Translocation of antibiotic resistance markers of a plasmid-free Streptococcus pyogenes (group A) strain into different streptococcal hemolysin plasmids

Bouguénec, Chantal Le; Horaud, T; Bieth, Gilda; Colimon, R.; Dauguet, C.

doi:10.1007/bf00425548

Cited by 37 publications

(38 citation statements)

References 37 publications

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“…However, since the 1970s multiple antibiotic resistance has begun to appear in this species and a novel class of mobile elements conveniently termed "conjugative transposons" has been shown to be the chief carriers of the multiple antibiotic resistance (5,9,10,14,17,24). The chromosomally integrated conjugative transposons are able to transfer themselves to other cells by a process requiring cell-to-cell contact (5,10,12,23).…”

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Identification and nucleotide sequence analysis of a transfer-related region in the streptococcal conjugative transposon Tn5252

1994

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To obtain a functional map of TnS252, a 47.5-kb streptococcal conjugative transposon, a series of defined deletion and insertion mutations were introduced within the transposon. Interruptions at several regions were found to affect the conjugal transposition functions of the element in filter-mating experiments. The nucleotide sequence of the left terminus of Tn5252 showed two open reading frames, ORFR and ORF2, adjoining the at site. The organization of this region and the structure of the predicted integrase encoded by ORFi were found to be similar to those of other site-specific recombination systems.Even though Streptococcus pneumoniae can receive and stably maintain a variety of plasmids from other streptococci, endogenous plasmids are rare in this species. However, since the 1970s multiple antibiotic resistance has begun to appear in this species and a novel class of mobile elements conveniently termed "conjugative transposons" has been shown to be the chief carriers of the multiple antibiotic resistance (5,9,10,14,17,24). The chromosomally integrated conjugative transposons are able to transfer themselves to other cells by a process requiring cell-to-cell contact (5,10,12,23). Two classes of streptococcal conjugative transposons have been recognized (2, 16). Even though both types are capable of conjugal transposition, no structural similarity has been observed (2, 15). Also, the class of elements, typified by the well-characterized Tn916 and Tn1545, integrate at several sites in the recipient's genome (2,7,8), whereas the larger conjugative transposons such as Tn5252 and Tn3701 preferred to integrate at a unique spot (2, 28). We have been studying the biology of Tn5253, a 65.5-kb conjugative transposon carrying resistance to tetracycline and chloramphenicol (2,(27)(28)(29). In a previous article (2), we showed that TnS253 is a composite element of two conjugative transposons (Fig. 1). The smaller element, now termed TnS251 (18 kb), was observed to be structurally and functionally similar to the Tn916 class of elements (2) and did not show homology to any other region in the parental element, TnS253 (2). The sequences beyond TnS251 within TnS253, now called Tn5252, were themselves capable of conjugative transposition (2). While considerable information regarding the Tn916 (TnS251) class of elements has been obtained (6), very little is known of the Tn5252 class of elements (6). To understand the molecular details of this novel class of elements, we had focused our efforts on studying TnS252. In this article, we report our studies relating to the genetic organization and nucleotide sequence of the left end of this transposon.Strategy for creating insertion and deletion mutations within Tn5252. The availability of Escherichia coli recombinant plasmids carrying DNA fragments derived from TnS252 facilitated generation of deletion and insertion mutations within the passenger segments. The basic strategy employed in this 744-6790. study, illustrated in Fig. 2, was as follows. DNA restriction fragments rangi...

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confidence: 99%

Identification and nucleotide sequence analysis of a transfer-related region in the streptococcal conjugative transposon Tn5252

1994

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“…Isolation of plasmid DNA Plasmid DNA was isolated by a modification of the method of Le Bouguenec et al (1984). Cultures were grown for 18h at 37 'C in 30 ml BHI broth containing 1 % glycine.…”

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confidence: 99%

“…The resistance may be plasmid mediated (Clewell & Franke, 1974;Behnke et al 1979) and has been shown to be transferable by transduction (Hyder & Streitfield, 1978) and conjugation (Le Bouguenec et al 1984), although in the latter case plasmid DNA could not always be demonstrated.…”

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confidence: 99%

A community outbreak of group A beta haemolytic streptococci with transferable resistance to erythromycin

et al. 1989

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SUMMARYErythromycin resistance amongst group A streptococci (GAS) in Great Britain is a relatively rare occurrence and outbreaks have been sporadically reported. Over an 8-month period in 1986 ten associated cases occurred in the town of Bridgwater in Somerset. Isolates were group A, type M4 and resistant to erythromycin (MIC 8 mg/l) but sensitive to lincomycin and clindamycin. Erythromycin resistance was transferable from all isolates to a group A recipient strain. No plasmid DNA could be detected in the original isolates or transconjugants.

