Transmission of methicillin-resistant Staphylococcus intermedius between humans and animals

Duijkeren, Engeline van; Houwers, D.J.; Schoormans, A.; Broekhuizen-Stins, M.J.; Ikawaty, Risma; Fluit, Ad C.; Wagenaar, Jaap A.

doi:10.1016/j.vetmic.2007.11.016

Cited by 65 publications

(50 citation statements)

References 11 publications

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“…29 Human infections with S. intermedius and S. pseudintermedius have also been identified in food poisoning cases, catheter-related bacteremia, ischemic cardiomyopathy, and ventricular tachycardia cases, 3,7,16,25,28 and direct transmission between animals and human beings has also been demonstrated. 15,27 Such characteristics emphasize their potential as zoonotic pathogens. Furthermore, increasing evidence has identified the emergence and spread of methicillin-resistant S. intermedius (MRSI) and S. pseudintermedius (MRSP) isolates.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Antimicrogram of Staphylococcus Intermedius Group Isolates from Veterinary Staff, Companion Animals, and the Environment in Veterinary Hospitals in Korea

et al. 2011

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Abstract. The Staphylococcus intermedius bacterial group (SIG) includes 3 distinct genetically heterogenous species: S. intermedius, S. pseudintermedius, and S. delphini. This pathogen group is associated with many opportunistic skin and ear infections in companion animals. Human infections with S. intermedius and S. pseudintermedius isolates and the emergence of methicillin-resistant isolates have been recently reported, which emphasizes the importance of nationwide identification of SIG isolate prevalence and antibiotic resistance in veterinary clinics. In the present study, a total of 178 SIG isolates were obtained from veterinary staff (n 5 40), companion animals (n 5 115), and the local environment (n 5 23) in 8 Korean veterinary hospitals. Isolates were differentiated into 167 S. pseudintermedius (93.8%) and 11 S. intermedius (6.2%) isolates; S. delphini isolates were not identified. The most effective antibiotics against these isolates included amoxicillin-clavulanic acid, amikacin, nitrofloxacin, imipenem, and vancomycin; whereas ampicillin, penicillin, tetracycline, erythromycin, and trimethoprim-sulfamethoxazole were not effective. Surprisingly, the 128 SIG isolates (71.9%) displayed multiple drug resistance (MDR) against 3 or more antibiotic classes. Out of 52 SIG isolates carrying the methicillin-resistance gene (mecA), only 34 (65.4%) were oxacillin-resistant, and 49 (94.2%) methicillin-resistant SIG were multidrug resistant. This finding suggests the presence of greater numbers of MDR phenotypes than other isolates (P , 0.05).

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Section: Introductionmentioning

confidence: 99%

Prevalence and Antimicrogram of Staphylococcus Intermedius Group Isolates from Veterinary Staff, Companion Animals, and the Environment in Veterinary Hospitals in Korea

et al. 2011

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“…Staphylococcus pseudintermedius rather than S. intermedius is the opportunistic species of the SIG that colonizes and causes infections in dogs and cats frequently. Beside this, S. pseudintermedius could be transferred from infected and/ or colonized pets to humans, which causes an increasing public health concern [1] . Staphylococcus pseudintermedius is occasionally isolated from serious human infections, particularly from people who are in close contact with pets, such as small animal veterinarians and pet owners [2][3][4][5] .…”

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confidence: 99%

Methicillin Resistance in Staphylococcus pseudintermedius Isolated from Shelter Dogs in Turkey

