Transmission of dengue virus from a donor to a recipient after living donor liver transplantation

Saigal, D M Sanjiv; Choudhary, Narendra S.; Saraf, Neeraj; Kataria, Sushila; Mohanka, Ravi; Soin, Arvinder S.

doi:10.1002/lt.23755

Cited by 39 publications

(25 citation statements)

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“…In addition, DENV RNA or antigen has been found in the liver of patients who succumbed to DHF/DSS (141–143). Recently, a case of DENV transmission following a liver transplant has been reported (144). However, the cellular localization of DENV replication in the liver is still controversial, and possibly deserves more attention in future human studies.…”

Section: Mouse Studies To Complement Human Studiesmentioning

confidence: 99%

Mouse Models to Study Dengue Virus Immunology and Pathogenesis

Zellweger

Shresta

2014

Front. Immunol.

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The development of a compelling murine model of dengue virus (DENV) infection has been challenging, because DENV clinical isolates do not readily replicate or cause pathology in immunocompetent mice. However, research using immunocompromised mice and/or mouse-adapted viruses allows investigation of questions that may be impossible to address in human studies. In this review, we discuss the potential strengths and limitations of existing mouse models of dengue disease. Human studies are descriptive by nature; moreover, the strain, time, and sequence of infection are often unknown. In contrast, in mice, the conditions of infection are well defined and a large number of experimental parameters can be varied at will. Therefore, mouse models offer an opportunity to experimentally test hypotheses that are based on epidemiological observations. In particular, gain-of-function or loss-of-function models can be established to assess how different components of the immune system (either alone or in combination) contribute to protection or pathogenesis during secondary infections or after vaccination. In addition, mouse models have been used for pre-clinical testing of anti-viral drugs or for vaccine development studies. Conclusions based on mouse experiments must be extrapolated to DENV-infection in humans with caution due to the inherent limitations of animal models. However, research in mouse models is a useful complement to in vitro and epidemiological data, and may delineate new areas that deserve attention during future human studies.

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Section: Mouse Studies To Complement Human Studiesmentioning

confidence: 99%

Mouse Models to Study Dengue Virus Immunology and Pathogenesis

Zellweger

Shresta

2014

Front. Immunol.

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“…Traditionally, DENV is transmitted to humans via the bite of the infectious female mosquitos of the genus Aedes , mainly A. aegypti . Recently, nonvector DENV transmission has been reported in the literature, including intravenous and/or intradermal inoculation, mucocutaneous exposure, blood transfusion, hematopoietic stem cell transplantation (HSCT), solid organ transplantation, needlestick in patient care or laboratory accident, vertical transmission from mother to infant, and breastfeeding . This line of evidence suggests that DENV can be transmitted through direct exposure through blood/body fluid exposure, leading to disease.…”

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confidence: 99%

Identification and characterization of permissive cells to dengue virus infection in human hematopoietic stem and progenitor cells

Hsu

Lai

et al. 2019

Transfusion

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BACKGROUND Dengue virus (DENV) is a significant threat to public health in tropical and subtropical regions, where the frequency of human migration is increasing. Transmission of DENV from donors to recipients after hematopoietic stem cell transplantation has been steadily described. However, the underlying mechanisms remain unclear. STUDY DESIGN AND METHODS Freshly isolated bone marrow (BM) was subjected to DENV infection, followed by multicolor fluorescence‐activated cell sorting (FACS) analysis. Virus in supernatants was collected and analyzed by plaque assay. RESULTS DENV‐1 to DENV‐4 could effectively infect freshly obtained BM and produced infectious virus. DENV infection did not change the quantitative population of hematopoietic stem and progenitor cells (HSPCs), megakaryocytic progenitor cells (MkPs) and megakaryocytes. Additionally, DENV antigen, nonstructural protein 1, was enriched in HSPCs and MkPs of DENV infected marrow cells. CD34+, CD133+, or CD61+ cells sorted out from BM were not only the major contributing targets facilitating the DENV infection directly but also facilitated the spread of DENV into other cells when cocultured. CONCLUSION Results suggest that DENV can efficiently infect HSPCs, which might jeopardize the recipients if DENV‐infected cells were subsequently used. We therefore raise the need for DENV screening for both the donors and recipients of hematopoietic stem cell transplantation, especially for donors exposed to endemic areas, to mitigate DENV infection in immunocompromised recipients.

