Translational research of HAp/agarose composite gel (HAp gel) used as a bone regenerative biomechanical material in jawbone defects

Iwai, Shinichi; Shimizu, Hidetaka; Takeshita, Akinori; Akashi, Mitsuru; Yura, Yoshiaki

doi:10.1016/j.ijom.2013.07.048

Cited by 2 publications

(2 citation statements)

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“…It is noteworthy that both the production method of the bone scaffolds and also their composition are innovative since there are no studies in the literature describing biomaterials made of a chitosan-agarose cryogel matrix reinforced with HA nanopowder. Scientific reports published by other authors present biomaterials for potential bone regenerations which were fabricated by applying completely different production methods and using only two of the mentioned components, e.g., scaffolds composed of only chitosan and hydroxyapatite [11,12], agarose and hydroxyapatite [13,14,15] or chitosan and agarose [16].…”

Section: Introductionmentioning

confidence: 99%

Biological Response to Macroporous Chitosan-Agarose Bone Scaffolds Comprising Mg- and Zn-Doped Nano-Hydroxyapatite

Kazimierczak

Kolmas

Przekora

2019

IJMS

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Modification of implantable scaffolds with magnesium and zinc for improvement of bone regeneration is a growing trend in the engineering of biomaterials. The aim of this study was to synthesize nano-hydroxyapatite substituted with magnesium (Mg2+) (HA-Mg) and zinc (Zn2+) (HA-Zn) ions in order to fabricate chitosan-agarose-hydroxyapatite (HA) scaffolds (chit/aga/HA) with improved biocompatibility. Fabricated biomaterials containing Mg2+ or Zn2+ were tested using osteoblasts and mesenchymal stem cells to determine the effect of incorporated metal ions on cell adhesion, spreading, proliferation, and osteogenic differentiation. The study was conducted in direct contact with the scaffolds (cells were seeded onto the biomaterials) and using fluid extracts of the materials. It demonstrated that incorporation of Mg2+ ions into chit/aga/HA structure increased spreading of the osteoblasts, promoted cell proliferation on the scaffold surface, and enhanced osteocalcin production by mesenchymal stem cells. Although biomaterial containing Zn2+ did not improve cell proliferation, it did enhance type I collagen production by mesenchymal stem cells and extracellular matrix mineralization as compared to cells cultured in a polystyrene well. Nevertheless, scaffolds made of pure HA gave better results than material with Zn2+. Results of the experiments clearly showed that modification of the chit/aga/HA scaffold with Zn2+ did not have any positive impact on cell behavior, whereas, incorporation of Mg2+ ions into its structure may significantly improve biocompatibility of the resultant material, increasing its potential in biomedical applications.

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Section: Introductionmentioning

confidence: 99%

Biological Response to Macroporous Chitosan-Agarose Bone Scaffolds Comprising Mg- and Zn-Doped Nano-Hydroxyapatite

Kazimierczak

Kolmas

Przekora

2019

IJMS

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“…However, there are some papers describing materials for regenerative medicine applications comprising chitosan and agarose, e.g. chitosan/agarose hydrogel wound dressings or cartilage scaffolds [9][10][11], chitosan/HA bone scaffolds [12][13][14] or agarose/HA biomaterials [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Novel synthesis method combining a foaming agent with freeze-drying to obtain hybrid highly macroporous bone scaffolds

Kazimierczak

Benko

Pałka

et al. 2020

Journal of Materials Science & Technology

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Translational research of HAp/agarose composite gel (HAp gel) used as a bone regenerative biomechanical material in jawbone defects

Cited by 2 publications

References 0 publications

Biological Response to Macroporous Chitosan-Agarose Bone Scaffolds Comprising Mg- and Zn-Doped Nano-Hydroxyapatite

Biological Response to Macroporous Chitosan-Agarose Bone Scaffolds Comprising Mg- and Zn-Doped Nano-Hydroxyapatite

Novel synthesis method combining a foaming agent with freeze-drying to obtain hybrid highly macroporous bone scaffolds

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