Transmission electron microscopy of rabbit liver after high-intensity focused ultrasound ablation combined with ultrasound contrast agents

Jiang, Ying; Tian, Xue; Luo, Wen; Zhou, Xiaodong

doi:10.1007/bf02849963

Cited by 5 publications

(3 citation statements)

References 12 publications

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“…Another study, using highintensity focused ultrasound (HIFU) (intensity not specified) for ablating the liver in rabbits, also observed their treated tissues with TEM. In this study, in cells that were not completely disrupted by the exposures, the mitochondria and ER were both found to be distended where the latter appeared as large circular vacuoles (33). On the basis of the results of the present study, where substantially lower intensities were used, it is not unreasonable to assume that these more robust structural changes could have occurred due to the higher-intensity exposures.…”

Section: Discussionmentioning

confidence: 61%

Intramembrane cavitation as a unifying mechanism for ultrasound-induced bioeffects

Krasovitski

Frenkel

Shoham

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

462

375

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The purpose of this study was to develop a unified model capable of explaining the mechanisms of interaction of ultrasound and biological tissue at both the diagnostic nonthermal, noncavitational (<100 mW·cm −2) and therapeutic, potentially cavitational (>100 mW·cm −2 ) spatial peak temporal average intensity levels. The cellular-level model (termed "bilayer sonophore") combines the physics of bubble dynamics with cell biomechanics to determine the dynamic behavior of the two lipid bilayer membrane leaflets. The existence of such a unified model could potentially pave the way to a number of controlled ultrasound-assisted applications, including CNS modulation and blood-brain barrier permeabilization. The model predicts that the cellular membrane is intrinsically capable of absorbing mechanical energy from the ultrasound field and transforming it into expansions and contractions of the intramembrane space. It further predicts that the maximum area strain is proportional to the acoustic pressure amplitude and inversely proportional to the square root of the frequency (ε A;max ∝ P 0:8 A f − 0:5 ) and is intensified by proximity to free surfaces, the presence of nearby microbubbles in free medium, and the flexibility of the surrounding tissue. Model predictions were experimentally supported using transmission electron microscopy (TEM) of multilayered live-cell goldfish epidermis exposed in vivo to continuous wave (CW) ultrasound at cavitational (1 MHz) and noncavitational (3 MHz) conditions. Our results support the hypothesis that ultrasonically induced bilayer membrane motion, which does not require preexistence of air voids in the tissue, may account for a variety of bioeffects and could elucidate mechanisms of ultrasound interaction with biological tissue that are currently not fully understood.A central hypothesis regarding nonthermal interactions of ultrasound (US) energy and biological tissue is that they are primarily mediated by cavitation, that is, the activity in the US field of gas bubbles generated from submicron-sized gas pockets known as cavitation nuclei: their steady pulsations (stable cavitation) or rapid collapse (inertial cavitation) (1) and their interaction with cells, tissue, and organs (2-4). Nevertheless, this hypothesis has major limitations because low-intensity noncavitational US exposures of <100 mW·cm −2 , spatial peak temporal average (SPTA), have also been shown to induce bioeffects in cells and tissues without evidence of inertial or stable cavitation being present (3-5). On the other hand, whereas the source of in vivo cavitation is not clear, the bilayer membrane seems to be associated with many of the cellular bioeffects at a wide range of US intensities: from excitation of neuronal circuits [3 W·cm −2 spatial peak temporal peak (SPTP), 0.44 MHz] (6) to increased transfection rates in smooth muscle cells (400 mW·cm −2 SPTP, 1 MHz) (7). Our objective here is to introduce a unique hypothesis of direct interaction between the oscillating acoustic pressure and the cellular bilayer memb...

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Section: Discussionmentioning

confidence: 61%

Intramembrane cavitation as a unifying mechanism for ultrasound-induced bioeffects

Krasovitski

Frenkel

Shoham

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

462

375

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“…Furthermore, this nanotheranostic MPH SH ‐PFH as a tailored ultrasound‐guided HIFU synergistic agent has been explored for tumor ablation. Based on ex vivo bovine liver model (Figure S6), the largest HIFU ablated volume after being injected with MPH SH ‐PFH (about 105 mm 3 ), compared to those after being injected with PBS control, MSNCs, MPH SS and MPH SH (about corresponding 59, 68, 59 and 71 mm 3 respectively), demonstrates a much enhanced ablation efficiency owing to its strong mechanical and chemical effects presumably arising from PFH bubble cavitations under this high power exposure (120 W, ISAL of 7225 W/cm 2 ), which can also be found in the previous work …”

Section: Resultsmentioning

confidence: 99%

An Intelligent Nanotheranostic Agent for Targeting, Redox‐Responsive Ultrasound Imaging, and Imaging‐Guided High‐Intensity Focused Ultrasound Synergistic Therapy

Wang

Chen

Zhang

et al. 2013

Small

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A novel multifunctional nanotheranostic agent with targeting, redox-responsive ultrasound imaging and ultrasound imaging-guided high-intensity focused ultrasound (HIFU) therapy (MSNC-PEG-HA(SS)-PFH, abbreviated as MPH(SS)-PFH) capabilities is developed. The redox-responsive guest molecule release and ultrasound imaging functions can be both integrated in such a "smart" theranostic agent, which is accomplished by the redox-triggered transition from the crosslinking state to retrocrosslinking state of the grafted polyethylene glycol-disulfide hyaluronic acid molecules on the particle surface when reaching a reducing environment in vitro. More importantly, under the tailored ultrasound imaging guiding, in vivo Hela tumor-bearing nude mice can be thoroughly and spatial-accurately ablated during HIFU therapy, due to the targeted accumulation, responsive ultrasound imaging guidance and the synergistic ablation functions of nanotheranostic agent MPH(SS)-PFH in the tumors. This novel multifunctional nano-platform can serve as a promising candidate for further studies on oncology therapy, due to its high stability, responsive and indicative ultrasound imaging of tumors, and enhanced HIFU therapeutic efficiency and spatial accuracy under ultrasound-guidance.

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“…HIFU is an ablative technique by converging multiple beams of high‐intensity ultrasound to result in coagulative tissue necrosis in the area 4,5 . HIFU has been used in treating different solid tumors, 2,3 and its use in breast fibroadenoma has been described in only a few European studies (Table 1).…”

Section: Hahn Et Al 2018 Peek Et Al 2016mentioning

confidence: 99%

Prospective clinical trial on high‐intensity focused ultrasound for the treatment of breast fibroadenoma

Kwong¹,

Co²,

Chen³

et al. 2021

Breast J

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Transmission electron microscopy of rabbit liver after high-intensity focused ultrasound ablation combined with ultrasound contrast agents

Cited by 5 publications

References 12 publications

Intramembrane cavitation as a unifying mechanism for ultrasound-induced bioeffects

Intramembrane cavitation as a unifying mechanism for ultrasound-induced bioeffects

An Intelligent Nanotheranostic Agent for Targeting, Redox‐Responsive Ultrasound Imaging, and Imaging‐Guided High‐Intensity Focused Ultrasound Synergistic Therapy

Prospective clinical trial on high‐intensity focused ultrasound for the treatment of breast fibroadenoma

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