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“…Donor-derived transmission of other mosquito-borne viruses with similar epidemiologic and biologic features, notably dengue virus and West Nile virus, has been documented (9,10). Although no cases of CHIKV transmission by transplantation have yet been reported, studies have shown that CHIKV can be isolated from corneas of acutely infected individuals (11), and atypical manifestations of CHIKV infection were reported in a recipient who became infected 7 years after receiving a liver transplant (12).…”

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confidence: 99%

Evaluation of Two Enzyme-Linked Immunosorbent Assay Kits for Chikungunya Virus IgM Using Samples from Deceased Organ and Tissue Donors

Prince¹,

Altrich²,

Nowicki³

2016

Clin Vaccine Immunol

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The identification of nearly 3,500 cases of chikungunya virus (CHIKV) infection in U.S. residents returning in 2014 and 2015 from areas in which it is endemic has raised concerns within the transplant community that, should recently infected individuals become organ and/or tissue donors, CHIKV would be transmitted to transplant recipients. Thus, tests designed to detect recent CHIKV infection among U.S. organ and tissue donors may become necessary in the future. Accordingly, we evaluated 2 enzymelinked immunosorbent assays (ELISAs) for CHIKV IgM readily available in the United States using 1,000 deidentified serum or plasma specimens collected from donors between November 2014 and March 2015. The Euroimmun indirect ELISA identified 38 reactive specimens; however, all 38 were negative for CHIKV IgG and IgM in immunofluorescence assays (IFAs) conducted at a reference laboratory and, thus, were falsely reactive in the Euroimmun CHIKV IgM assay. The InBios IgM-capture ELISA identified 26 reactive samples, and one was still reactive (index > 1.00) when retested using the InBios kit with a background subtraction modification to identify false reactivity. This reactive specimen was CHIKV IgM negative but IgG positive by IFAs at two reference laboratories; plaque reduction neutralization testing (PRNT) demonstrated CHIKV-specific reactivity. The IgG and PRNT findings strongly suggest that the InBios CHIKV IgM-reactive result represents true reactivity, even though the IgM IFA result was negative. If testing organ/tissue donors for CHIKV IgM becomes necessary, the limitations of the currently available CHIKV IgM ELISAs and options for their optimization must be understood to avoid organ/tissue wastage due to falsely reactive results. C hikungunya virus (CHIKV) is an alphavirus transmitted from person to person via mosquito bites (1). Symptoms include fever, rash, and debilitating arthralgia; 15% to 60% of patients develop chronic arthralgia leading to arthritic joint damage (2). After a large CHIKV outbreak in India and southeast Asia in 2004 through 2006, in which nearly 2 million people became infected (3, 4), epidemiologists predicted that CHIKV might move to other geographic areas where the mosquito vectors are found (5). This prediction was realized in December 2013, when local transmission of CHIKV was reported on the Caribbean island of St. Martin (6). CHIKV infection has since spread throughout the Caribbean basin (7) and is now also endemic in Mexico, Central America, and South America and in the Caribbean island nations. In conjunction with this outbreak, 3,490 cases in U.S. residents (from 49 of 50 states) were reported to the CDC during 2014 and 2015; 3,478 cases represented infections acquired during international travel to areas where CHIKV is endemic, whereas 12 cases represented local transmission (8).There is concern within the transplant community that CHIKV could be transmitted from organ and/or tissue donors to recipients. Donor-derived transmission of other mosquito-borne viruses with similar epidemi...

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Transmission of dengue virus from a donor to a recipient after living donor liver transplantation

Cited by 39 publications

References 2 publications

Mouse Models to Study Dengue Virus Immunology and Pathogenesis

Mouse Models to Study Dengue Virus Immunology and Pathogenesis

Identification and characterization of permissive cells to dengue virus infection in human hematopoietic stem and progenitor cells

Evaluation of Two Enzyme-Linked Immunosorbent Assay Kits for Chikungunya Virus IgM Using Samples from Deceased Organ and Tissue Donors

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