Translocation of isotopically distinct macroalgae: A route to low-cost biomonitoring?

Gröcke, Darren R.; Racionero-Gómez, B.; Marschalek, James; Greenwell, H. Christopher

doi:10.1016/j.chemosphere.2017.06.082

Cited by 10 publications

(21 citation statements)

References 45 publications

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“…Alwyn and Rees 14 found that K m (the concentration of a nutrient that gives half of the maximum rate of uptake) and V max (the maximum rate of uptake achieved at saturating conditions of the nutrient) are greater for ammonium uptake than for nitrate uptake for a range of macroalgal species. However, Gröcke et al 7 concluded that nitrate was taken up at a higher rate than ammonium by Fucus vesiculosus. This finding may be explained by the fact that the initial δ 15 N value of the macroalgae was closer to that of the nitrate solution than that of the ammonium solution.…”

Section: Macroalgal Physiologymentioning

confidence: 99%

“…Light intensity can also produce a small amount of fractionation, with higher light intensity producing more elevated δ 15 N values 15 . This is important to consider when designing translocation experiments, as the depth of the submerged macroalgae will affect the amount of light that can reach them 7 . Gröcke et al 7 found that F. vesiculosus tips submerged at a depth of 1 m below the surface did not isotopically shift as much as tips submerged at 0.2 m from the surface.…”

Section: Macroalgal Physiologymentioning

confidence: 99%

“…This is important to consider when designing translocation experiments, as the depth of the submerged macroalgae will affect the amount of light that can reach them 7 . Gröcke et al 7 found that F. vesiculosus tips submerged at a depth of 1 m below the surface did not isotopically shift as much as tips submerged at 0.2 m from the surface. This could be caused by isotopic fractionation resulting from different environmental conditions between the two depths, or depth stratification of nitrogen sources leading to varying δ 15 N values 7 .…”

Section: Macroalgal Physiologymentioning

confidence: 99%

“…Gröcke et al 7 found that F. vesiculosus tips submerged at a depth of 1 m below the surface did not isotopically shift as much as tips submerged at 0.2 m from the surface. This could be caused by isotopic fractionation resulting from different environmental conditions between the two depths, or depth stratification of nitrogen sources leading to varying δ 15 N values 7 . It is unknown whether temperature‐dependent fractionation affects δ 15 N values in macroalgae, and thus further investigation is needed into this and other environmental factors 33 …”

Section: Macroalgal Physiologymentioning

confidence: 99%

“…Despite these issues, δ 15 N values in macroalgae are generally believed to be representative of biologically available nitrogen in estuaries 15 . Native macroalgae can experience different tidal regimes that could influence the uptake of nitrogen; 17 therefore, translocating macroalgae is considered the optimal method to determine the average δ 15 N values of sources in an area 7 …”

Section: Introductionmentioning

confidence: 99%

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Isotopically labelled macroalgae: A new method for determining sources of excess nitrogen pollution

Bailes

Gröcke

2020

Rapid Comm Mass Spectrometry

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Rationale Stable nitrogen isotope ratios (δ15N) can be used to discern sources of excess nitrogen pollution in water. The δ15N values of nitrate in water often do not reflect the true δ15N source value owing to high temporal variation, and there are high analytical costs associated with obtaining δ15N values from water nitrate. To find alternative solutions, we isotopically labelled macroalgae (i.e. seaweed) beyond natural variation as a new method for determining sources of excess nitrogen pollution in seawater. Methods Fucus vesiculosus (bladder wrack) non‐fertile tips were collected from Easington Colliery, County Durham, UK, and cultured in two isotopically enriched solutions containing ammonium sulphate with δ15N values of 170 ± 5‰ and –60 ± 3‰ for a period of 19 days. The macroalgae were cultured in separate opened glass jars in an incubator with set temperature (11°C) and light (125 μmol photons m−2 s−2 on a light/dark rhythm of 16 h/8 h). The oven‐dried tips were analysed for δ15N over the 19‐day experiment. Results The macroalgal tips incorporated the isotopically enriched solutions rapidly, reaching 50% of the isotopically enriched seawater after ca 11 days for the 15N‐enriched solution and ca 15 days for the 14N‐enriched solution. δ15N values were incorporated more into the torn base of the macroalgal tips than into the middle and apex regions. Conclusions F. vesiculosus rapidly incorporates the isotopic ratio of the artificial seawater solution to which it is translocated. The laboratory‐developed isotopically labelled macroalgae can be manufactured to generate ‘unnatural’ δ15N values for translocation into coastal environments. This approach can provide an efficient, low‐cost alternative to current analytical methods for determining and monitoring nitrogen pollution.

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Section: Macroalgal Physiologymentioning

confidence: 99%

Section: Macroalgal Physiologymentioning

confidence: 99%

Section: Macroalgal Physiologymentioning

confidence: 99%

Section: Macroalgal Physiologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Isotopically labelled macroalgae: A new method for determining sources of excess nitrogen pollution

Bailes

Gröcke

2020

Rapid Comm Mass Spectrometry

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Effects of marine biofertilisation on Celtic bean carbon, nitrogen and sulphur isotopes: Implications for reconstructing past diet and farming practices

Gröcke

Treasure

Lester

et al. 2021

Rapid Comm Mass Spectrometry

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Rationale The application of fertilisers to crops can be monitored and assessed using stable isotope ratios. However, the application of marine biofertilisers (e.g., fish, macroalgae/seaweed) on crop stable isotope ratios has been rarely studied, despite widespread archaeological and historical evidence for the use of marine resources as a soil amendment. Methods A heritage variety of Celtic bean, similar in size and shape to archaeobotanical macrofossils of Vicia faba L., was grown in three 1 × 0.5 m outdoor plots under three soil conditions: natural soil (control); natural soil mixed with macroalgae (seaweed); and 15 cm of natural soil placed on a layer of fish carcasses (Atlantic cod). These experiments were performed over two growing seasons in the same plots. At the end of each growing season, the plants were sampled, measured and analysed for carbon, nitrogen and sulphur stable isotope ratios (δ 13 C, δ 15 N, δ 34 S). Results The bean plants freely uptake the newly bioavailable nutrients (nitrogen and sulphur) and incorporate a marine isotopic ratio into all tissues. The bean δ 15 N values ranged between 0.8‰ and 1.0‰ in the control experiment compared with 2‰ to 3‰ in the macroalgae crop and 8‰ to 17‰ in the cod fish experiment. Their δ 34 S values ranged between 5‰ and 7‰ in the control compared with 15‰ to 16‰ in the macroalgae crop and 9‰ to 12‰ in the cod fish crop. The beans became more 13 C‐depleted (δ 13 C values: 1–1.5‰ lower) due to crop management practices. Conclusions Humans and animals consuming plants grown with marine biofertilisers will incorporate a marine signature. Isotopic enrichment in nitrogen and sulphur using marine resources has significant implications when reconstructing diets and farming practices in archaeological populations.

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Biomonitoring coastal environments with transplanted macroalgae: A methodological review

García-Seoane

Aboal

Boquete

et al. 2018

Marine Pollution Bulletin

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Translocation of isotopically distinct macroalgae: A route to low-cost biomonitoring?

Cited by 10 publications

References 45 publications

Isotopically labelled macroalgae: A new method for determining sources of excess nitrogen pollution

Isotopically labelled macroalgae: A new method for determining sources of excess nitrogen pollution

Effects of marine biofertilisation on Celtic bean carbon, nitrogen and sulphur isotopes: Implications for reconstructing past diet and farming practices

Biomonitoring coastal environments with transplanted macroalgae: A methodological review

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