Transmission of <i>Zucchini yellow mosaic virus</i> by Colonizing and Non‐colonizing Aphids in Greece and New Aphid Species Vectors of the Virus

Katis, N. I.; Tsitsipis, J. A.; Lykouressis, D. P.; Papapanayotou, A.; Margaritopoulos, John T.; Kokinis, G.; Perdikis, D.; Manoussopoulos, I. N.

doi:10.1111/j.1439-0434.2006.01096.x

Cited by 68 publications

(46 citation statements)

References 30 publications

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“…ZYMV is horizontally transmitted in a nonpersistent manner by at least 26 aphid species (12). Transmission occurs as a result of an interaction between the stylet of the aphid, the helper component protein (HC-Pro), and the conserved DAG (AspAla-Gly) region of the coat protein (CP) (18).…”

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confidence: 99%

Experimental Verification of Seed Transmission of Zucchini yellow mosaic virus

Simmons

Holmes

Gildow³

et al. 2011

Plant Disease

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Simmons, H. E., Holmes, E. C., Gildow, F. E., Bothe-Goralczyk, M. A., and Stephenson, A. G. 2011. Experimental verification of seed transmission of Zucchini yellow mosaic virus. Plant Dis. 95:751-754.Within two decades of its discovery, Zucchini yellow mosaic virus (ZYMV) achieved a global distribution. However, whether or not seed transmission occurs in this economically significant crop pathogen is controversial, and the relative impact of seed transmission on the epidemiology of ZYMV remains unclear. Using reverse transcriptionpolymerase chain reaction, we observed a seed transmission rate of 1.6% in Cucurbita pepo subsp. texana and show that seed-infected C. pepo plants are capable of initiating horizontal ZYMV infections, both mechanically and via an aphid vector (Myzus persicae). We also provide evidence that ZYMV-infected seeds may act as effective viral reservoirs, partially accounting for the current geographic distribution of ZYMV. Finally, the observation that ZYMV infection of C. pepo seeds results in virtually symptomless infection, coupled with our finding that an antibody test failed to detect vertically transmitted ZYMV in infected seed, highlights the urgent need to standardize current detection methods for seed infection.Since the discovery of Zucchini yellow mosaic virus (ZYMV) in Italy in 1973, and its subsequent description in 1981 (14), this emerging RNA virus has spread rapidly and achieved an effectively global distribution (5). Although a number of explanations have been put forward to account for the widespread geographic distribution and persistence of this virus, including the international trading of infected fruit, plants, or seeds, as well as overwintering in alternative hosts and noncolonizer aphids, the mechanisms underlying the rapid dissemination and persistence of ZYMV remain unclear (13). ZYMV is a single-stranded positive-sense RNA virus of the family Potyviridae that can result in yellowing and stunting of the plant, as well as severe leaf and fruit deformities that can reduce yields up to 94% (1). Given that cucurbit (squash, melon, and cucumber) production in the United States alone is estimated to be worth approximately $1.5 billion per year (2), the economic significance of this crop pathogen is enormous. Understanding the epidemiology and evolution of ZYMV is therefore central to controlling this devastating crop disease.Viral transmission generally occurs in one of two ways: horizontally, which is the transmission of the virus between unrelated hosts, or vertically, which is the transmission of the virus from parent to offspring. ZYMV is horizontally transmitted in a nonpersistent manner by at least 26 aphid species (12). Transmission occurs as a result of an interaction between the stylet of the aphid, the helper component protein (HC-Pro), and the conserved DAG (AspAla-Gly) region of the coat protein (CP) (18). However, the current worldwide distribution of ZYMV is unlikely to have resulted from aphid transmission alone, particularly as the aphid vector remains virul...

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confidence: 99%

Experimental Verification of Seed Transmission of Zucchini yellow mosaic virus

Simmons

Holmes

Gildow³

et al. 2011

Plant Disease

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“…The primary mode of transmission is via aphids in a non-persistent manner. Although 10 aphid species have been reported as vectors (Katis et al, 2006;Lisa et al, 1981), a wider range of potential aphid vectors has been identified under experimental conditions (Blackman & Eastop, 2000;Katis et al, 2006). While aphid transmission is undoubtedly the main route of spread for ZYMV, infrequent seed transmission has also been proposed (Robinson et al, 1993;Schrijnwerkers et al, 1991), the epidemiological importance of which is uncertain (Johansen et al, 1994).…”

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Rapid evolutionary dynamics of zucchini yellow mosaic virus

Simmons

Holmes

Stephenson

2008

Journal of General Virology

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Zucchini yellow mosaic virus (ZYMV) is an economically important virus of cucurbit crops. However, little is known about the rate at which this virus has evolved within members of the family Cucurbitaceae, or the timescale of its epidemiological history. Herein, we present the first analysis of the evolutionary dynamics of ZYMV. Using a Bayesian coalescent approach we show that the coat protein of ZYMV has evolved at a mean rate of 5.0¾10"4 nucleotide substitutions per site, per year. Notably, this rate is equivalent to those observed in animal RNA viruses. Using the same approach we show that the lineages of ZYMV sampled here have an ancestry that dates back no more than 800 years, suggesting that human activities have played a central role in the dispersal of ZYMV. Finally, an analysis of phylogeographical structure provides strong evidence for the in situ evolution of ZYMV within individual countries.

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“…The coat protein (CP) has a molecular weight of 36 kDa (Lisa and Lecoq, 1984;Adams et al, 2012). ZYMV is experimentally transmitted by 26 species of aphids in a non-persistent manner (Katis et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus

Spadotti

Wassano

Rezende

et al. 2015

Sci. agric. (Piracicaba, Braz.)

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Zucchini yellow mosaic virus (ZYMV) causes substantial economic losses in cucurbit crops. Although ZYMV has been present in Brazil for more than 20 years, there is little information about the biological and molecular characteristics of the isolates found in the country. This study aimed to characterize the experimental hosts, pathotypes and genetic diversity of a collection of eleven Brazilian ZYMV isolates within the coat protein gene. For biological analysis, plant species from Amaranthaceae, Chenopodiaceae, Cucurbitaceae, Fabaceae, Solanaceae, and Pedaliaceae were mechanically inoculated and pathotypes were identified based on the reaction of a resistant Cucumis melo, accession PI414723. All of the cucurbit species/varieties and Sesamum indicum were systemically infected with all isolates. The nucleotide sequence variability of the coat protein gene ranged from 82 % to 99 % compared to the corresponding sequences of ZYMV isolates from different geographical locations. No recombination event was detected in the coat protein gene of the isolates.

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Transmission of Zucchini yellow mosaic virus by Colonizing and Non‐colonizing Aphids in Greece and New Aphid Species Vectors of the Virus

Cited by 68 publications

References 30 publications

Experimental Verification of Seed Transmission of Zucchini yellow mosaic virus

Experimental Verification of Seed Transmission of Zucchini yellow mosaic virus

Rapid evolutionary dynamics of zucchini yellow mosaic virus

Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus

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