Transplantation of human dental pulp stem cells in streptozotocin-induced diabetic rats

El-Kersh, Ahmed Othman Fathy Othman; El–Akabawy, Gehan; Al-Serwi, Rasha Hamed

doi:10.1007/s12565-020-00550-2

Cited by 12 publications

(19 citation statements)

References 70 publications

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“…9 Human DPSCs are reported to have a capability to contribute to the regeneration of a large array of tissues ranging from oral and maxillofacial defects, 10 to corneal disease, 11 and pancreatic islet. 12 Despite remarkable progress toward the biomedical application of stem cells, 13,14 isolation, expansion, and differentiation of MSCs under clinical conditions are still challenging. 15 Conventional regenerative procedures are typically associated with the use of biological components that causes serious concerns for translating basic scientific reports from the bench to patient care.…”

Section: Introductionmentioning

confidence: 99%

<p>ZIF-8 Modified Polypropylene Membrane: A Biomimetic Cell Culture Platform with a View to the Improvement of Guided Bone Regeneration</p>

Ejeian¹,

Razmjou²,

Nasr-Esfahani³

et al. 2020

IJN

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Purpose Despite the significant advances in modeling of biomechanical aspects of cell microenvironment, it remains a major challenge to precisely mimic the physiological condition of the particular cell niche. Here, the metal–organic frameworks (MOFs) have been introduced as a feasible platform for multifactorial control of cell-substrate interaction, given the wide range of physical and mechanical properties of MOF materials and their structural flexibility. Results In situ crystallization of zeolitic imidazolate framework-8 (ZIF-8) on the polydopamine (PDA)-modified membrane significantly raised surface energy, wettability, roughness, and stiffness of the substrate. This modulation led to an almost twofold increment in the primary attachment of dental pulp stem cells (DPSCs) compare to conventional plastic culture dishes. The findings indicate that polypropylene (PP) membrane modified by PDA/ZIF-8 coating effectively supports the growth and proliferation of DPSCs at a substantial rate. Further analysis also displayed the exaggerated multilineage differentiation of DPSCs with amplified level of autocrine cell fate determination signals, like BSP1, BMP2, PPARG, FABP4, ACAN , and COL2A . Notably, osteogenic markers were dramatically overexpressed (more than 100-folds rather than tissue culture plate) in response to biomechanical characteristics of the ZIF-8 layer. Conclusion Hence, surface modification of cell culture platforms with MOF nanostructures proposed as a powerful nanomedical approach for selectively guiding stem cells for tissue regeneration. In particular, PP/PDA/ZIF-8 membrane presented ideal characteristics for using as a barrier membrane for guided bone regeneration (GBR) in periodontal tissue engineering.

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Section: Introductionmentioning

confidence: 99%

<p>ZIF-8 Modified Polypropylene Membrane: A Biomimetic Cell Culture Platform with a View to the Improvement of Guided Bone Regeneration</p>

Ejeian¹,

Razmjou²,

Nasr-Esfahani³

et al. 2020

IJN

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“…In addition, glucose is the main source of ROS production [ 54 ]. Our previous study demonstrated that hDPSCs can migrate to and survive within the STZ-injured pancreas and subsequently exert antidiabetic effects by differentiating into β-cells and inducing paracrine-mediated pancreatic regeneration [ 55 ]. Therefore, available scientific literature suggests that glycaemic control may play a role in hindering oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Human dental pulp stem cells attenuate streptozotocin-induced parotid gland injury in rats

