Transmissible Citrate‐Utilizing Ability in <i>Escherichia coli</i> Isolated from Pigeons, Pigs and Cattle

Sato, Gihei; Asagi, M; Oka, Chiaki; Ishiguro, Naotaka; Terakado, Nobuaki

doi:10.1111/j.1348-0421.1978.tb00380.x

Cited by 26 publications

(26 citation statements)

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“…The citrate-fermenting ability of these gram-negative bacteria appears to be linked to the presence of genetically unstable determinants such as plasmids (13,14,18,32,33,37,38) (12,36,41), the energetics of citrate uptake are not yet understood. Kempler and McKay (19) demonstrated that the ability to transport citrate was linked to a 7.9-kilobase (kb) plasmid that appears to be present in all citrate-fermenting L. lactis strains analyzed.…”

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confidence: 99%

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Nucleotide sequence and expression in Escherichia coli of the Lactococcus lactis citrate permease gene

David¹,

Rest²,

Driessen³

et al. 1990

J Bacteriol

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The plasmid-encoded citrate determinant of the Lactococcus lactis subsp. lactis var. diacetylactis NCDO176 was cloned and functionally expressed in a Cit-Escherichia coli K-12 strain. From deletion derivative analysis, a 3.4-kilobase region was identified which encodes the ability to transport citrate. Analysis of proteins encoded by the cloned fragment in a T7 expression system revealed a 32,000-dalton protein band, which correlated with the ability of cells to transport citrate. Energy-dependent [1,5-'4Cjcitrate transport was found with membrane vesicles prepared from E. coli cells harboring the citrate permease-expressing plasmid. The gene encoding citrate transport activity, citP, was located on the cloned fragment by introducing a site-specific mutation that abolished citrate transport and resulted in a truncated form of the 32,000-dalton expression product. The nucleotide sequence for a 2.2-kilobase fragment that includes the citP gene contained an open reading frame of 1,325 base pairs coding for a very hydrophobic protein of 442 amino acids, which shows no sequence homology with known citrate carriers.As in members of the family Enterobacteriaceae (25), the ability to utilize citrate is a useful metabolic characteristic for identifying Lactococcus lactis species (6,34). The citrate-fermenting ability of these gram-negative bacteria appears to be linked to the presence of genetically unstable determinants such as plasmids (13,14,18,32,33,37,38) (12,36,41), the energetics of citrate uptake are not yet understood. Kempler and McKay (19) demonstrated that the ability to transport citrate was linked to a 7.9-kilobase (kb) plasmid that appears to be present in all citrate-fermenting L. lactis strains analyzed. A detailed physical map of one of these citrate plasmids, pCT176, has been reported (10).In the bacterial species described until now, the ability to grow on citrate is associated with cation-dependent transport systems. Na+-dependent citrate utilization is found in Enterobacter aerogenes (16,28) and Salmonella typhimurium, which also possess a K+-dependent transport system (1,18,40 Media and growth conditions. E. coli strains were grown in L-broth (24) with vigorous shaking at 37°C. When appropriate, the medium was supplemented with carbenicillin (100 ,ug/ml), kanamycin (20 p,g/ml), or tetracycline (12.5 ,ug/ml) or a combination of these antibiotics.Citrate-positive recombinants of E. coli DH1 were selected after overnight incubation on Simmons citrate agar plates (Difco Laboratories).Cloning of the citP gene. CsCl-ethidium bromide density gradient-purified plasmid DNA from L. lactis NCDO176 was prepared by the method of Maniatis et al. (24) with minor variations as described previously (6) and was digested to completion with EcoRI. The 7.9-kb linearized plasmid band of pCT176 was isolated, inserted into the unique EcoRI site of vector pBR328, and transformed to E. coli MC1061.

