Translocation of DNA across bacterial membranes.

Dreiseikelmann, Brigitte

doi:10.1128/mmbr.58.3.293-316.1994

Cited by 111 publications

(53 citation statements)

References 211 publications

(244 reference statements)

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“…Synechocystis appears to take up DNA in singlestranded form, similar to what is found for other bacteria [10]. As in Gram-positive bacteria, DNA is taken up without being fragmented [11].…”

Section: Introductionsupporting

confidence: 64%

Transformation of the cyanobacterium Synechocystis sp. PCC 6803 as a tool for genetic mapping: optimization of efficiency

Kufryk

2002

FEMS Microbiology Letters

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The cyanobacterium Synechocystis sp. PCC 6803 is transformable at high efficiency and integrates DNA by homologous double recombination. However, several genetic mapping procedures depend on the ability to generate transformants even with very small amounts of added DNA. This study is aimed at optimizing the transformation efficiency at limiting concentrations of exogenous DNA. The transformation efficiency showed little sensitivity to experimental conditions. Transformation with circular plasmid DNA was found to be no more than 30% more efficient than with linearized plasmid DNA. The efficiency of transformation remained essentially the same in the presence of competing DNA, indicating that the capacity of DNA uptake by the cells is not limiting. The incubation time of cells with DNA before plating (0^8 h) affected the transformation efficiency by up to 3-fold. Only minor changes in the efficiency were observed as a function of the presence of a membrane filter on the plate or the presence of TAE or TBE gel buffer residues in the transformation mixture. However, transformability of the host strain of Synechocystis sp. PCC 6803 was increased by two orders of magnitude if the sll1354 gene encoding the exonuclease RecJ was deleted. Therefore, the transformation efficiency of Synechocystis sp. PCC 6803 with exogenous DNA appears to be determined primarily by intracellular processes such as the efficiency of DNA processing and homologous recombination. ß 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.

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“…Synechocystis appears to take up DNA in singlestranded form, similar to what is found for other bacteria [10]. As in Gram-positive bacteria, DNA is taken up without being fragmented [11].…”

Section: Introductionsupporting

confidence: 64%

Transformation of the cyanobacterium Synechocystis sp. PCC 6803 as a tool for genetic mapping: optimization of efficiency

Kufryk

2002

FEMS Microbiology Letters

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“…Because cell mortality increases with increasing MOI and because UV treatment of particles primarily leads to DNA damage, but does not seem to impact the envelope composition of the particles (Table 4), we assume that 'lysis from without' (Heldal & Bratbak, 1991;Proctor & Fuhrman, 1992;Weinbauer, 2004) is the main process, in which multiple infections lead to the leakage of ions from the cytosol of the recipient cell, resulting in cell death (Dreiseikelmann, 1994;Kivelä et al, 2004). This assumption is partially supported by the fact that autoclaved particles induce low mortality in the recipient cells.…”

Section: Discussionmentioning

confidence: 99%

Evidence for particle-induced horizontal gene transfer and serial transduction between bacteria

Chiura¹,

Kogure²,

Hagemann³

et al. 2011

FEMS Microbiology Ecology

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Incubation of the amino acid-deficient strain Escherichia coli AB1157 with particles harvested from an oligotrophic environment revealed evidence of horizontal gene transfer (HGT) with restoration of all deficiencies in revertant cells with frequencies up to 1.94 × 10(-5). None of the markers were preferentially transferred, indicating that the DNA transfer is performed by generalized transduction. The highest gene transfer frequencies were obtained for single markers, with values up to 1.04 × 10(-2). All revertants were able to produce particles of comparable size, appearing at the beginning of the stationary phase. Examination of the revertants using electron microscopy showed bud-like structures with electron-dense bodies. The particles that display the structural features of membrane vesicles were again infectious to E. coli AB1157, producing new infectious particles able to transduce genetic information, a phenomenon termed serial transduction. Thus, the <0.2-μm particle fraction from seawater contains a particle size fraction with high potential for gene transfer. Biased sinusoidal field gel electrophoresis indicated a DNA content for the particles of 370 kbp, which was higher than that of known membrane vesicles. These findings provide evidence of a new method of HGT, in which mobilizable DNA is trafficked from donor to recipient cells via particles.

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“…T4S systems can be subdivided into three main groups based upon their function: (i) conjugation systems; (ii) DNA release or uptake systems; and (iii) effector translocation systems (Waksman and Fronzes, 2010). Conjugation systems mediate the transfer of DNA to recipient cells in a contact-dependent manner (Dreiseikelmann, 1994). These systems promote genome plasticity in bacteria and thus mediate rapid adaptive responses to changes in environment.…”

Section: Introductionmentioning

confidence: 99%

Structure of a bacterial type IV secretion core complex at subnanometre resolution

Rivera-Calzada

Fronzes

Savva

et al. 2013

EMBO J

105

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Type IV secretion (T4S) systems are able to transport DNAs and/or proteins through the membranes of bacteria. They form large multiprotein complexes consisting of 12 proteins termed VirB1-11 and VirD4. VirB7, 9 and 10 assemble into a 1.07 MegaDalton membrane-spanning core complex (CC), around which all other components assemble. This complex is made of two parts, the O-layer inserted in the outer membrane and the I-layer inserted in the inner membrane. While the structure of the O-layer has been solved by X-ray crystallography, there is no detailed structural information on the I-layer. Using high-resolution cryo-electron microscopy and molecular modelling combined with biochemical approaches, we determined the I-layer structure and located its various components in the electron density. Our results provide new structural insights on the CC, from which the essential features of T4S system mechanisms can be derived.

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Translocation of DNA across bacterial membranes.

Cited by 111 publications

References 211 publications

Transformation of the cyanobacterium Synechocystis sp. PCC 6803 as a tool for genetic mapping: optimization of efficiency

Transformation of the cyanobacterium Synechocystis sp. PCC 6803 as a tool for genetic mapping: optimization of efficiency

Evidence for particle-induced horizontal gene transfer and serial transduction between bacteria

Structure of a bacterial type IV secretion core complex at subnanometre resolution

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