2021

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Objective Diabetes mellitus causes deterioration in the body, including serious damage of the oral cavity related to salivary gland dysfunction, characterised by hyposalivation and xerostomia. Human dental pulp stem cells (hDPSCs) represent a promising therapy source, due to the easy, minimally invasive surgical access to these cells and their high proliferative capacity. It was previously reported that the trophic support mediated by these cells can rescue the functional and structural alterations of damaged salivary glands. However, potential differentiation and paracrine effects of hDPSCs in diabetic-induced parotid gland damage have not been investigated. Our study aimed to investigate the therapeutic effects of intravenous transplantation of hDPSCs on parotid gland injury in a rat model of streptozotocin (STZ)-induced type 1 diabetes. Methods Thirty Sprague–Dawley male rats were randomly categorised into three groups: control, diabetic (STZ), and transplanted (STZ + hDPSCs). The hDPSCs or the vehicles were injected into the rats’ tail veins, 7 days after STZ injection. Fasting blood glucose levels were monitored weekly. A glucose tolerance test was performed, and the parotid gland weight, salivary flow rate, oxidative stress indices, parotid gland histology, and caspase-3, vascular endothelial growth factor, proliferating cell nuclear antigen, neuronal nitric oxide synthase, endothelial nitric oxide synthase, and tetrahydrobiopterin biosynthetic enzyme expression levels in parotid tissues were assessed 28 days post-transplantation. Results Transplantation of hDPSCs decreased blood glucose, improved parotid gland weight and salivary flow rate, and reduced oxidative stress. The cells migrated to the STZ-injured parotid gland and differentiated into acinar, ductal, and myoepithelial cells. Moreover, hDPSCs downregulated the expression of caspase-3 and upregulated the expression of vascular endothelial growth factor and proliferating cell nuclear antigen, likely exerting pro-angiogenic and anti-apoptotic effects and promoting endogenous regeneration. In addition, the transplanted cells enhanced the parotid nitric oxide-tetrahydrobiopterin pathway. Conclusions Our results showed that hDPSCs migrated to and survived within the STZ-injured parotid gland, where functional and morphological damage was prevented due to the restoration of normal glucose levels, differentiation into parotid cell populations, and stimulation of paracrine-mediated regeneration. Thus, hDPSCs may have potential in the treatment of diabetes-induced parotid gland injury.

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“…In the treatment of these diseases using DPSCs, injection is one of the more commonly used forms. Stem cell injections are the easiest cell therapy products to prepare, and the commonly used vehicles at present for resuspension of stem cells is phosphate-buffered saline[ 69 - 73 ], Hank's Balanced Salt Solution[ 15 ], normal saline[ 14 , 67 , 74 - 76 ], and modified medium[ 66 , 68 , 77 - 79 ]. These mediators have no therapeutic effect and only serve as transport vectors for stem cells, so it is possible to observe the monotherapy effect of transplanted stem cells and rule out the influence of carriers.…”

Section: Derivatives Of Dpscsmentioning

confidence: 99%

“…Both intravenously and locally injected DPSCs have been shown to improve symptoms of various lesions[ 15 , 72 , 76 , 80 ]. DPSCs have also been shown to migrate and integrate into the site of injuries[ 15 , 69 , 73 , 74 ] and differentiate into corresponding cells[ 68 , 72 , 75 , 79 , 84 ].…”

Section: Derivatives Of Dpscsmentioning

confidence: 99%

Therapeutic potential of dental pulp stem cells and their derivatives: Insights from basic research toward clinical applications

Yuan¹,

Yang²,

Zhang³

et al. 2022

WJSC

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For more than 20 years, researchers have isolated and identified postnatal dental pulp stem cells (DPSCs) from different teeth, including natal teeth, exfoliated deciduous teeth, healthy teeth, and diseased teeth. Their mesenchymal stem cell (MSC)-like immunophenotypic characteristics, high proliferation rate, potential for multidirectional differentiation and biological features were demonstrated to be superior to those of bone marrow MSCs. In addition, several main application forms of DPSCs and their derivatives have been investigated, including stem cell injections, modified stem cells, stem cell sheets and stem cell spheroids. In vitro and in vivo administration of DPSCs and their derivatives exhibited beneficial effects in various disease models of different tissues and organs. Therefore, DPSCs and their derivatives are regarded as excellent candidates for stem cell-based tissue regeneration. In this review, we aim to provide an overview of the potential application of DPSCs and their derivatives in the field of regenerative medicine. We describe the similarities and differences of DPSCs isolated from donors of different ages and health conditions. The methodologies for therapeutic administration of DPSCs and their derivatives are introduced, including single injections and the transplantation of the cells with a support, as cell sheets, or as cell spheroids. We also summarize the underlying mechanisms of the regenerative potential of DPSCs.

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Transplantation of human dental pulp stem cells in streptozotocin-induced diabetic rats

Cited by 12 publications

References 70 publications

<p>ZIF-8 Modified Polypropylene Membrane: A Biomimetic Cell Culture Platform with a View to the Improvement of Guided Bone Regeneration</p>

<p>ZIF-8 Modified Polypropylene Membrane: A Biomimetic Cell Culture Platform with a View to the Improvement of Guided Bone Regeneration</p>

Human dental pulp stem cells attenuate streptozotocin-induced parotid gland injury in rats

Therapeutic potential of dental pulp stem cells and their derivatives: Insights from basic research toward clinical applications

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