Transmission of a cytological heterogeneity from the leaf to the protoplasts in culture

DNA repair synthesis in plant protoplasts is aphidicolin‐resistant

“…Protoplasts were isolated from 2-3 cm long leaves. This stage of development corresponds in this particular species to a good cytological homogeneity, with >90% of Grphase diploid nuclei [21 ]. The isolation procedure implied carborundum brushing, enzymatic digestion and stationary density gradient purification, as in [19].…”

Plant Protoplast Viability

“…This is reflected in the variable capacity to regenerate plants from protoplasts of leaves among different species as well as among tissues (of leaf, hypocotyl, shoot tip) within the same species. Magnien et al (1982) have demonstrated that the cytological heterogeneity of cells of leaf tissues is reflected in the protoplast population in culture. The capacity to regenerate plants from mesophyll protoplasts of oilseed rape and the present incapacity in the case of sugar beet from leaves of similar age is probably a reflection of this variable cytological heterogeneity (cells of sugar beet leaf callus display a high degree of chromosomal aberration).…”

Hybrid genes in the analysis of transformation conditions. 3. Temporal/spatial fate of NPTII gene integration, its inheritance and factors affecting these processes in Nicotiana plumbaginifolia

“…Cytometric and cytological studies [4,12] and experiments of UV mutagenesis [16] in the same species have indicated that more than 90 ~o of the freshly isolated protoplasts were in the Go phase and that the initiation of the first DNA replication (entrance into S I phase) took place 20 to 24 h after isolation and ! culture.…”

“…More specifically, we wished to: -define the integration time and pattern with respect to the first DNA replication cycle(s) in freshly isolated haploid mesophyll protoplasts. Thus, we took advantage of the fact that these cells are blocked in G o [12] and contain the x-set of chromatids, while no experimental synchronization is applied to interfere with the natural course of cell cycle processing; -study the conditions of DNA integration and its transmission to the progeny following treatments known to affect DNA integrity (UV irradiation) or DNA replication (3-BA) by using diploid mesophyll protoplasts.…”