Translocation (1;16) identified by chromosome painting, and PRimed IN Situ-labeling (PRINS)

Hindkjær, Johnny; Hammoudah, Sahar A.F.M.; Hansen, Knud Bendix; Jensen, Peter D. Ørberg; Koch, Jørn; Pedersen, Bent

doi:10.1016/0165-4608(94)00102-h

Cited by 24 publications

(9 citation statements)

References 20 publications

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“…13 In hematopoietic malignancies, the der(16)t(1;16) has been frequently observed in region 12q13 is also known to contain several genes involved in tumorigenesis such as CHOP, ATF-1, ERBB3, MDM2 and multiple myeloma, 26 but only 12 cases including the present case have been reported in AML or MDS. 19,20 As a result of CDK4. [30][31][32][33] It would be of interest to elucidate whether these genes or unknown putative oncogenes might be implicated with t(1;16), partial trisomy 1q and partial monosomy 16q are usually detected.…”

Section: Molecular Analysis For T(16;21)(p11;q22) Translocationmentioning

confidence: 99%

Establishment of a novel human acute myeloblastic leukemia cell line (YNH-1) with t(16;21), t(1;16) and 12q13 translocations

et al. 1997

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The t(16;21)(p11;q22) translocation is a non-random chromothe fused FUS/ERG protein is shown to function as a transcripsomal aberration observed in several types of human acute tional regulator. 8,11 myeloblastic leukemia (AML), whereas the der(16)t(1;16) and On the other hand, the der (16) A 46-year-old male engineer was admitted to our hospital because of fever in June 1994. Peripheral blood examination Introduction showed white blood cells 120 × 10 9 /l with 91% myeloblasts, hemoglobin 8.3 g/dl and platelets 104 × 10 9 /l. Bone marrow Chromosomal translocations in specific subtypes of leukemia examination revealed 91% myeloblasts that were positive for are recognized to play an important role in the process of myeloperoxidase (MPO) but negative for ␣-naphthyl butylate leukemogenesis by synthesis of chimeric proteins. 1,2 The esterase (␣-NBE) and chloroacetate esterase (CAE) stainings. t(16;21)(p11;q22) translocation is one of the non-random There was no abnormal feature in the eosinophils or monochromosomal aberrations observed in human acute myelocytes. In surface marker analysis the blasts from bone marrow blastic leukemia (AML), blastic crisis of chronic myelogenous were positive for CD13 (79.5%), CD33 (93.3%) and CD34 leukemia (CML) and myelodysplastic syndrome (MDS) that (64.5%). Chromosome analysis of bone marrow cells showed progressed to AML. 3-10 AML with t(16;21) has been classified 46,XY, der(16)t(16;21)(p11;q22)t(1;16)(q12;q13), der (21) An induction therapy was started with enocitabine, daunoruberythrophagocytosis. 3,4 Clinical prognosis of AML with icin, 6-mercaptopurine and prednisolone and complete t(16;21) seems to be poor. Recently, molecular characterizremission (CR) was achieved with normal karyotype. Despite ation of this translocation has revealed that the FUS gene (also two courses of consolidation therapy, the patient relapsed called TLS) encoding an RNA-binding protein on chromosome after 3 months from remission. The second CR was obtained 16 is fused with the ERG gene, a member of the ets gene by low-dose etoposide and aclarubicin treatment in March superfamily, on chromosome 21 to produce the FUS/ERG 1995. However, the second relapse occurred after 1 month chimeric transcript. [7][8][9][10][11] As a result, the RNA-binding domain with blastoma in the colon. The patient died of progressive of FUS is replaced with the DNA-binding domain of ERG and disease and pneumonia in May 1995. CytokinesCorrespondence: H Hamaguchi, Department of Hematology, Musashino Red Cross Hospital, 1-26-1 Kyonan-cho, Musashino-shi, TokyoAll cytokines used in this study, ie recombinant human gra-180, Japan