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“…A 72-bp region of the target was present on either side following the insertion of Tn5252 and appeared to serve as a signal for its integration and excision. The data suggest that the left copy of the 72-bp segment was a part of the conjugative element, the crossover point of integration was nonrandom within this region, and the mechanism of insertion could resemble that of the site-specific temperate phages.Sudden emergence of multiple-antibiotic resistance in clinical streptococci in the 1970s (6,8,15,16,21) has been chiefly due to a highly promiscuous class of mobile elements termed conjugative transposons (4,12,17,22,29,31). These mediate self-transfer by a DNase-resistant process requiring cell-to-cell contact (8,16,30 BM6001 (8,26), is a 65.5-kb self-transmissible element that encodes resistances to chloramphenicol and tetracycline (29).…”

mentioning

confidence: 99%

“…Sudden emergence of multiple-antibiotic resistance in clinical streptococci in the 1970s (6,8,15,16,21) has been chiefly due to a highly promiscuous class of mobile elements termed conjugative transposons (4,12,17,22,29,31). These mediate self-transfer by a DNase-resistant process requiring cell-to-cell contact (8,16,30 BM6001 (8,26), is a 65.5-kb self-transmissible element that encodes resistances to chloramphenicol and tetracycline (29).…”

mentioning

confidence: 99%

Nucleotide sequence analysis of the termini and chromosomal locus involved in site-specific integration of the streptococcal conjugative transposon Tn5252

Vijayakumar

Ayalew

1993

J Bacteriol

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The 47-kb, broad-host-range, streptococcal conjugative transposon Tn5252 is capable of site-specific integration into the pneumococcal chromosome. We present the nucleotide sequence of the terminal regions of the transposon and its target site in the pneumococcal genome. No inverted repeats were found at the termini of the transposon. A 72-bp region of the target was present on either side following the insertion of Tn5252 and appeared to serve as a signal for its integration and excision. The data suggest that the left copy of the 72-bp segment was a part of the conjugative element, the crossover point of integration was nonrandom within this region, and the mechanism of insertion could resemble that of the site-specific temperate phages.Sudden emergence of multiple-antibiotic resistance in clinical streptococci in the 1970s (6,8,15,16,21) has been chiefly due to a highly promiscuous class of mobile elements termed conjugative transposons (4,12,17,22,29,31). These mediate self-transfer by a DNase-resistant process requiring cell-to-cell contact (8,16,30 BM6001 (8,26), is a 65.5-kb self-transmissible element that encodes resistances to chloramphenicol and tetracycline (29).We had previously cloned the entire element in fragments in Escherichia coli and generated its restriction endonuclease map (34, 35). The transposition behavior of a DNA segment carrying Tetracycline resistance when separated from the context of surrounding DNA led to the identification of TnS253 as a composite structure of two independent conjugative transposons with an 18-kb element, TnS251 (Tcr), inserted in the central region of another, TnS252 (47 kb, Cmr) (2). Similarly, Bouguenec et al., found that Tn3701 in Streptococcus pyogenes was also a composite structure containing a transposon, Tn3703 (Tcr), in its central region (5). TnS251, which is homologous to Tn3703, has been shown to be structurally and functionally very similar to Tn916 and TnlS45 (2, 5). The structure and mechanism of transposition of Tn916 (9,17,18,27,28)

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Translocation of antibiotic resistance markers of a plasmid-free Streptococcus pyogenes (group A) strain into different streptococcal hemolysin plasmids

Cited by 37 publications

References 37 publications

Identification and nucleotide sequence analysis of a transfer-related region in the streptococcal conjugative transposon Tn5252

Identification and nucleotide sequence analysis of a transfer-related region in the streptococcal conjugative transposon Tn5252

A community outbreak of group A beta haemolytic streptococci with transferable resistance to erythromycin

Nucleotide sequence analysis of the termini and chromosomal locus involved in site-specific integration of the streptococcal conjugative transposon Tn5252

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