Transmission of Panulirus argus virus 1 (PaV1) and its effect on the survival of juvenile Caribbean spiny lobster

Butler, Mark J.; Behringer, Donald C.; Shields, Jeffrey D.

doi:10.3354/dao01899

Cited by 62 publications

(52 citation statements)

References 41 publications

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“…In the laboratory, the rate of contact transmission of PaV1 was found to be 5 times as high as the rate of water-borne transmission (Butler et al 2008). Yet, in our study, the lowest levels of clinical prevalence of PaV1 in all sampling seasons and in all lobster benthic phases occurred in Vigía Chico, where the highest density of lobsters per casita and the highest measures of aggregation in ND+D casitas also occurred.…”

Section: Sunlight/uv Exposure May Reduce Disease Prevalence In Vigía mentioning

confidence: 99%

“…Moreover, shelter-sharing individuals use their long, spiny antennae in concert to fend off approaching enemies ('group defense behavior'; Herrnkind et al 2001), thus increasing per capita survival. PaV1 can be transmitted by contact, by ingestion of infected tissues, and through water (Butler et al 2008). However, in the laboratory, lobsters were found to be able to detect and avoid shelters occupied by diseased conspecifics; similarly, in Florida Bay, most visibly infected lobsters were found alone (94% solitary) in individual shelters (mostly large sponges and solution holes) (Behringer et al 2006, Behringer & Butler 2010.…”

Section: Introductionmentioning

confidence: 99%

“…Highly infected lobsters become lethargic and eventually die from metabolic waste (Shields 2011). To date, no other hosts or reservoirs for PaV1 have been identified (Butler et al 2008, Shields 2011.…”

Section: Introductionmentioning

confidence: 99%

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Influence of local habitat features on disease avoidance by Caribbean spiny lobsters in a casita-enhanced bay

et al. 2012

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In Bahía de la Ascensión, Mexico, 'casitas' (large artificial shelters) are extensively used to harvest Caribbean spiny lobsters Panulirus argus. After the discovery of a pathogenic virus, Panulirus argus virus 1 (PaV1), in these lobsters, laboratory experiments revealed that PaV1 could be transmitted by contact and through water, and that lobsters avoided shelters harboring diseased conspecifics. To examine these issues in the context of casitas, which typically harbor multiple lobsters of all sizes, we examined the distribution and aggregation patterns of lobsters in the absence/presence of diseased conspecifics (i.e. visibly infected with PaV1) in 531 casitas distributed over 3 bay zones, 1 poorly vegetated ('Vigía Chico', average depth: 1.5 m) and 2 more extensively vegetated ('Punta Allen': 2.5 m; 'Los Cayos': 2.4 m). All zones had relatively high indices of predation risk. Using several statistical approaches, we found that distribution parameters of lobsters were generally not affected by the presence of diseased conspecifics in casitas. Interestingly, however, in the shallower and less vegetated zone (Vigía Chico), individual casitas harbored more lobsters and lobsters were actually more crowded in casitas containing diseased conspecifics, yet disease prevalence was the lowest in lobsters of all sizes. These results suggest that (1) investment in disease avoidance by lobsters is partially modulated by local habitat features, (2) contact transmission rates of PaV1 may be lower in nature than in the laboratory, and (3) water-borne transmission rates may be lower in shallow, poorly vegetated habitats more exposed to solar ultraviolet radiation, which can damage viral particles.

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Section: Sunlight/uv Exposure May Reduce Disease Prevalence In Vigía mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of local habitat features on disease avoidance by Caribbean spiny lobsters in a casita-enhanced bay

et al. 2012

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“…A notable exception is the growing literature on virus PaV1, found in the Caribbean spiny lobster Panulirus argus (e.g. Shields & Behringer 2004, Butler et al 2008.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical properties of double-stranded RNA used to discover a reo-like virus from blue crab Callinectes sapidus

Bowers¹,

Messick²,

Hanif³

et al. 2010

Dis. Aquat. Org.

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Mortality among blue crab Callinectes sapidus in soft shell production facilities is typically 25% or greater. The harvest, handling, and husbandry practices of soft shell crab production have the potential to spread or exacerbate infectious crab diseases. To investigate the possible role of viruses in soft shell crab mortalities, we took advantage of the physicochemical properties of doublestranded RNA (dsRNA) to isolate a putative virus genome. Further characterization confirmed the presence of a reo-like virus that possesses 12 dsRNA genome segments. The virus was present in > 50% of dead or dying soft shell crabs, but fewer than 5% of healthy hard crabs. Injection of the virus caused mortality and resulted in the appearance of viral RNA and virus inclusions in hemocytes. The genome of the virus was partially sequenced and the information used to develop a reverse transcription polymerase chain reaction (RT-PCR) assay that is able to detect the virus genome in as little as 7.5 pg of total RNA. The molecular tools developed during this study will allow us to quantify prevalence of the blue crab reo-like virus in captive (soft shell facilities, aquaculture operations) and wild populations and facilitate understanding of the role this virus has in blue crab life history.

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“…The discovery that PaV1 can be transmitted by contact and over short distances through water (Butler et al 2008) has raised concern about the potential impact of this viral disease on the fisheries for Panulirus argus, especially where casitas are used (Behringer et al 2012). On the other hand, grossly normal lobsters tend to avoid shelters harboring diseased conspecifics, potentially limiting the spread of the virus (Behringer et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Variability in clinical prevalence of PaV1 in Caribbean spiny lobsters occupying commercial casitas over a large bay in Mexico

et al. 2012

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In Bahía de la Ascensión, Mexico, the fishery for spiny lobsters Panulirus argus is based on the extensive use of casitas, large artificial shelters that can harbor the full size range of these highly gregarious lobsters. The discovery of a pathogenic virus in these lobsters (Panulirus argus virus 1, or PaV1) has raised concern about its potential effects on casita-based fisheries. Because in Bahía de la Ascensión visibly infected lobsters represent an immediate loss of revenue, we examined variability in clinical prevalence of PaV1 (percentage of lobsters visibly infected) in thousands of lobsters sampled from the commercial catch at the onset of 3 consecutive fishing years, and from 530 casitas distributed over 3 zones within the bay during 2 fishing and 2 closed seasons. In the commercial catch (lobsters 67 to 147 mm carapace length [CL]), clinical prevalence of PaV1 was low and was not affected by year or sex. In lobsters (9.2 to 115.0 mm CL) that occupied casitas, clinical prevalence of PaV1 varied with sampling season and was always higher in juveniles than in subadults or adults, but was consistently lower in one zone relative to the other 2 zones. The average clinical prevalence of PaV1 in this bay was statistically similar to the average clinical prevalence reported in Cuba, where casitas are also used, and in Florida Bay, USA, where casitas are not used. To date, PaV1 has had no discernible impact on the lobster fishery in Bahía de la Ascensión, suggesting that clinical prevalence is not influenced by the use of casitas per se.

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Transmission of Panulirus argus virus 1 (PaV1) and its effect on the survival of juvenile Caribbean spiny lobster

Cited by 62 publications

References 41 publications

Influence of local habitat features on disease avoidance by Caribbean spiny lobsters in a casita-enhanced bay

Influence of local habitat features on disease avoidance by Caribbean spiny lobsters in a casita-enhanced bay

Physicochemical properties of double-stranded RNA used to discover a reo-like virus from blue crab Callinectes sapidus

Variability in clinical prevalence of PaV1 in Caribbean spiny lobsters occupying commercial casitas over a large bay in Mexico

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