Transmembrane potentials in guinea‐pig hepatocytes

Heller, Philip H.; Kloot, William Van der

doi:10.1113/jphysiol.1974.sp010767

Cited by 18 publications

(7 citation statements)

References 73 publications

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“…In some of these cells, biologically important electrical responses can be detected (e.g. action potentials ; Kaneko, 1969) and electrogenic effects (Heller & van der Kloot, 1974;Petersen, 1974). Most recently, Table 4…”

Section: ;mentioning

confidence: 99%

The Mitochondrial Membrane Potential

Tedeschi

1980

Biological Reviews

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Section: ;mentioning

confidence: 99%

The Mitochondrial Membrane Potential

Tedeschi

1980

Biological Reviews

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“…A high K+ and low Na+ concentration within the cells is maintained by a coupled ouabain-sensitive active transport (Elshove & van Rossum, 1963;Haylett & Jenkinson, 1972a, b;Heller & van der Kloot, 1974; , exhibiting an electrogenic component (Claret & Mazet, 1972 a;Graf & Petersen, 1974).…”

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Cell membrane potential and resistance in liver.

Graf¹,

Petersen²

1978

The Journal of Physiology

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1. Isolated segments of mouse liver were placed in a Perspex bath through which physiological saline solutions of varying composition were circulated. Two microelectrodes were inserted in different liver cells under microscopic control allowing measurement of distance between the two micro-electrode tips. Current pulses were injected through one of these electrodes, causing electrotonic potential changes in nearby cells by current spread through intercellular junctions. These electrotonic potential changes were recorded with the second micro-electrode. The spatial decrement of the amplitude of the electrotonzpotential changes and their dependence on extracellular ion concentrations were analysed by three-dimensional cable analysis, modified to account for the geometry of the tissue. 2. During exposure to control solution the mean resting cell membrane potential was -37 mV, the space constant for intracellular current spread (lambda3 = square root of Rm/chrRi) was 390 micron and Ri, a measure which includes the intracellular resistivity and the junctional resistances, was 1.4 komegacm. From these values, and an estimate of tissue cell membrane density (chi) obtained by others, the specific membrane resistance (Rm) was calculated to be 5.1 komegacm2. 3. Replacement of extracellular Na+ by K+ resulted in a large depolarization and a large decrease in the membrane resistance. Replacement of extracellular Na+ by choline resulted in a small transient hyperpolarization and a small increase in the membrane resistance. Replacement of extracellular Cl- by methylsulphate or sulphate or of NaCl by sucrose resulted in a small transient depolarization and a large increase in the membrane resistance. 4. Glucagon (10(-7) M) and adrenaline (10(-5) M) evoked membrane hyperpolarization and reduction of membrane resistance (Rm). 5. The resting membrane ion conductance can be considered to consist of three components, Cl conductance (GCl), GK and GNa. The results suggest that GCl greater than GK greater than GNa. Changes in extracellular ion concentrations specifically alter the permeability properties of the cell membrane. The glucagon action can be explained in part by an increase in GK.

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“…Lassen et al, working with Ehrlich ascites tumor cells (21) or Amphiuma erythrocytes (22), found that following impalement there was a rapid decay of the membrane potential with a half time of about 1 ms. After the decay, the steady-state potential was no longer sensitive to the K § concentration of the medium. They calculate that, given the lesser surface areas of mitochondria, the decay after impalement should be much faster (21 5,6,7,11,12,17,18,37,39,40,41]). Potentials which do not decay significantly with time have been recorded (5,6,7,12,17,(37)(38)(39)(40)(41).…”

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“…They calculate that, given the lesser surface areas of mitochondria, the decay after impalement should be much faster (21 5,6,7,11,12,17,18,37,39,40,41]). Potentials which do not decay significantly with time have been recorded (5,6,7,12,17,(37)(38)(39)(40)(41). Some of these potentials were found to be sensitive to the K § concentration in the medium (5,6,12,39,40).…”

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Membrane potentials and resistances of giant mitochondria. Metabolic dependence and the effects of valinomycin.

Maloff¹,

Scordilis²,

Reynolds³

et al. 1978

The Journal of Cell Biology

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The membrane potentials and resistances of giant mitochondria from mice fed cuprizone have been studied. They were found to correspond approx. 10-20 mV, positive inside, and 2 M~, respectively. These properties were found to be independent of the metabolic state. The microelectrodes were in the inner mitochondrial space since (a) the potentials in the presence of valinomycin depended on the K § concentration of the medium and the magnitude of the K § diffusion potentials was consistent with the presence of a high internal concentration of K § (b) almost identical results were obtained with mitochondria from which the external membrane had been removed and the cristae were evaginated, and (c) punch-through experiments, in which the microelectrodes were advanced until they emerged through the other side of the mitochondria, showed an identical membrane potential both in the presence and in the absence of valinomycin. The potentials were stable under a variety of conditions and showed no sign of decay or membrane leakiness.Detailed evidence that the impaled mitochondria are metabolically viable will be presented in a separate publication. KEY WORDS mitochondrial potentials microelectrodes oxidative phosphorylationThe Mitchell chemiosmotic hypothesis proposes that the electrochemical potential gradient of H § across membranes (the so-called protonmotive force) plays the major role in mitochondrial phosphorylation and the transport of ions. This hypothesis has been described in detail in a series of publications (e.g., references 28-34). The electrochemical potential gradient of H +, which is the result of proton pumps (in the form of the socalled loops of Mitchell), is constituted of the H § concentration gradient and the membrane potential.Tupper and Tedeschi (50, 52-54) were able to measure membrane potentials and membrane resistances directly in Drosophila giant mitochondria by means of microelectrodes driven by a piezoelectric device. The membrane potential was found to range from 10 to 20 mV, positive inside, and the resistance was 1-4 f~cm ~. Metabolism was not found to affect either the potentials or resistances significantly (53). The specific resistances were J. CELL BIOLOGY 9 The Rockefeller University Press 9 0021-9525/78

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Transmembrane potentials in guinea‐pig hepatocytes

Cited by 18 publications

References 73 publications

The Mitochondrial Membrane Potential

The Mitochondrial Membrane Potential

Cell membrane potential and resistance in liver.

Membrane potentials and resistances of giant mitochondria. Metabolic dependence and the effects of valinomycin.

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