The computational fluid dynamics (CFD) simulation on the butte was carried out to obtain the wind field characteristics for a specific mountain in the hilly area of eastern China. Then, the speed-up ratios of butte at each location distributed by height were calculated. The simulation results were compared with the specified value of mountain wind field acceleration ratio in various codes. The finite element model of the transmission tower line system is established by ANSYS, and the wind-induced vibration response of the structural system under the single mountain wind field is calculated, which is compared with the results of flat ground. The results show that the mountain will hinder the fluid passing through it. There is a deceleration zone in front of the mountain and a flow separation phenomenon behind the mountain. The speed-up ratios at the butte top where accelerating effect are the biggest among all positions of the butte. As the height increases, they approach 1. The speed-up ratios calculated by the Chinese code and the Australian code are linearly changing along the ridge of the butte and are symmetric at the windward side and leeward side. The biggest speed-up ratios are calculated by the Chinese code, and the smallest ratios are calculated by CFD. The wind-induced vibration response of the transmission tower line system is influenced by the wind speed-up ratios and reaches the maximum at the top of the butte. The simulation results at the windward side are close to the values calculated by the Australia code and the Euro code, but at the top and leeward side, they are far smaller than others.