Sareyyüpoğlu¹,

Müştak²,

Cantekin³

et al. 2014

Kafkas Univ Vet Fak Derg

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SummaryStaphylococcus pseudintermedius is a recently described species of staphylococci that is frequently isolated from dogs. The increase in antimicrobial resistance in staphylococci and transfer of S. pseudintermedius from infected pets to humans threaten the public health worldwide. In this study, it was aimed to determine the prevalence of S. pseudintermedius from skin infections of dogs and to determine the prevalence of methicillin-resistance in S. pseudintermedius isolates by phenotypic and genotypic methods. A total of 41 staphylococci were isolated from 53 dogs. Thirty three (80.4%) staphylococci were identified as S. pseudintermedius by PCR-RFLP analysis of pta gene. Methicillin resistance was also identified in 11 (33.3%) of these isolates by inoculation on chromogenic chromID MRSA agar, oxacillin disc diffusion method and the determination of mecA gene by PCR. This is the sole report in Turkey that described the situation of methicillin resistant S. pseudintermedius. Keywords: Dog, Staphylococcus pseudintermedius, Methicillin resistance Türkiye'deki Barınak Köpeklerinden İzole Edilen Staphylococcus pseudintermedius'larda Metisilin Direnci ÖzetStaphylococcus pseudintermedius, stafilokoklar içerisinde yeni tanımlanmış bir tür olup köpeklerden sıklıkla izole edilmektedir. Stafilokoklarda artan antimikrobiyal direnç ve S. pseudintermedius'un enfekte petlerden insanlara geçmesi, halk sağlığını tehdit etmektedir. Bu çalışmada, köpek deri enfeksiyonlarından izole edilen S. pseudintermedius'ların ve metisilin direncinin prevalansının fenotipik ve genotipik metotlar ile belirlenmesi amaçlanmıştır. Toplam 53 köpekten 41 stafilokok izole edildi. Stafilokok izolatlarının 33'ü (%80.4) pta geninin PCR-RFLP ile analiziyle S. pseudintermedius olarak belirlendi. Metisilin direnci kromogenik chromID MRSA agar, oxacillin disk difüzyon metodu ve mecA geninin belirlendiği PCR yöntemi ile bu izolatların 11'inde (%33.3) tespit edildi. Bu çalışma, Türkiye'de metisilin dirençli S. pseudintermedius'ların durumunu gösteren ilk bilimsel rapordur.

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“…Staphylococcal strains are isolated from various food products in which they are involved in fermentation (18,29). Staphylococcus species can cause a wide variety of diseases in humans and other animals (2,22,(30)(31)(32)35). S. aureus is a major pathogen in human infections (31).…”

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Identification of a Variety of Staphylococcus Species by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

et al. 2010

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Whole-cell fingerprinting by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) in combination with a dedicated bioinformatic software tool (MALDI Biotyper 2.0) was used to identify 152 staphylococcal strains corresponding to 22 staphylococcal species. Spectra of the 152 isolates, previously identified at the species level using a sodA gene-based oligonucleotide array, were analyzed against the main spectra of 3,030 microorganisms. A total of 151 strains out of 152 (99.3%) were correctly identified at the species level; only one strain was identified at the genus level. The MALDI-TOF MS method revealed different clonal lineages of Staphylococcus epidermidis that were of either human or environmental origin, which suggests that the MALDI-TOF MS method could be useful in the profiling of staphylococcal strains. The topology of the dendrogram generated by the MALDI Biotyper 2.0 software from the spectra of 120 Staphylococcus reference strains (representing 36 species) was in general agreement with that inferred from the 16S rRNA gene-based analysis. Our findings indicate that the MALDI-TOF MS technology, associated with a broad-spectrum reference database, is an effective tool for the swift and reliable identification of Staphylococci.

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Transmission of methicillin-resistant Staphylococcus intermedius between humans and animals

Cited by 65 publications

References 11 publications

Prevalence and Antimicrogram of Staphylococcus Intermedius Group Isolates from Veterinary Staff, Companion Animals, and the Environment in Veterinary Hospitals in Korea

Prevalence and Antimicrogram of Staphylococcus Intermedius Group Isolates from Veterinary Staff, Companion Animals, and the Environment in Veterinary Hospitals in Korea

Methicillin Resistance in Staphylococcus pseudintermedius Isolated from Shelter Dogs in Turkey

Identification of a Variety of Staphylococcus Species by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

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