Transmission of Cucumber Leaf Spot Virus by Olpidium Radicale

Campbell, R. N.; Lecoq, Hervé; Wipf-Scheibel, Catherine; Sim, S. T.

doi:10.1099/0022-1317-72-12-3115

Cited by 24 publications

(21 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The seven viruses were described previously [Campbell et al, 1991]. Partially purified preparations of CLSV, CSBV, PAMV, and SqNV were stored at -20 ~ in 50% glycerol.…”

Section: Virusesmentioning

confidence: 99%

“…The carmo-and tombusviruses share many biophysical characteristics [Martelli et al, 1988;Morris and Carrington, 1988] and may have similar fungal vectors [Campbell et al, 1991]. The carmoviruses found on cucurbit hosts [Morris and Carrington, 1988] include: cucumber soil borne virus (CSBV), cucumber leaf spot virus (CLSV), cucumber fruit streak virus (CLSV-FS) that is serologically related to CLSV [Weber et al, 1986], and melon necrotic spot virus (MNSV).…”

Section: Introductionmentioning

confidence: 99%

“…comm. ] although not in DAS-ELISA [Campbell et al, 1991]. Cucurbit-infecting tombusviruses include cucumber necrosis virus (CNV) that was listed as a carmovirus [Morris and Carrington, 1988] but has been transferred to the tombusvirus group [Rochon and Tremaine, 1988] and petunia asteroid mosaic virus (PAMV) that was isolated from cucumber [Campbell et al, 1990].…”

Section: Introductionmentioning

confidence: 99%

“…: O. radicale Schwartz & Cook fide Lange & Insunza, O. cucurbitacearum Barr & Dias) transmit CNV [Dias, 1970a], MNSV [Furuki, 1981], SqNV [Lin and Palagi, 1983], CLSV and CLSV-FS [Campbell et al, 1991]. Although Gerhardson suggested to Lange and Insunza [1977] that this fungus may be the vector of red clover necrotic mosaic dianthovirus (RCNMV), his experiments have not been reported.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Virus transmission by host-specific strains ofOlpidium bornovanus andOlpidium brassicae

1995

Self Cite

View full text Add to dashboard Cite

Zoospores of 12 isolates O. bornovanus from geographically diverse sites and representing the three host specific cucurbit strains were tested as vectors for seven viruses using watermelon bait plants and the in vitro acquisition method. All isolates of the cucumber, melon, and squash strains transmitted melon necrotic spot carmovirus (MNSV) and cucumber necrosis tombusvirus (CNV) but none transmitted petunia asteroid mosaic tombusvirus (PAMV) or tobacco necrosis necrovirus (TNV). The isolates varied as vectors of three other carmoviruses: cucumber leaf spot virus (CLSV); cucumber soil borne virus (CSBV); and squash necrosis virus (SqNV). All cucumber isolates transmitted CLSV and SqNV but not CSBV. Some of the melon isolates transmitted CLSV and SqNV but none transmitted CSBV. Two squash isolates transmitted CSBV and SqNV but not CLSV. Two isolates of O. brassicae transmitted only TNV and a third did not transmit any of the viruses. The species of bait plant sometimes affected transmission. The most efficient vector strains of O. bornovanus, as determined by reducing zoospores and virus in the inoculum, were the cucumber strain for CLSV; the cucumber strain for CNV if cucumber was the bait plant or melon strain if watermelon was the bait plant; and the squash strain for SqNV. The plurivorous strain of O. brassicae was the most efficient vector of TNV. Olpidium bornovanus is the first vector reported for CSBV and is confirmed as a vector of SqNV. It is proposed that all carmoviruses may have fungal vectors. Ligniera sp. did not transmit any of the viruses in one attempt.Abbreviations: CLSV --cucumber leaf spot virus; CNV --cucumber necrosis virus; CSBV = cucumber soil borne virus; MNSV = melon necrotic spot virus; PAMV --petunia asteroid mosaic virus; SqNV = squash necrosis virus; TNV = tobacco necrosis virus; TBSV --tomato bushy stunt virus.

show abstract

“…The seven viruses were described previously [Campbell et al, 1991]. Partially purified preparations of CLSV, CSBV, PAMV, and SqNV were stored at -20 ~ in 50% glycerol.…”

Section: Virusesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Virus transmission by host-specific strains ofOlpidium bornovanus andOlpidium brassicae

