Transplantation of human amnion prevents recurring adhesions and ameliorates fibrosis in a rat model of sciatic nerve scarring

Lemke, Angela; Ferguson, James; Groß, Kelly; Penzenstadler, Carina; Bradl, Monika; Mayer, Rupert L.; Gerner, Christopher; Redl, Heinz; Wolbank, Susanne

doi:10.1016/j.actbio.2017.11.042

Cited by 40 publications

(43 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Swing Time was significantly increased after femoral nerve resection, whereas Swing Speed was decreased significantly, so their product remained constant. The same phenomenon was observable in rats after sciatic nerve neurotmesis (Bozkurt et al, ; Deumens et al, ) or induction of sciatic nerve fibrosis (Lemke et al, ). An explanation for these findings may be the rats' trait to keep paw placement of both ipsilateral paws on each side almost completely superimposed while proceeding forward (Hruska, Kennedy, & Silbergeld, ).…”

Section: Discussionmentioning

confidence: 58%

“…Maintenance of identical Stride Length indicates a supporting mechanism in the case of altered or impaired hind limb function by trying to maintain balance and secure footing during locomotion. Accordingly, Stride Length is altered after central nervous injury and unchanged after peripheral nerve injury in rats (Bozkurt, Deumens, et al, ; Hamers et al, , ; Lemke et al, ). BoS was slightly elevated after surgery and showed only minor variation throughout the entire observation period.…”

Section: Discussionmentioning

confidence: 99%

“…Among these are the high incidence of limb contractures and automutilation as well as the onset of neuropathic pain (Irintchev, ; Tos et al, ). Additionally, the severity of the nerve injury plays a major role in assessment of functional recovery after sciatic nerve injury, since full recovery to baseline seems only to occur in models of crush injury (axonotmesis) and, as recently proven, in models of epineural fibrosis (Bozkurt, Deumens, et al, ; Bozkurt et al, ; Lemke et al, ). In contrast, most of the parameters of gait merely reached around 50% of baseline 10 weeks after sciatic nerve resection and autograft repair (Bozkurt et al, ; Deumens et al, ).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

The course of recovery of locomotor function over a 10‐week observation period in a rat model of femoral nerve resection and autograft repair

2020

Self Cite

View full text Add to dashboard Cite

Background:A great extent of knowledge on peripheral nerve regeneration has been gathered using the rat sciatic nerve model. The femoral nerve model of the rat offers an interesting alternative, as it lacks disadvantageous features such as automutilation. For the analysis of locomotor behavior in rats after sciatic nerve injury, the CatWalk ™ XT Gait Analysis System is often used. However, lesions of the femoral nerve in the rat have yet remained unstudied with this method. Material and Methods: Ten male Sprague Dawley rats were evaluated with theCatWalk XT to study their gait after a 6-mm resection of the right femoral nerve and reconstruction with an autologous nerve graft. Animals were observed for 10 weeks after surgery.Results: Print Area, Print Length, Swing Speed, and Duty Cycle decreased to a minimum of 40% of baseline 2 weeks after surgery. Swing Time was elevated more than twofold at this time point. However, all these parameters recovered back to >90% of baseline values at 10 weeks after surgery. This degree of functional recovery has not been reported after sciatic nerve resection and autograft repair. Base of support varied minimally postoperatively in contrast to a strong decrement after sciatic nerve resection and repair. Conclusion:We hereby provide a comprehensive in-depth analysis of how to study functional recovery after injury of the femoral nerve in the rat via the CatWalk XT.We place special emphasis on highlighting the differences between the femoral nerve and sciatic nerve injury model in this context. K E Y W O R D SCatWalk XT, femoral nerve, functional recovery, gait analysis, rats, sciatic nerve

show abstract

Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The course of recovery of locomotor function over a 10‐week observation period in a rat model of femoral nerve resection and autograft repair

2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…They are available, flexible, robust, and offer unique anti-inflammatory and anti-fibrotic properties that allow optimal regeneration [12]. HAM contains cytokines, growth factors, and neurotrophic factors that slow down the inflammatory response [13,14]. Mesenchymal amniotic cells have stem cell-like properties [15].…”

