Transplantation of cardiac-committed mouse embryonic stem cells to infarcted sheep myocardium: a preclinical study

In vitro cardiomyogenic differentiation of adipose‐derived stromal cells using transforming growth factor‐β1

“…Bone morphogenic protein-2 also up-regulated cardiac gene expression in mouse embryonic stem cells. 20 In this study, we investigated whether TGF-b1 induces ADSC cardiomyogenic differentiation in vitro. The TGF-b1 stimulatory effects on ADSC cardiogenic differentiation were evaluated by immunohistochemistry, flow cytometric analyses, and reverse transcription-polymerase chain reaction (RT-PCR).…”

A lentiviral vector with a short troponin-I promoter for tracking cardiomyocyte differentiation of human embryonic stem cells

“…15 In addition, several reports demonstrated that mESC-derived CMCs can integrate with host myocardium, generating electromechanical coupling capable of substituting the loss in myocardial contractile force; 16 more recently, similar therapeutic effects were demonstrated using mouse ESC-derived CMCs in a sheep model of heart failure. 17 While bulk cultures of ESCderived differentiated CMCs can be obtained from mouse ESCs using transfection of plasmids with tissuespecific promoters and marker selection, 16 …”

Cardiac Repair Using Stem Cells Conditioned Media Based Therapy in a Rodent Model of Myocardial Infarction and Heart Failure

“…human and animal models, to varying degrees of success [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] . In animal models, these cellbased therapies have demonstrated effectiveness in reducing infarct size and ventricular dilatation, and improving myocardial function [24][25][26] . Similar results have been demonstrated in human models, however clinical translation is limited 8,13,19 .…”