Translational regulation contributes to the elevated CO₂ response in two Solanum species

Abstract

Understanding the impact of elevated CO 2 (eCO 2 ) in global agriculture is important given climate change projections. Breeding climate-resilient crops depends on genetic variation within naturally varying populations. The effect of genetic variation in response to eCO 2 is poorly understood, especially in crop species. We describe the different ways in which Solanum lycopersicum and its wild relative S. pennellii respond to eCO 2 , from cell anatomy, to the transcriptome, and metabolome. We further validate the importance of translational regulation as a potential mechanism for plants to adaptively respond to rising levels of atmospheric CO 2 .We measured changes in root and shoot biomass, the photosynthetic rate of leaves, and stomatal conductance under ambient and eCO 2 in S. lycopersicum cv M82 (written as S. lycopersicum throughout) and S. pennellii at 7, 10, and 13 days after planting (DAP) (Figure 1a). It should be noted that differences in plant morphology between these species do not represent a developmental delay (Ron et al., 2013;Koenig et al., 2013). Samples were collected at

Underground gibberellin activity: differential gibberellin response in tomato shoots and roots

“…Previously we showed that GID1a and GID1b1 are highly expressed in elongating stems, while GID1b2 exhibits relatively low expression (Illouz-Eliaz et al, 2019). We analyzed available public data of tomato root transcriptome (Koenig et al, 2013;Zouine et al, 2017;Góra-Sochacka et al, 2019;Gray et al, 2020) and found very low expression of GID1a compared to GID1b1 and GID1b2 in all datasets (Fig. 2i presents the data analyzed from Góra-Sochacka et al, 2019).…”

Underground gibberellin activity: differential gibberellin response in tomato shoots and roots

“…Previously we showed that GID1a and GID1b1 are highly expressed in elongating stems, while GID1b2 exhibits relatively low expression (Illouz-Eliaz et al, 2019). We analyzed available public data of tomato root transcriptome (Koenig et al, 2013;Zouine et al, 2017;G ora-Sochacka et al, 2019;Gray et al, 2020) and found very low expression of GID1a compared to GID1b1 and GID1b2 in all datasets (Fig. 2i presents the data analyzed from G ora- Sochacka et al, 2019).…”

New insights into tomato CLE peptide repertoire and perception mechanisms

“…To investigate the gene structure diversity of tomato CLE s, the exon-intron composition was predicted based on sequence homologies (Figure 1A). In addition, we used publicly available RNAseq datasets [32–34, 45, 46], from root, shoot and fruit samples, to support these gene structure predictions. Reads were mapped on the anticipated coding region of 28 CLE genes out of the 37 newly uncovered loci (Figure 1A).…”