Translational Coupling Controls Expression and Function of the DrrAB Drug Efflux Pump

Pradhan, Prajakta; Li, Wen; Kaur, Parjit

doi:10.1016/j.jmb.2008.11.027

Cited by 39 publications

(27 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The use of a brief induction makes it unlikely that translation features have been altered artificially by overproduction (Results). However, we anticipate that, to study translation of multisubunit proteins, it may be necessary to coexpress multiple cistrons in the cis configuration (58). SecA is physiologically translated from secM-secA mRNA, in which the upstream secM could affect the folding behavior of the nascent SecA chain (59); therefore it may be necessary to express secA in the presence of cis-expressed secM to study the secA translational pausing further.…”

Section: Discussionmentioning

confidence: 99%

Integrated in vivo and in vitro nascent chain profiling reveals widespread translational pausing

Chadani

Niwa

Chiba

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Although the importance of the nonuniform progression of elongation in translation is well recognized, there have been few attempts to explore this process by directly profiling nascent polypeptides, the relevant intermediates of translation. Such approaches will be essential to complement other approaches, including ribosome profiling, which is extremely powerful but indirect with respect to the actual translation processes. Here, we use the nascent polypeptide's chemical trait of having a covalently attached tRNA moiety to detect translation intermediates. In a case study, Escherichia coli SecA was shown to undergo nascent polypeptide-dependent translational pauses. We then carried out integrated in vivo and in vitro nascent chain profiling (iNP) to characterize 1,038 proteome members of E. coli that were encoded by the first quarter of the chromosome with respect to their propensities to accumulate polypeptidyl-tRNA intermediates. A majority of them indeed undergo single or multiple pauses, some occurring only in vitro, some occurring only in vivo, and some occurring both in vivo and in vitro. Thus, translational pausing can be intrinsically robust, subject to in vivo alleviation, or require in vivo reinforcement. Cytosolic and membrane proteins tend to experience different classes of pauses; membrane proteins often pause multiple times in vivo. We also note that the solubility of cytosolic proteins correlates with certain categories of pausing. Translational pausing is widespread and diverse in nature.translation pausing | nascent polypeptides j Escherichia coli | peptidyl-tRNA

show abstract

Section: Discussionmentioning

confidence: 99%

Integrated in vivo and in vitro nascent chain profiling reveals widespread translational pausing

Chadani

Niwa

Chiba

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Ketoacyl reductase in FAS was one of the reacted sites that TFS attacked on. In addition, TFS significantly reduced food intake, body weight and FAS activities in livers of diet-induced obesity SD rats and mice via oral administration (41,42). Other bioactive traditional Chinese medicine are Alpinia officinarum Hance, Licorice, Polygonum multiflorum, Parasitic loranthus, Ginkgo leaf, Chrysanthemum, Cerasus humilis, and Folium cortex eucommiae.…”

Section: Fatty Acid Synthetase Inhibitormentioning

confidence: 98%

Anti-obesity effect of traditional Chinese medicine and natural products

Chen¹,

Lian²,

Lyu³

et al. 2016

Eur J Bio Med Res

View full text Add to dashboard Cite

Abstract:As obesity has become a worldwide health problem over the past three decades, more emphasis are placed on the prevention and treatment of obesity.Research in the nutrition field has recently aroused considerable interest based on the potential of traditional Chinese medicine and natural products to counteract obesity. This review focuses on the anti-obesity action usingtraditional Chinese medicine and natural products. Some present potent anti-obesity effects because of their ability to reduce blood lipid or blood glucose while others could be used for the treatment of obesity as lipase inhibitor, lipid metabolism regulators, appetite suppressants or fatty acid synthase inhibitor. Further study should be conducted in the mechanisms and active ingredients of traditional Chinese medicine and natural products.

show abstract

“…However, most of these patients will eventually relapse (2). Another challenge of ovarian cancer treatment is chemoresistence, in which DrrAB (drug transporter) may play an important role, which can transport as least seven types of anti-cancer drugs out of cancer cells (35)(36)(37)(38).…”

Section: Treatment Of Ovarian Cancermentioning

confidence: 99%

The possible pathology and pharmaceutical therapy of ovarian cancer

2016

Eur J Bio Med Res

View full text Add to dashboard Cite

Human ovarian cancer is the most lethal gynecological cancer in the western world, causing enomous physical and mental suffering as well as financial hardship to the patients and their families. However, the etiology of the human ovarian cancer is not well understood. To date, multiple etiological factors, including but not limiting to Slit/Robo family, TGF-β family, sex hormones, and angiogenic factors, have been found involved in the pathological process of human ovarian cancer. The involvement of these factors in the human ovarian cancer makes them potential targets for treating human ovarian cancer. Given that human ovarian cancer is highly heterogeneous at both the cellular and molecular levels, the better understanding of actions of these factors and underlying cellular mechanisms in each subtype of human ovarian cancer cells will facilitate the personalized medicine of the lethal disease on the basis of individual characteristics at the cellular and molecular levels.

show abstract

Translational Coupling Controls Expression and Function of the DrrAB Drug Efflux Pump

Cited by 39 publications

References 39 publications

Integrated in vivo and in vitro nascent chain profiling reveals widespread translational pausing

Integrated in vivo and in vitro nascent chain profiling reveals widespread translational pausing

Anti-obesity effect of traditional Chinese medicine and natural products

The possible pathology and pharmaceutical therapy of ovarian cancer

Contact Info

Product

Resources

About