Translocation yields in peripheral blood lymphocytes from control populations

Ca, Whitehouse; Aa, Edwards; Ej, Tawn; Stephan, G.; Oestreicher, Ursula; Je, Moquet; Dc, Lloyd; Roy, L.; Voisin, P.; Lindholm, Carita; Barquinero, Joan Francesc; Barrios, Leonardo; Mr, Caballín; Darroudi, F.; Fomina, J.

doi:10.1080/09553000500103082

Cited by 56 publications

(39 citation statements)

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“…De nombreuses publications attestent que le taux de translocations augmente significativement avec l'âge (Darroudi, 2000 ;Edwards et al, 2005 ;Pressl et al, 1999 ;Ramsey et al, 1995 ;Sigurdson et al, 2008 ;Sorokine-Durm et al, 2000a ;Whitehouse et al, 2005). Ces études peuvent être regroupées en deux groupes : celles regroupant les données issues de plusieurs laboratoires pour avoir plus de poids statistique (Sigurdson et al, 2008 ;Sorokine-Durm et al, 2000b ;Whitehouse et al, 2005), et celles qui présentent les données d'un seul laboratoire (Darroudi et Natarajan, 2000 ;Pressl et al, 1999 ;Ramsey et al, 1995) (Tab.…”

Section: Effet De L'âgeunclassified

“…Ces études peuvent être regroupées en deux groupes : celles regroupant les données issues de plusieurs laboratoires pour avoir plus de poids statistique (Sigurdson et al, 2008 ;Sorokine-Durm et al, 2000b ;Whitehouse et al, 2005), et celles qui présentent les données d'un seul laboratoire (Darroudi et Natarajan, 2000 ;Pressl et al, 1999 ;Ramsey et al, 1995) (Tab. II).…”

Section: Effet De L'âgeunclassified

“…Cela confirme l'importance du choix de la population la plus jeune dans la comparaison avec une population la plus âgée. En effet, l'augmentation du taux de translocation est souvent significative quand le groupe le plus jeune est composé de nouveaux-nés (Ramsey et al, 1995 ;Sigurdson et al, 2008 ;Whitehouse et al, 2005). L'étude européenne de Whitehouse et al (2005) montre également une augmentation du taux de translocations avec l'âge pour tous les laboratoires avec une augmentation plus significative après 60 ans .…”

Section: Impact Des Facteurs Individuels Et Environnementauxunclassified

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Impact des facteurs individuels et environnementaux sur le taux d’aberrations chromosomiques de type translocations Partie 1 : âge, sexe, tabac, alcool

et al. 2010

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The assessment of exposure to ionizing radiation, carried out long time after exposure, is currently performed by scoring of translocations, a specific type of chromosomal aberrations. The translocations rate observed in peripheral blood lymphocytes of exposed subjects is compared to that observed in a control population. However, the translocation specificity towards radiation exposure is not clearly identified. To avoid any hasty conclusion, it is necessary to identify all the factors likely to induce translocation. To our knowledge, no study has thus far examined the effects of all these different factors on translocation rates. A review of the literature thus allowed us to assess the impact of host factors and lifestyle on the production of translocations. This study confirms that age appears to be the factor 1 IRSN, Laboratoire de Dosimétrie Biologique, BP 17, 92262 Fontenay-aux-Roses Cedex, France.Article publié par EDP Sciences E. GRÉGOIRE et al. 154 RADIOPROTECTION -VOL. 45 -N° 2 (2010) having the greatest impact on the rate of translocations, especially over 60 years. To date, the factor "age" is already considered in estimating the impact of radiation on the rate of translocation for all age groups. However, the study also shows that this rate varies significantly when the patient is exposed simultaneously and significantly towards many lifestyle agents. A precise threshold translocation rate should thus be established as a function of known behavioral exposures, below which it is impossible to conclude that radiological exposure has occurred. The effects of chemicals on the translocation rate after occupational exposure will be the subject of a second part.

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Section: Effet De L'âgeunclassified

Section: Impact Des Facteurs Individuels Et Environnementauxunclassified

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Impact des facteurs individuels et environnementaux sur le taux d’aberrations chromosomiques de type translocations Partie 1 : âge, sexe, tabac, alcool

et al. 2010

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“…The calculation of the biological radiation doses on the frequency of reciprocal translocations using 3-4 chromosomes (representing approximately 20% of the genome) showed that FISH technique can be used for retrospective group dosimetry for the doses above 25 cGy (one should score 1000 cells, genome equivalent) and for the individual dosimetry 2000 cells genome equivalent is recommended. For situation of exposure in dose range ~ 30 -200 cGy, 1000, and 500 cells genome equivalent, respectively should be sufficient, taking into account the factor of aging [18,20,21]. In order to reduce number of cells for analysis and to detect all structural as well as numerical aberrations simultaneously in one preparations a multi-colour FISH technique is developed and applied for whole genome analysis of human chromosomes using 23/24 different colours for 23/24 different human chromosomes [26].…”

