Transmission of the Aegilops ovata chromosomes carrying gametocidal factors in hexaploid triticale (×Triticosecale Wittm.) hybrids

Abstract

The main aim of this work was to induce the chromosome rearrangements between Aegilops ovata (UUMM) and hexaploid triticale (AABBRR) by expression of the gametocidal factor located on the chromosome 4M. The Aegilops ovata × Secale cereale (UUMMRR) amphiploids and triticale ‘Moreno’ were used to produce hybrids by reciprocal crosses. Chromosome dynamics was observed in subsequent generations of hybrids during mitotic metaphase of root meristems and first metaphase of meiosis of pollen mother cells. Chromosomes were identified by genomic in situ hybridisation (GISH) and fluorescence in situ hybridisation (FISH) using pTa71, pTa791, pSc119.2 and pAs1 DNA probes. It has been shown that the origin of the genetic background had an influence on Aegilops chromosome transmission. Moreover, it has been reported that the preferential transmission of chromosome 4M appeared during both androgenesis and gynogenesis. It is also hypothesised that the expression of the triticale Gc gene suppressor had an influence on the semi-fertility of hybrids but did not inhibit the chromosome rearrangements. This paper also describes the double haploid production, which enabled to obtain plants with two identical copies of triticale chromosomes with translocations of Aegilops chromatin segments.

Gametocidal Factor Transferred from Aegilops geniculata Roth Can Be Adapted for Large-Scale Chromosome Manipulations in Cereals

“…geniculata hybrids, which were previously generated by Kwiatek et al (2016c). A GISH analysis of meiosis of PMCs showed that chromosome 4M g was present as a univalent at prophase I (Figure 1b) and metaphase I of meiosis (Figure 1c).…”

“…geniculata , which was analyzed using wheat (Chinese Spring) monosomic 4M g addition plants. Our research team has presented its preferential transmission and gametocidal properties in triticale (Kwiatek et al, 2016c). An investigation of monosomic Triticum aestivum-Ae.…”

“…Seeds of monosomic addition hexaploid triticale plants, carrying a 4M g chromosome from Aegilops geniculata (Kwiatek et al, 2016c), were germinated on wet filter paper in the dark at room temperature for 4 days. Thereafter the plantlets were transferred to soil and cultivated for 6–8 weeks under short-day conditions (8 h light/16 h dark, 20°/18°C).…”

“…Blocking DNA from T. durum (AABB, Duromax) or triticale (AABBRR, Moreno) was sheared by boiling for 30–45 min and used at a ratio of 1:50 (probe:block). Genomic in situ hybridization (GISH) or multicolor GISH was performed according to previously published protocols (Kwiatek et al, 2016a,c). Mitotic chromosomes were identified using fluorescence in situ hybridization (FISH) with the repetitive sequences from pTa-86, pTa-103, and pTa-535 clones as well as GAA microsatellite characterized by Komuro et al (2013), amplified from genomic DNA of wheat (Chinese Spring) according to Kwiatek et al (2016b), and labeled by nick translation kits (Jena Bioscience) using Atto-488, Atto-550, and Atto-647 dyes.…”

Characterization of morphology and resistance to Blumeria graminis of winter triticale monosomic addition lines with chromosome 2D of Aegilops tauschii

“…This kind of genetic stocks is widely used for physical mapping of specific chromosomes (Kynast et al 2004 ) and analyzing alien genes expression (Cho et al 2006 ). The production of alien addition lines and the introgressions with alien chromosome segments carrying target traits can be induced by homoeologous recombination or the gametocidal effect (Kwiatek et al 2016a , b ).…”