Translational control of the expression of the β subunit gene of E., coli RNA polymerase

Peacock, Susan; Brot, Nathan; Weissbach, Herbert

doi:10.1016/0006-291x(83)91100-2

Cited by 8 publications

(4 citation statements)

References 13 publications

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“…The addition of 0.5 ,ug of X repressor to the incubations completely inhibited the formation of fMet-Asp, fMet-Val, and fMet-Thr, confirming that their synthesis is under the regulation of the phage A PL promoter. In control experimetits, the A repressor had no effect on the synthesis offMetSer or fMet-Val directed by plasmid pNF1337 (16,18), which carries the E. coli L10 operon (data not shown). The following results indicate that IHF increased the amount of translatable cII mRNA synthesized.…”

Section: Resultsmentioning

confidence: 99%

“…Further experiments in which transcription was uncoupled from translation were performed to verify that IHF functions during transcription (16). In these experiments, IHF was added either at the beginning of transcription or at the beginning of translation-i.e., when DNase or rifampicin was added.…”

Section: Resultsmentioning

confidence: 99%

“…and phage X cI repressor was the gift of C. Pabo (Johns Hopkins University). IHF was purified as described (5) thesis have been described (16,18,19 The in vitro synthesized protein products were separated by electrophoresis on 15% NaDodSO4/polyacrylamide gels (22). The gels were dried and fluorographed at -70°C by using Enlightning (New England Nuclear).…”

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confidence: 99%

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In vitro regulation of phage lambda cII gene expression by Escherichia coli integration host factor.

Peacock¹,

Weissbach²,

Nash³

1984

Proc. Natl. Acad. Sci. U.S.A.

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The effect of Escherichia coli integration host factor (IHF) on phage A gene expression has been examined in a simplified DNA-directed in vitro system that measures the formation of the first dipeptide of the gene product. Plasmid pKC3OcII, which contains the phage A genes N, ciH and 0, under control of the PL promoter, was used as template to study the expression of the first dipeptide of the gene products-i.e., fMet-Asp for N protein, fMet-Val for cII, and fMet-Thr for 0. Purified IHF stimulates the DNA-directed synthesis of fMet-Val (cdi) and fMet-Thr (0) 2-3-fold but has no effect on the synthesis of fMet-Asp (N). In this in vitro system, the stimulation by IHF of cII and 0 gene expression is at the level of transcription. Phage A repressor completely inhibits dipeptide synthesis in the presence or absence of IHF. The results are consistent with a role of IHF as a transcription antiterminator, perhaps functioning at or near the tRl site preceding the cII gene.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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In vitro regulation of phage lambda cII gene expression by Escherichia coli integration host factor.

Peacock¹,

Weissbach²,

Nash³

1984

Proc. Natl. Acad. Sci. U.S.A.

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“…The The preparation and characterization of the purified metJ gene product will be described elsewhere. The (20).…”

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confidence: 99%

Regulation of methionine synthesis in Escherichia coli : Effect of metJ gene product and S -adenosylmethionine on the expression of the metF gene

Shoeman

Redfield

Coleman

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

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The regulation of the expression of the Escherichia coli metF gene, which codes for 5,10-methylenetetrahydrofolate reductase (EC 1.1.99.15), has been investigated by using a simplified DNA-directed in vitro system that measures the formation of the first dipeptide (fMet-Ser) of the gene product. The synthesis of fMet-Ser directed by a plasmid containing the metF gene is specifically inhibited by metJ protein (repressor protein). S-Adenosylmethionine enhances the inhibition by the metJ protein of metF gene expression. The inhibition by the metJ protein is at the level of transcription and the results suggest that S-adenosylmethionine is functioning as an allosteric effector.

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Regulation of the Escherichia coli L10 Operon

Brot¹,

Peacock²,

Weissbach³

1986

Springer Series in Molecular Biology

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Translational control of the expression of the β subunit gene of E., coli RNA polymerase

Cited by 8 publications

References 13 publications

In vitro regulation of phage lambda cII gene expression by Escherichia coli integration host factor.

In vitro regulation of phage lambda cII gene expression by Escherichia coli integration host factor.

Regulation of methionine synthesis in Escherichia coli : Effect of metJ gene product and S -adenosylmethionine on the expression of the metF gene

Regulation of the Escherichia coli L10 Operon

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