Transmembrane electrochemistry of erythrocytes: Direct electrochemical test for detecting hemolysis in whole blood

Doménech‐Carbó, Antonio; Villamón, Eva; Luna, Irene; Ramos, David; Doménech-Casasús, Clara; Cebrián‐Torrejón, Gerardo

doi:10.1016/j.snb.2015.12.001

Cited by 16 publications

(4 citation statements)

References 54 publications

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“…The applications of electrochemical methods for these purposes are advantageous mostly because of the practical characteristics of electrochemical devices notably fast response, rapid diagnostic, and cost‐effective materials which can be used in situ and often at point of care. Recently, few reports have suggested that red blood cells (RBC) can be electrochemically detected directly on various electrodes . Herein, an analytical method for a fast count of red blood cells is presented, under conditions suitable for a point‐of‐care test.…”

Section: Figurementioning

confidence: 99%

Electrochemical Red Blood Cell Counting: One at a Time

et al. 2016

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We demonstrate that the concentration of a red blood cell solution under physiological conditions can be determined by electrochemical voltammetry. The magnitude of the oxygen reduction currents produced at an edge‐plane pyrolytic graphite electrode was diagnosed analytically at concentrations suitable for a point‐of‐care test device. The currents could be further enhanced when the solution of red blood cells was exposed to hydrogen peroxide. We show that the enhanced signal can be used to detect red blood cells at a single entity level. The method presented relies on the catalytic activity of red blood cells towards hydrogen peroxide and on surface‐induced haemolysis. Each single cell activity is expressed as current spikes decaying within a few seconds back to the background current. The frequency of such current spikes is proportional to the concentration of cells in solution.

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Section: Figurementioning

confidence: 99%

Electrochemical Red Blood Cell Counting: One at a Time

et al. 2016

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“…Electrochemical methods are sensitive, are financially viable and have a variety of instrument styles and components [ 27 ]. These techniques are useful for detecting a diversity of analytes [ 28 , 29 , 30 ]. Potentiometric and amperometric systems can be used sequentially in the monitoring of industrial waste [ 31 ]; they detect, through measurable electrical signals, conformational changes in biomolecules when in contact with a ligand [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Characterization Using Biosensors with the Coagulant Moringa oleifera Seed Lectin (cMoL)

et al. 2023

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Triturated Moringa oleifera seeds have components that adsorb recalcitrant indigo carmine dye. Coagulating proteins known as lectins (carbohydrate-binding proteins) have already been purified from the powder of these seeds, in milligram amounts. The coagulant lectin from M. oleifera seeds (cMoL) was characterized by potentiometry and scanning electron microscopy (SEM) using MOFs, or metal–organic frameworks, of [Cu3(BTC)2(H2O)3]n to immobilize cMoL and construct biosensors. The potentiometric biosensor revealed an increase in the electrochemical potential resulting from the Pt/MOF/cMoL interaction with different concentrations of galactose in the electrolytic medium. The developed aluminum batteries constructed with recycled cans degraded an indigo carmine dye solution; the oxide reduction reactions of the batteries generated Al(OH)3, promoting dye electrocoagulation. Biosensors were used to investigate cMoL interactions with a specific galactose concentration and monitored residual dye. SEM revealed the components of the electrode assembly steps. Cyclic voltammetry showed differentiated redox peaks related to dye residue quantification by cMoL. Electrochemical systems were used to evaluate cMoL interactions with galactose ligands and efficiently degraded dye. Biosensors could be used for lectin characterization and monitoring dye residues in environmental effluents of the textile industry.

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“…Accordingly, the development of analytical methods oriented to their effective implementation into the clinical practice is of interest in particular in regard to routine analysis. With this objective, we have previously reported electrochemical methodologies for screening antimalarial drugs [20] and detecting hemolysis in blood [21], and bladder cancer in urine [22].…”

Section: Introductionmentioning

confidence: 99%

Biomedical application of VIMP: screening of malignant cells in the prostate

Doménech‐Carbó

Doménech-Casasús²,

Pontones

et al. 2020

J Solid State Electrochem

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An electrochemical method for discriminating healthy and malignant tissues in prostate biopsies using the voltammetry of immobilized particles methodology is described. The method involves the sampling with graphite bars 0.5 mm in diameter on paraffin-impregnated cross sections of prostate tissues used in ordinary cytological evaluation after local staining with methylene blue (MB). The subsequent record of the voltammetric response of sample-modified graphite electrodes displays clearly different MB-centered features for healthy and malignant regions due to the different association of the dye to the respective cells.

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Transmembrane electrochemistry of erythrocytes: Direct electrochemical test for detecting hemolysis in whole blood

Cited by 16 publications

References 54 publications

Electrochemical Red Blood Cell Counting: One at a Time

Electrochemical Red Blood Cell Counting: One at a Time

Electrochemical Characterization Using Biosensors with the Coagulant Moringa oleifera Seed Lectin (cMoL)

Biomedical application of VIMP: screening of malignant cells in the prostate

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