Transmission muography is a non-invasive imaging technique that exploits the penetrating power of atmospheric muons into matter to obtain two-dimensional and three-dimensional density images of the monitored structure. The detectors used are particle trackers. Muography enables the monitoring of large structures and it is also particularly useful in the archaeological field for a mapping of low-density underground anomalies potentially related to unknown or inaccessible tombs or tunnels. The Palazzone necropolis, located south of Perugia (Italy), dating back to Etruscan period, contains about 200 known tombs, some of which, such as the Volumni Hypogeum, can be visited thanks to a touristic route. The eastern area of the necropolis, on the other hand, does not have a touristic path and is partially unknown. The objective of the muographic measurement campaign is to support the re-evaluation of this archaeological area by searching for new anthropic cavities and identifying them three-dimensionally. One of the goals of this study is to obtain a three-dimensional localization of cavities starting from a single muographic measurement by exploiting an image focusing algorithm. For this purpose, an area that contains a known cavity was used as the reference cavity for the test of the three-dimensional reconstruction algorithm.