Radio over fiber (RoF) techniques are good candidates to create the backbone of the next generation of wireless networks. Many parameters affect RoF communications such as amplified spontaneous emission noise (ASE), four-wave mixing nonlinearity (FWM), the modulation, channel spacing, switching voltage, and phase shifter. In this paper, we propose an improved model of RoF communication systems using subcarrier multiplexing/wavelength division multiplexing (SCM/WDM) technique with unequal channel spacing and 1-km Erbium-doped fiber amplifier (EDFA). Simulation results confirmed that we could obtain the lowest bit error rate and noises when the EDFA is placed at 1 km from the transmitter by using optical single-sideband (OSSB) modulation at frequencies 193.1 THz, 193.2 THz, 193.35 THz, and 193.6 THz.