Translational plant biology

Zhang, Dabing

doi:10.1111/jipb.12431

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…According to the correlation between genes in MEblue, we constructed a co-expression network of GhMPK9 and showed several WRKY transcription factors within it (Figure 3B), which were widely considered as the downstream substrates of MAPK. [28] By comparing and clustering the expression profiles of these WRKYs in different RNA-seq samples, we found GhWRKY40a was most closely clustered with GhMPK9 in expression (Figure 3C-E). Finally, we selected GhWRKY40a as a key candidate gene for subsequent analysis.…”

Section: Ghmpk9 and Ghwrky40a Show The Co-expression Profiles And The...mentioning

confidence: 97%

GhMPK9‐GhRAF39_1‐GhWRKY40a Regulates the GhERF1b‐ and GhABF2‐Mediated Pathways to Increase Cotton Disease Resistance

Mi,

Li,

Chen

et al. 2024

Advanced Science

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Mitogen‐activated protein kinase (MAPK) cascade is the center of plant signal transduction system that amplify immune signals into cellular responses by phosphorylating diverse substrates. The MAPK cascade consisting of MAPK kinase kinases (MAPKKKs), MAPK kinases (MAPKKs), and MAPKs is well characterized in plants, in which Raf‐like kinases are generally regarded as MAPKKKs. However, it is rarely reported that Raf‐like MAPKKKs function as middle regulators to link MAPK and its downstream transcription factors in plant immunity. Verticillium wilt, caused by the soil‐borne vascular fungus Verticillium dahliae, is a serious disease in many plants, including cotton. The previous studies showed that GhMPK9 (a MAPK) is involved in the response to Verticillium wilt. Here, the Raf‐like kinase GhRAF39_1 is reported as helper regulates the phosphorylation of WRKY transcription factor GhWRKY40a by GhMPK9. The phosphorylated GhWRKY40a can further activate the transcription of GhERF1b to up‐regulate defense‐related genes while inhibit the transcription of GhABF2 to regulate the stomatal opening, thus improving the resistance to Verticillium wilt in cotton. This study reveals a new signaling module of GhMPK9‐GhRAF39_1‐GhWRKY40a to regulate GhERF1b‐ and GhABF2‐mediated defense responses, which triggers plant defense against Verticillium wilt.

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Section: Ghmpk9 and Ghwrky40a Show The Co-expression Profiles And The...mentioning

confidence: 97%

GhMPK9‐GhRAF39_1‐GhWRKY40a Regulates the GhERF1b‐ and GhABF2‐Mediated Pathways to Increase Cotton Disease Resistance

Mi,

Li,

Chen

et al. 2024

Advanced Science

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“…Labeling is a requirement in several countries to identify GMO-derived products when certain thresholds of maximum content are exceeded. 26 Reliable analytical quantification methods, as well as certified reference materials (CRMs), are mandatory to verify the fulfillment and correct application of these regulations. The majority of CRMs derived from commercially available GMOs are certified for their content of specific events as a mass fraction, the international measurement unit adopted in the analysis and expression of the results.…”

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confidence: 99%

Development, Optimization, and Evaluation of a Duplex Droplet Digital PCR Assay To Quantify the T-nos/hmg Copy Number Ratio in Genetically Modified Maize

Dalmira¹,

Urquiza²,

José-Benigno³

et al. 2015

Anal. Chem.

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Certified reference materials (CRMs) are required to guarantee the reliability of analytical measurements. The CRMs available in the field of genetically modified organisms (GMOs) are characterized using real-time polymerase chain reaction (qPCR). This technology has limited application, because of its dependence on a calibrant. The objective of this study was to obtain a method with higher metrological quality, to characterize the CRMs for their contents of T-nos/hmg copy number ratio in maize. A duplex droplet digital PCR (ddPCR) assay was developed and optimized by a central composite design. The developed method achieved an absolute limit of detection (LOD) of 11 cP T-nos, a relative LOD of 0.034%, a limit of quantification (LOQ) of 23 cP (relative LOQ of 0.08%), and a dynamic range of 0.08%–100% T-nos/hmg ratio. The specificity and applicability of the assay were established for the analysis of low T-nos concentrations (0.9%) in several corn varieties. The convenience of DNA digestion to reduce measurement bias in the case of multiple-copy binding was confirmed through an enzymatic restriction assay. Given its overall performance, this method can be used to characterize CRM candidates for their contents of T-nos/hmg ratio.

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The double-edged sword effect of agricultural technological progress on agricultural carbon emissions: empirical evidence from China

Zhu,

Li,

Wang

et al. 2024

Front. Sustain. Food Syst.

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Global climate change has intensified pressure on China's agricultural carbon emissions reduction. This study investigates the impact of agricultural technological progress on carbon emissions using panel data from 30 Chinese provinces (2002–2021). The research employs the DEA-Malmquist index to measure agricultural total factor productivity, the emission factor method to calculate carbon emissions, and two-tier stochastic frontier models for analysis. Results reveal that agricultural technological progress exhibits a significant double-edged effect on carbon emission intensity, with a positive effect increasing intensity by 9.01% and a negative effect reducing it by 12.18%, resulting in a net reduction of 3.17%. Different technological types show varying impacts: mechanical progress increases emission intensity, while resource-saving and biochemical technologies reduce it. Notable regional heterogeneity exists, with Southwest, South, Central, Northeast, and Northwest regions showing negative net effects, while North and East China exhibit positive effects. The findings suggest the need for differentiated regional policies and targeted agricultural emission reduction strategies to achieve China's agricultural “dual carbon” goals.

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Translational plant biology

Cited by 3 publications

References 10 publications

GhMPK9‐GhRAF39_1‐GhWRKY40a Regulates the GhERF1b‐ and GhABF2‐Mediated Pathways to Increase Cotton Disease Resistance

GhMPK9‐GhRAF39_1‐GhWRKY40a Regulates the GhERF1b‐ and GhABF2‐Mediated Pathways to Increase Cotton Disease Resistance

Development, Optimization, and Evaluation of a Duplex Droplet Digital PCR Assay To Quantify the T-nos/hmg Copy Number Ratio in Genetically Modified Maize

The double-edged sword effect of agricultural technological progress on agricultural carbon emissions: empirical evidence from China

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