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“…The citrate-fermenting ability of these gram-negative bacteria appears to be linked to the presence of genetically unstable determinants such as plasmids (13,14,18,32,33,37,38) or transposons (15). The presence of plasmid-or transposonencoded citrate transport systems enables members of the Enterobacteriaceae to utilize citrate as the sole carbon source.…”

mentioning

confidence: 99%

Nucleotide sequence and expression in Escherichia coli of the Lactococcus lactis citrate permease gene

David¹,

Rest²,

Driessen³

et al. 1990

J Bacteriol

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“…The Cit character and drug resistance markers of R plasmids were co-transmitted by conjugation in many transconjugants tested. But, since transconjugants carrying only the citrate-utilizing determinant were found during transfer experiments except in some of the human isolates (Table 6), it can be concluded that the Cit character, like those for an H2S-positive E. coli strain, is located on a transmissible plasmid (Cit plasmid) which differs from R plasmid, as described by Sato et al (1978) …”

Section: Discussionmentioning

confidence: 99%

The distribution of plasmids determining citrate utilization in citratè-positive variants ofEscherichia colifrom humans, domestic animals, feral birds and environments

Ishiguro

Sato

1979

J. Hyg.

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SUMMARYSixty-seven isolates of citrate-positive variants of Escherichia coli were isolated from human, domestic animal, feral bird and environmental sources. With the exception of citrate utilization, all isolates were identified as typical E. coli by their biochemical reactions. The transmission of the ability to utilize citrate on Simmons' citrate agar was demonstrated in 53 (79.1 %) out of the 67 citratepositive E. coli variants obtained from various sources. Drug resistance determinants and citrate utilizing character were co-transmitted into E. coli K-12 by conjugation among citrate-positive E. coli isolates carrying R plasmids except for that isolated from horses. The other characters (haemolysin or colicin production, raffinose or sucrose fermentation) were not transmitted together with the citrate utilizing character. These facts suggested that the structural gene responsible for citrate utilizing ability in citrate-positive variants of E. coli was located on a conjugative plasmid.

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“…They were obtained by direct cultivation, using MacConkey agar (Eiken) plates, and identified as E. coli by 34 biochemical tests (Ishiguro, Oka & Sato, 1978).…”

Section: E Coli Strains Examinedmentioning

confidence: 99%

Genetical relationship between R plasmids derived fromSalmonellaandEscherichia coliobtained from a pig farm, and its epidemiological significance

Ishiguro

Goto

Sato

1980

J. Hyg.

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SUMMARYA total of 475 Salmonella strains belonging to 5 serovars, isolated from a pig farm which had been heavily contaminated with Salmonella for the past 2 years were tested for antibiotic susceptibility and detection of R plasmids. Thirty-three Escherichia coli isolates from the same farm were also examined in a similar way.Out of 475 strains 348 (73.2 %) were resistant to one or more antibiotics such as tetracycline (Tc), streptomycin (Sm), sulfadimethoxine (Su), chloramphenicol (Cm) and kanamycin (Km), and 247 (85.2 %) out of 290 strains belonging to 3 serovars examined harboured conjugative R plasmids. There was no change in the pattern of drug resistance during this survey nor any variation in the pattern of resistance of R plasmids, whatever the serovar. The antibiogram pattern Tc Sm Su, mainly S. typhimurium, was common among Salmonella strains. Among the transferred resistance patterns, the thermosensitive R plasmids conferring the Tc marker detected in this study were Fi-, and belonged to incompatibility group Hi, whereas the R plasmids conferring Sm Su resistances which coexisted in the same host were Fi+, and compatible with the reference R plasmids tested. The Ioc plasmid conferring Cm resistance alone was isolated from S. anatum and the FII plasmid conferring Sm Km resistances was also isolated from S. typhimurium. In contrast, the 33 E. coli strains examined were resistant to three or five antibiotics and most of the resistance markers were located on conjugative R plasmids. Ioc plasmids conferring Cm resistance alone or FII plasmids conferring Cm or Km markers were common in the E. coli strains. Hi and H2 plasmids conferring multiple resistance markers were also found in them. The genetic properties of R plasmids derived from Salmonella or E. coli strains are compared, and the potential spread of R plasmids between strains of Salmonella and E. coli is discussed.

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Transmissible Citrate‐Utilizing Ability in Escherichia coli Isolated from Pigeons, Pigs and Cattle

Cited by 26 publications

References 4 publications

Nucleotide sequence and expression in Escherichia coli of the Lactococcus lactis citrate permease gene

Nucleotide sequence and expression in Escherichia coli of the Lactococcus lactis citrate permease gene

The distribution of plasmids determining citrate utilization in citratè-positive variants ofEscherichia colifrom humans, domestic animals, feral birds and environments

Genetical relationship between R plasmids derived fromSalmonellaandEscherichia coliobtained from a pig farm, and its epidemiological significance

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