1995

Self Cite

View full text Add to dashboard Cite

show abstract

“…Purified virions were tested for transmission by O. bornovanus zoospores as previously described (5,7,20). Virus (1 g) was incubated with 10 ml of zoospores (10 4 /ml in 50 mM glycine, pH 7.6).…”

Section: Methodsmentioning

confidence: 99%

Identification of Specific Cucumber Necrosis Virus Coat Protein Amino Acids Affecting Fungus Transmission and Zoospore Attachment

Kakani

Sgro

Rochon

2001

J Virol

View full text Add to dashboard Cite

Cucumber necrosis virus (CNV) is naturally transmitted in the soil by zoospores of the fungal vectorOlpidium bornovanus. Successful transmission requires that virus particles attach to the surface of zoospores prior to zoospore encystment on host roots. Mechanically passaged CNV was screened for mutants deficient in fungus transmission. We found six such mutants, exhibiting transmission efficiencies ranging from approximately 14 to 76% of that of wild-type (WT) CNV. Results of in vitro virus-zoospore binding assays show that each mutant binds to zoospores less efficiently than WT CNV (21 to 68%), suggesting that defects in transmission for these mutants are at least partially due to inefficient zoospore binding. Analysis of the structure of the CNV coat protein subunit and trimer indicates that affected amino acids in all of the mutants are located in the shell or protruding domain and that five of six of them are potentially exposed on the surface of the virus particle. In addition, several of the mutated sites, along with a previously identified site in a Efficient transmission of the majority of plant viruses requires distinct invertebrate or fungal vectors. In most cases, transmission has been shown to be a highly specific process in which only certain vectors can transmit certain viruses (for reviews, see references 4, 6, 13, 14, 23, and 35). These observations suggest that virus particles as well as vectors contain specific sites that mediate their recognition. The coat protein (CP) of a plant virus has been shown to play an important role in transmission, and particular amino acids within the CP have been shown to be essential for this process (for reviews, see references 4, 6, 13, 14, 23, and 35). However, for the most part, the exact role of these amino acids in transmission including their potential role in vector attachment, is not known. Recent work with cucumber necrosis virus (CNV) has suggested that attachment of virions to vector zoospores is an important aspect of the transmission process (24).CNV, a member of the genus Tombusvirus, is a 30-nm spherical virus with a monopartite positive-sense RNA genome (25). Transmission of CNV in nature occurs via zoospores of the Chytridiomycete fungus, Olpidium bornovanus (6, 9, 24). Zoospores and virus particles are released independently into the soil from the roots of infected plants. Virus is adsorbed onto the plasma membrane of zoospores and then enters into roots upon zoospore encystment. Studies of CNV transmission by O.bornovanus, and Olpidium transmission of several other small spherical plant viruses, have shown that the transmission process is highly specific (1, 6). For example, O. bornovanus transmits CNV but not Tobacco necrosis necrovirus (TNV), and conversely, O. brassicae transmits TNV but not CNV (10, 34). Moreover, different isolates of O. bornovanus transmit different viruses with varying efficiency (7), and different strains of TNV are transmitted with varying efficiency by the same O. brassicae isolate (17,33,34). Electron microscopy st...

show abstract

Fungal Transmission of Plant Viruses

Rochon

2009

CP Microbiology

View full text Add to dashboard Cite

Fungal zoospores of Olpidium species transmit several viruses in the family Tombusviridae as well as in the Ophio‐ and Varicosavirus genera. This unit describes procedures for virus transmission by Olpidium sp. The method is useful for assessing fungal transmissibility of a given virus as well as for further studies on molecular and biological aspects of virus/vector interaction. Curr. Protoc. Microbiol. 12:16B.4.1‐16B.4.17. © 2009 by John Wiley & Sons, Inc.

show abstract

Transmission of Cucumber Leaf Spot Virus by Olpidium Radicale

Cited by 24 publications

References 12 publications

Virus transmission by host-specific strains ofOlpidium bornovanus andOlpidium brassicae

Virus transmission by host-specific strains ofOlpidium bornovanus andOlpidium brassicae

Identification of Specific Cucumber Necrosis Virus Coat Protein Amino Acids Affecting Fungus Transmission and Zoospore Attachment

Fungal Transmission of Plant Viruses

Contact Info

Product

Resources

About