2017
DOI: 10.1261/rna.062208.117
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Translational repression of the Drosophila nanos mRNA involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch

Abstract: Translational repression of maternal mRNAs is an essential regulatory mechanism during early embryonic development. Repression of the mRNA, required for the formation of the anterior-posterior body axis, depends on the protein Smaug binding to two Smaug recognition elements (SREs) in the 3' UTR. In a comprehensive mass spectrometric analysis of the SRE-dependent repressor complex, we identified Smaug, Cup, Me31B, Trailer hitch, eIF4E, and PABPC, in agreement with earlier data. As a novel component, the RNA-dep… Show more

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Cited by 64 publications
(97 citation statements)
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“…Particularly, we noticed that two RNA-regulation proteins, Tral and Cup, were present in both interactomes and the most abundant proteins in the embryo interactome (Supplementary Table 1). This suggests the presence of Me31B-Tral-Cup complexes throughout oogenesis and early embryogenesis, consistent with previous studies that Me31B, Tral, and Cup form complexes and play vital roles in RNA translational regulation or decay in both the ovaries and early embryos 15,[23][24][25][26][27][28] .…”
Section: Resultssupporting
confidence: 91%
“…Particularly, we noticed that two RNA-regulation proteins, Tral and Cup, were present in both interactomes and the most abundant proteins in the embryo interactome (Supplementary Table 1). This suggests the presence of Me31B-Tral-Cup complexes throughout oogenesis and early embryogenesis, consistent with previous studies that Me31B, Tral, and Cup form complexes and play vital roles in RNA translational regulation or decay in both the ovaries and early embryos 15,[23][24][25][26][27][28] .…”
Section: Resultssupporting
confidence: 91%
“…Similar results were seen in previous studies of ME31B complexes in embryonic lysates (Götze et al, 2017). We were also unable to detect CG7611 in any of our samples, despite its ortholog being a component of the human CTLH complex.…”
Section: Degradation Of Me31b Tral and Cup Requires The Ctlh E3 Ligasesupporting
confidence: 92%
“…1H,I). Furthermore, reducing the level of Me31B, which indirectly promotes mRNA decay by repressing translation (Götze et al 2017;Wang et al 2017), enhanced the supernumerary neuroblast phenotype in brat hypomorphic brains ( Fig. 1G).…”
Section: Resultsmentioning
confidence: 98%