TRANSMISSION OF TWO STRAINS OF EPIZOOTIC HEMORRHAGIC DISEASE VIRUS IN DEER BY Culicoides variipennis

Foster, N. M.; Breckon, R.; Luedke, A J; Jones, Robert H.; Metcalf, H. E.

doi:10.7589/0090-3558-13.1.9

Cited by 79 publications

(47 citation statements)

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“…In the US, C. variipennis var. sonorensis midges have been reported as the primary vector of EHDV and BTV (Foster et al, 1977;Tabachnick, 1996;Tabachnick, 2004). Apparently, very little information is available about the main vector (s) involved in the transmission of BTV and EHDV outside US in the American continent (Lager, 2004).…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) in captured Culicoides spp. in the northeastern regions of Mexico

n¹,

Balderas²,

Salas³

et al. 2015

Afr. J. Microbiol. Res.

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Section: Discussionmentioning

confidence: 99%

Molecular detection of bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) in captured Culicoides spp. in the northeastern regions of Mexico

n¹,

Balderas²,

Salas³

et al. 2015

Afr. J. Microbiol. Res.

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“…In North America, Culicoides sonorensis is suspected to be a main vector of bluetongue virus [1], [2] and epizootic hemorrhagic disease virus [3], [4], orbiviruses which cause hemorrhagic diseases and economic loss in ruminants such as sheep and deer. Both male and female biting midges are able to sustain themselves through sucrose feeding, which is usually acquired in the form of extrafloral nectar and serves as a quick energy source for flight [5].…”

Section: Introductionmentioning

confidence: 99%

The Reference Transcriptome of the Adult Female Biting Midge (Culicoides sonorensis) and Differential Gene Expression Profiling during Teneral, Blood, and Sucrose Feeding Conditions

2014

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Unlike other important vectors such as mosquitoes and sandflies, genetic and genomic tools for Culicoides biting midges are lacking, despite the fact that they vector a large number of arboviruses and other pathogens impacting humans and domestic animals world-wide. In North America, female Culicoides sonorensis midges are important vectors of bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV), orbiviruses that cause significant disease in livestock and wildlife. Libraries of tissue-specific transcripts expressed in response to feeding and oral orbivirus challenge in C. sonorensis have previously been reported, but extensive genome-wide expression profiling in the midge has not. Here, we successfully used deep sequencing technologies to construct the first adult female C. sonorensis reference transcriptome, and utilized genome-wide expression profiling to elucidate the genetic response to blood and sucrose feeding over time. The adult female midge unigene consists of 19,041 genes, of which less than 7% are differentially expressed during the course of a sucrose meal, while up to 52% of the genes respond significantly in blood-fed midges, indicating hematophagy induces complex physiological processes. Many genes that were differentially expressed during blood feeding were associated with digestion (e.g. proteases, lipases), hematophagy (e.g., salivary proteins), and vitellogenesis, revealing many major metabolic and biological factors underlying these critical processes. Additionally, key genes in the vitellogenesis pathway were identified, which provides the first glimpse into the molecular basis of anautogeny for C. sonorensis. This is the first extensive transcriptome for this genus, which will serve as a framework for future expression studies, RNAi, and provide a rich dataset contributing to the ultimate goal of informing a reference genome assembly and annotation. Moreover, this study will serve as a foundation for subsequent studies of genome-wide expression analyses during early orbivirus infection and dissecting the molecular mechanisms behind vector competence in midges.

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“…Both viral types can infect animals simultaneously (Prestwood et al, 1974;Thomas et al, 1974). Biting midges of the genus Culicoides are the principal vectors for EHDV and BTV (Foster et al, 1977;Tabachnick, 1996). In Arizona, few HD cases have been identified in freeranging wildlife, but antibody presence has been documented in cattle, deer, and pronghorn (Metcalf et al, 1981;Heffelfinger et al, 1999).…”

mentioning

confidence: 99%

Epizootiology of Hemorrhagic Disease in Mule Deer in Central Arizona

Dubay

Devos

Noon³

et al. 2004

Journal of Wildlife Diseases

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We report two male mule deer (Odocoileus hemionus) mortalities that occurred near Prescott, Arizona (USA) in September 2001. Necropsy lesions were compatible with those described for hemorrhagic disease (HD). Bluetongue (BTV) and epizootic hemorrhagic disease (EHDV) viral RNA were identified in tissues from one deer and EHDV viral RNA was identified in the other via reverse transcription-polymerase chain reaction (RT-PCR). Virus isolation attempts were unsuccessful in both cases. To determine geographic range and viral serotypes involved, we collected blood samples from healthy hunterharvested male mule deer in three game management units near Prescott in October 2001. Forty-one blood samples were collected and antibodies against EHDV and/or BTV were found in 21/41 (51%) samples as determined by agar immunodiffusion. Serum neutralization test results suggest that multiple EHDV and BTV serotypes are present in Arizona.

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TRANSMISSION OF TWO STRAINS OF EPIZOOTIC HEMORRHAGIC DISEASE VIRUS IN DEER BY Culicoides variipennis

Cited by 79 publications

References 0 publications

Molecular detection of bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) in captured Culicoides spp. in the northeastern regions of Mexico

Molecular detection of bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) in captured Culicoides spp. in the northeastern regions of Mexico

The Reference Transcriptome of the Adult Female Biting Midge (Culicoides sonorensis) and Differential Gene Expression Profiling during Teneral, Blood, and Sucrose Feeding Conditions

Epizootiology of Hemorrhagic Disease in Mule Deer in Central Arizona

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