: Targets for COVID‐19 symptomatology and disease control

Phase Separation and Ageing of Glycine-Rich Protein from Tick Adhesive

Tick Saliva and Salivary Glands: What Do We Know So Far on Their Role in Arthropod Blood Feeding and Pathogen Transmission

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