Section: Introductionmentioning

confidence: 99%

“…Amnion is thus a promising biomaterial for regenerative medicine and is excellently suited for use in the urinary tract, as shown in our own experimental and clinical work [18][19][20][21]. New data show the prevention of nerve fibrosis by amniotic wounds in animal experiments [14]. However, the current level of evidence is low because of the few experimental and retrospective studies available.…”

Section: Introductionmentioning

confidence: 99%

Application of Dried Human Amnion Graft to Improve Post-Prostatectomy Incontinence and Potency: A Randomized Exploration Study Protocol

et al. 2019

View full text Add to dashboard Cite

Introduction: Incontinence (up to 20%) and erectile dysfunction (up to 70%) occur frequently after radical prostatectomy (RP) in patients with localized prostate cancer. Human amniotic membrane (HAM) can improve tissue regeneration and functional outcome after RP owing to the growth factors and unique immune tolerance. Preliminary studies showed the potential value of HAM in the reconstruction of the urinary tract and nerve protection during RP. Methods: A protocol is developed for a prospective, randomized, single-blind, singlesurgeon, placebo-controlled exploration study of the efficacy and safety of dehydrated human amnion membrane placed around the neurovascular bundle (NVB) and vesicourethral anastomosis (VUA) during RP for the treatment of localized prostate cancer. Eligible for inclusion are patients with localized prostate cancer, requiring a surgical procedure and exclusion of preoperative incontinence and erectile dysfunction. The patients are randomized 1:1 to HAM vs. placebo and blinded during the study Enhanced Digital Features To view enhanced digital features for this article go to https://doi.org/10.6084/ m9.figshare.10283555.

show abstract

Assessment of fresh and preserved amniotic membrane for guided bone regeneration in mice

Fénelon

Etchebarne

Siadous

et al. 2020

J Biomedical Materials Res

View full text Add to dashboard Cite

Thanks to its biological properties, the human amniotic membrane (HAM) can be used as a barrier membrane for guided bone regeneration (GBR). However, no study has assessed the influence of the preservation method of HAM for this application. This study aimed to establish the most suitable preservation method of HAM for GBR. Fresh (F), cryopreserved (C) lyophilized (L), and decellularized and lyophilized (DL) HAM were compared. The impact of preservation methods on collagen and glycosaminoglycans (GAG) content was evaluated using Masson's trichrome and alcian blue staining. Their suture retention strengths were assessed. In vitro, the osteogenic potential of human bone marrow mesenchymal stromal cells (hBMSCs) cultured on the four HAMs was evaluated using alkaline phosphatase staining and alizarin red quantification assay. In vivo, the effectiveness of fresh and preserved HAMs for GBR was assessed in a mice diaphyseal bone defect after 1 week or 1 month healing. Micro‐CT and histomorphometric analysis were performed. The major structural components of HAM (collagen and GAG) were preserved whatever the preservation method used. The tearing strength of DL‐HAM was significantly higher. In vitro, hBMSCs seeded on DL‐HAM displayed a stronger ALP staining, and alizarin red staining quantification was significantly higher at Day 14. In vivo, L‐HAM and DL‐HAM significantly enhanced early bone regeneration. One month after the surgery, only DL‐HAM slightly promoted bone regeneration. Several preserving methods of HAM have been studied for bone regeneration. Here, we have demonstrated that DL‐HAM achieved the most promising results for GBR.

show abstract

Transplantation of human amnion prevents recurring adhesions and ameliorates fibrosis in a rat model of sciatic nerve scarring

Cited by 40 publications

References 75 publications

The course of recovery of locomotor function over a 10‐week observation period in a rat model of femoral nerve resection and autograft repair

The course of recovery of locomotor function over a 10‐week observation period in a rat model of femoral nerve resection and autograft repair

Application of Dried Human Amnion Graft to Improve Post-Prostatectomy Incontinence and Potency: A Randomized Exploration Study Protocol

Assessment of fresh and preserved amniotic membrane for guided bone regeneration in mice

Contact Info

Product

Resources

About