Section: Post Chernobyl Experiences (In Human Studies)mentioning

confidence: 99%

“…Among these assays the control level (unexposed samples) of chromosomal alterations is in order of: micronuclei (3-35) > translocations (0-15) > dicentrics (1-4) > PCC (0-2) per 1000 cells analyzed [6]. For both MN and translocation assays influence of age on increasing level of chromosomal alterations was evident [6,20,21]. Moreover, smoking status suggests an acceleration of translocation ≥ age of 60 years old [21].…”

Section: Frequency Of Spontaneously Occurring Chromosomal Alterationsmentioning

confidence: 99%

Cytogenetic Biological Dosimetry Past, Present and Future Perspectives

Darroudi

2012

Sixth International Conference on Environmental Mutagens in Human Populations

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Abstract. Human risk assessments at the low doses, and low dose rates and high doses following acute exposure to ionizing radiation are of prime importance in radiation protection. These issues are of continuing importance in respect of social/economic policy relating to the industrial and medical uses of ionizing radiation, and for risk assessment among people occupationally are being exposed to low and/or high LET radiation, such as astronauts, pilots, stewardess and nuclear power plant workers, as well as victims of radiation accidents. Consequently, several biological assays were developed and attempts were made to investigate formation of radiation induced chromosome aberrations and induction of genomic instability in human lymphocytes and fibroblasts. Fluorescence in situ hybridization (FISH) technique using chromosome, chromosome-arm, chromosome region, centromere and telomere specific DNA libraries has improved the resolution of detecting all classes of radiation induced chromosomal inter-and intra-changes. Consequently, has increased significantly the accuracy and detection limit of biological dosimetry. Newly obtained data indicate that (a) Premature chromosome condensation assay is a unique method to be used for immediate dose assessment at low (5cGy) as well as high doses (≥3 Gy) and can accurately discriminate between whole-and partial-body exposure in case of mass casualties and accidental over-exposure to high doses of ionizing radiation (b) FISH-based translocation assay has the potential to assess acute as well as chronic exposure in cases of accidental as well as occupational exposure to ionizing radiation, either immediately following exposure, or retrospectively by defining accumulative effects to red bone marrows. (c) there are distinct finger-prints (such as insertions and complex translocations) for high LET radiation in comparison to low LET radiation. The importance of these findings and future perspectives for biological dosimetry are discussed.

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Biweekly paclitaxel, cisplatin, tegafur, and leucovorin as neoadjuvant chemotherapy for unresectable squamous cell carcinoma of the head and neck

Wang

Liao

Chang

et al. 2004

Cancer

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BACKGROUNDThe goal of the current study was to evaluate the efficacy and toxicity of paclitaxel, cisplatin (P), tegafur (T), and leucovorin (L) as a neoadjuvant chemotherapy (CT) for patients with advanced, unresectable squamous cell carcinoma of the head and neck.METHODSFrom November 1999 to January 2001, 21 consecutive patients (Stage IV, 100%; T4, 86%; and N3, 41%) were treated with paclitaxel‐PTL (Day 1: paclitaxel, 120 mg/m2 intravenous infusion for 3 hours; Day 1: P, 50 mg/m2; T, 800 mg; and L, 60 mg orally daily over a 14‐day cycle). Evaluation after three cycles led to CT termination if primary tumor responses were less than partial responses. Otherwise, paclitaxel‐PTL was continued for up to six cycles before commencement of locoregional therapy.RESULTSCT responses were analyzed on an intent‐to‐treat basis. Response rates (RR) for the primary tumors were 81% (17 of 21), with 28.6% (6 of 21) showing a complete response (CR). RR and CR rates for the neck lymph nodes were 85.3% (15 of 18) and 22% (4 of 18), respectively. The combined RR for primary tumors and neck lymph nodes was 81% (95% confidence interval, 62.9–99.3%) with a CR rate of 19%. Grade 3/4 toxicities according to World Health Organization criteria included leukopenia, 19.0%; emesis, 9.5%; asthenia, 9.5%; mucositis, 4.8%; and neuropathy, 4.8%. Both the overall and disease‐free survival rates were 14.3% (3 of 21), with a median follow‐up of 41 months.CONCLUSIONSThe relatively low toxicities and encouraging response rates demonstrated in the current study suggested that paclitaxel‐PTL merits future trials in the setting of resectable tumors with more favorable characteristics. Cancer 2004. © 2004 American Cancer Society.

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Translocation yields in peripheral blood lymphocytes from control populations

Abstract: For retrospective biological dosimetry purposes a generic age-dependent control level can be assumed. No other lifestyle factors such as smoking appear to have a significant effect on translocation yield.

Cited by 56 publications

References 11 publications

Impact des facteurs individuels et environnementaux sur le taux d’aberrations chromosomiques de type translocations Partie 1 : âge, sexe, tabac, alcool

Impact des facteurs individuels et environnementaux sur le taux d’aberrations chromosomiques de type translocations Partie 1 : âge, sexe, tabac, alcool

Cytogenetic Biological Dosimetry Past, Present and Future Perspectives

Biweekly paclitaxel, cisplatin, tegafur, and leucovorin as neoadjuvant chemotherapy for unresectable squamous cell carcinoma of the head and neck

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