Transmission of Campylobacter coli in chicken embryos

Abstract

Campylobacter coli is an important species involved in human cases of enteritis, and chickens are carriers of the pathogen mainly in developing country. The current study aimed to evaluate the transmission of C. coli and its pathogenic effects in chicken embryos. Breeder hens were inoculated intra-esophageally with C. coli isolated from chickens, and their eggs and embryos were analyzed for the presence of bacteria using real-time PCR and plate culture. The viability of embryos was verified. In parallel, SPF eggs were inoculated with C. coli in the air sac; after incubation, the embryos were submitted to the same analysis as the embryos from breeder hens. In embryos and fertile eggs from breeder hens, the bacterium was only identified by molecular methods; in the SPF eggs, however, the bacterium was detected by both techniques. The results showed no relationship between embryo mortality and positivity for C. coli in the embryos from breeder hens. However, the presence of bacteria is a cause of precocious mortality for SPF embryos. This study revealed that although the vertical transmission is a possible event, the bacteria can not grow in embryonic field samples.

Poultry Gastrointestinal Microbiome Changes During Ontogenesis

“…It is generally believed that the GIT of avian embryos is sterile [1,15,16], and the formation of digestive system microbiocenosis takes place after hatching as due to a contact with the environment [5][6][7]. However, there is evidence obtained by classical microbiology methods [17,18] and real time PCR [19] which indicates the ability of microorganisms to colonize GIT of birds still inside an egg, at the embryonic development stage.…”

“…A high percentage of order Bacillales representatives, mostly bacteria of genus Bacillus, were detected in GIT of embryos. The majority of them are capable of poultry digestive tract colonization, synthesis of organic acids, bacteriocins, antibiotics and enzymes, active participation in metabolic processes of various nutritious substrates [18,19]. Apart from that, several unidentified bacterial genotypes were observed.…”

Efeito da luz ultravioleta na eclodibilidade e viabilidade de pintos provenientes de ovos férteis sujos

“…A incubação de ovos visivelmente sujos, de aves com sintomatologia clínica ou subclínica de doenças que afetam o intestino, postos em ninhos com cama suja ou no piso, não é indicada por razões de biosseguridade, pois pode elevar o risco de contaminação dos pintinhos dentro de uma mesma unidade de incubação por vários microrganismos patogênicos, como os dos gêneros Escherichia, Aspergillus, Pseudomonas, Campylobacter, Salmonella, dentre outros (Rossi et al, 2012;Buhr et al, 2013). A cutícula que envolve o ovo evita que patógenos invadam seu interior através dos poros na casca, e a limpeza dos ovos pode causar a perda de sua integridade.…”

Feed Additive Vinivet of Apicultural Products as an Alternative for Antibiotic Growth Promoters in Broiler Chick Di-Ets — Bactericidal and Biostimulating Effect

“…Thus, it is generally believed that the gastrointestinal tract (GIT) of avian embryos is sterile [9][10][11][12], and the formation of digestive system microbiocenosis occurs after hatching as a result of contacts with the environment [13][14][15]. However, there is evidence obtained by the methods of classical microbiology [16] and using real-time PCR [17] which indicates the ability of microorganisms to colonize the digestive tract of poultry embryos.…”