Transmission-scanning electron microscopic observations of selected Eikenella corrodens strains

Progulske, A; Holt, Stanley C.

doi:10.1128/jb.143.2.1003-1018.1980

Cited by 24 publications

(10 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The surfaces of many of the bacteria stained with ruthenium red. This observation is consistent with numerous studies demonstrating the presence of ruthenium red-positive material on bacterial surfaces (Pate and Ordal, 1967;Springer and Roth, 1973;Fuller and Brooker, 1974;Brooker and Fuller, 1976;Kasper, 1976;Woo et al, 1979;Progulske and Holt, 1980;Costerton et al, 1981;Baker and Marcus, 1982). In most cases stain-ing is thought to be due to the presence of polysaccharide components in the bacterial glycocalyx (see review by Costerton et al, 1981).…”

Section: Epithelium Of the Cyclesupporting

confidence: 88%

Ruthenium red staining of vaginal epithelial cells and adherent bacteria

King

1985

Anat. Rec.

View full text Add to dashboard Cite

The vaginal epithelium of the rhesus monkey is a keratinized stratified squamous epithelium throughout the menstrual cycle and early pregnancy. The superficial cells have a thickened cell envelope, surface microridges, and numerous adherent bacteria. During later pregnancy the cells mucify and have a typical cell membrane, microvilli, and no adherent bacteria. In the present study we have extended these observations by examining vaginal surface structures after ruthenium red staining. Throughout the cycle, the superficial cells have a thin layer of stained material closely associated with the cell membrane, but in some cases a much thicker mucous blanket was observed. During later pregnancy the epithelial cells had a moderately thick, somewhat clumped ruthenium red-positive material associated with the cell membrane. Glycocalyx components of the surface of many bacteria also stained with ruthenium red. The adherence of many types of bacteria to the vaginal epithelial cells appears to be effected by the interaction of polyanionic components on the surface of both the bacterial and epithelial cells.

show abstract

Section: Epithelium Of the Cyclesupporting

confidence: 88%

Ruthenium red staining of vaginal epithelial cells and adherent bacteria

King

1985

Anat. Rec.

View full text Add to dashboard Cite

show abstract

“…Thus, EeLS were present on the outer surface of outer membrane. Certain strains of E.· corrodens have been reported to have fimbriae (20). However, we could not find fimbriae on E. corrodens 1073 in a previous study (21).…”

Section: Electron Microscopic Studycontrasting

confidence: 76%

“…Many bacteria have adhesin on their cell surf~tce appendages such as fimbriae or fibril (20). In a previous study, we could not find such appendages of the E. corrodens cell surface (21 ).…”

Section: Discussionmentioning

confidence: 77%

Production and characterization of monoclonal antibodies against bacterial lectin of Eikenella corrodens

Nakae¹,

Ebisu²,

Okada³

1993

J of Periodontal Research

View full text Add to dashboard Cite

A lectin-like substance (EeLS) was purified from the Eikenella corrodens 1073 cell and monoclonal antibodies were produced against it to confirm the role of EeLS in adhesive properties of E. corrodens such as hemagglutination and coaggregation with oral bacteria. Four hybridoma clones were selected. Two of the antibodies were of the IgG I isotype and the others were of the IgG2b isotype. These monoclonal antibodies inhibited both the hemagglutination of E. corrodens and the coaggregation with Actinomyces riscosus or Streptococcus sanguis. The reactivity of the monoclonal antibody to E. corrodens 1073 was significantly higher than that to E. corrodens 1080 of which adhesive activity was weaker than that of E. corrodens 1073. These findings suggest the difference in adhesive properties is due to the difference in the amount of EeLS expressed on the cell surface. The immunoelectron microscopic study revealed that EeLS of E. corrodens 1073 was localized in the outer space of outer membrane, not in cell surface appendages such as fimbriae where bacteria possessed adhesin. These results suggest that coaggregation of E. corrodens with A. viscosus or S. sanguis was mediated by EeLS. Copyright ·c Munksgaard IYYJ JOURNAL OF PERIODONTAL RESEARCH

show abstract

“…It has been used in routine histochemical staining (Pearse, 1985) to demonstrate acid mucins, and in electron microscopy to preserve carbohydrate containing cellular coats for mammalian (Behnke and Zelander, 1970) and prokaryotic cells (Progulaske and Holt, 1980). For some authors, results using AB have agreed with those obtained by RR for some workers (Behnke and Zelander, 1970) but for others, staining between strains of the same bacterial species have differed (Progulske and Holt, 1980).…”

Section: Introductionsupporting

confidence: 74%

“…Many EM preparative procedures fail to optimally preserve components of the bacterial envelope, either by loss or collapse during dehydration stages (Costerton, 1980; Molinari et al, 1988;Roth, 1977) or insufficent stabilization during fixation (Progulske and Holt, 1980). Attempts to preserve and stabilize these delicate layers have included use of specific lectins (Molinari et al, 19881, specific antibodies alone (Molinari et al, 1987;Progulske and Holt, 1980) or in combination with ruthenium red (RR) (Mackie et al, 1979;Patterson et al, 19751, RR alone (Fassel et al, 1990;Luft, 1971aLuft, , 1971b; Progulske and Holt, 1980;Titus et al, 1982) and alcian blue (AB) (Progulske and Holt, 1980). Additional approaches exist, such as those utilizing high iron diamine, dialyzed colloidal iron, and colloidal thorium (Spicer et al, 1978;Thomopoulos et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Alcian blue and ruthenium red effects on preservation of outer envelope ultrastructure in methanotrophic bacteria

Fassel

Schaller

Remsen

1992

Microscopy Res & Technique

View full text Add to dashboard Cite

Alcian blue (AB) and ruthenium red (RR) effects on ultrastructural preservation of the bacterial cell envelope of methanotrophs are compared. A previous successful method with RR that enhanced preservation of outer envelope layers in two representative methanotroph species is applied to other genera and species of methanotropic bacteria. Alcian blue is substituted for RR in this en bloc protocol. The effect of AB on preservation of these layers is assessed at the ultrastructural level and compared to RR for all species examined. Further, comparison with freeze etch and a fixation in the absence of either RR or AB is made. Both RR and AB are found to aid preservation and help visualize additional components of the cell envelope which are lost or minimized in a standard fixation not employing these cationic reagents. For some species, images obtained are similar between RR and AB procedures and agree with images seen by freeze etch. For other species, AB preserves extended filamentous material that is partially condensed even with the use of RR. Thus, use of AB improves the preservation of outer envelope structure in these organisms equally or more effectively than use of RR.

show abstract

Transmission-scanning electron microscopic observations of selected Eikenella corrodens strains

Cited by 24 publications

References 36 publications

Ruthenium red staining of vaginal epithelial cells and adherent bacteria

Ruthenium red staining of vaginal epithelial cells and adherent bacteria

Production and characterization of monoclonal antibodies against bacterial lectin of Eikenella corrodens

Comparison of Alcian blue and ruthenium red effects on preservation of outer envelope ultrastructure in methanotrophic bacteria

Contact Info

Product

Resources

About