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Section: Molecular Analysis For T(16;21)(p11;q22) Translocationmentioning

confidence: 99%

Establishment of a novel human acute myeloblastic leukemia cell line (YNH-1) with t(16;21), t(1;16) and 12q13 translocations

et al. 1997

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“…In 19 cases, the der( 16) was associated with the t( 11; 22)(q24;q12) (Lukeis et al, unpublished observations;Mitelman, 1994;Fisher et al, 1995;Hindkjaer et al, 1995;Soukup et al, unpublished observations), the only exceptions being one case with a t(l1;22; 14)(q24;q12;qll) (Turc-Care1 et al, 1988), one case with a t(7;22)(p22;q12) (Squire et al, 1993), and one case with a t(21;22) (Stark et al, 1996). T h e result of the unbalanced t(1;16) was the generation of one or two der(l6) chromosomes, leading to either partial trisomy l q (in 17 cases), partial tetrasomy l q (in five cases), and partial monosomy 16q in all cases.…”

Section: Introductionmentioning

confidence: 93%

“…Fluorescence in situ hybridization (FISH) on metaphase chromosomes with paracentromeric DNA probes has only been performed in breast cancer (Kokalj-Vokac et al, 1993a), with painting probes in multiple myeloma (Mugneret et al, 1995), and with primed in situ labeling (PRINS) in one E T and one acute nonlymphoblastic leukemia (ANLL; Hindkjaer et al, 1995). FISH with satellite and alphoid DNA probes for chromosomes 1 and 16 was used previously for studying the influence of hypomethylation on the chromatin structure in azacytidinc (ACR)-treated lymphocytes and lymphocytes of immunodeficiency, centromeric instability, and facial abnormalities (ICF) syndrome patients (Almeida et al, 1993;Kokalj-Vokac et al, 199313;Miniou et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Demonstration of the translocation der(16)t(1;16)(q12;q11.2) in interphase nuclei of ewing tumors

Hattinger

Rumpler

Ambros

et al. 1996

Genes Chromosom. Cancer

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“…1 B) and preimplantation embryos (Pellestor et al ., 1996aPetit et al, 2000). The use of PRINS has also been reported for analysis of structural aberrations such as translocations, marker chromosomes and ring chromosomes (Brandt et al, 1993;Hindkjaer et al, 1995;Velagaleti et al, 1997). Since the PRINS reaction with Alu primers results in high quality R-like banding on human chromosomes, the procedure has been adapted for the cytogenetic screening of somatic hybrid cell lines and identifi cation of euchromatin in aberrant short arms of acrocentric chromosomes and small ring chromosomes (Callen et al, 1997).…”

Section: Applications and Perspectivesmentioning

confidence: 99%

Multicolor PRINS and multicolor PNA

Pellestor

Paulasova²,

Andréo³

et al. 2006

Cytogenet Genome Res

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Both PRimed IN Situ (PRINS) and Peptide Nucleic Acid (PNA) technologies have emerged as research techniques, but they have quickly evolved to applications in biological diagnosis assays. The two procedures now constitute efficient alternatives to the conventional fluorescence in situ hybridization (FISH) procedure for in situ chromosome identification and aneuploidy detection. They present several advantages (specificity, speed, discriminating ability) that make them very attractive for a number of cytogenetic purposes. Multicolor PRINS and PNA protocols have been described for the specific identification of human chromosomes. Various applications have already been developed in human genetics and new adaptations are ongoing.

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Translocation (1;16) identified by chromosome painting, and PRimed IN Situ-labeling (PRINS)

Cited by 24 publications

References 20 publications

Establishment of a novel human acute myeloblastic leukemia cell line (YNH-1) with t(16;21), t(1;16) and 12q13 translocations

Establishment of a novel human acute myeloblastic leukemia cell line (YNH-1) with t(16;21), t(1;16) and 12q13 translocations

Demonstration of the translocation der(16)t(1;16)(q12;q11.2) in interphase nuclei of ewing tumors

Multicolor PRINS and multicolor PNA

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