Transplantation of human meningioma stem cells loaded on a self-assembling peptide nanoscaffold containing IKVAV improves traumatic brain injury in rats

Negah, Sajad Sahab; Oliazadeh, Pardis; Jahan-Abad, Ali Jahanbazi; Eshaghabadi, Arezou; Samini, Fariborz; Ghasemi, Sepideh; Asghari, Amir; Gorji, Ali

doi:10.1016/j.actbio.2019.05.010

Cited by 45 publications

(33 citation statements)

References 56 publications

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“…[ 9a,64 ] Furthermore, designer self‐assembling peptide scaffolds may be designed to realize extensive biological functionality of hydrogel matrices, such as the desired physicochemical properties, desirable mechanical stiffness and possible biological cues for cell growth in vitro, including customizing the inherent native interactions of cells with ECM, and its consequent in situ microtissue remodeling. [ 9b,64,65 ] So, choosing designer self‐assembling peptide hydrogel to culture cells for 3D tissue remodeling in vitro, it is possible to better emulate the physiology of their original ECM in specific tissue types. Compared with various natural hydrogels and synthetic polymer hydrogels (Table 1), in designer self‐assembling peptide hydrogels, dipeptides, tripeptides, tetrapeptide, and their many times repeated peptide sequences are exciting hierarchical main building blocks for various subset of hydrogels, which surely represent biological or inspired candidate motifs to realize molecular bioengineering assembly strategy for precise 3D tissue reconstructs in vitro.…”

Section: Common Hydrogel Products and Biomedical Featuresmentioning

confidence: 99%

“…Designer self‐assembling peptide hydrogels span over past three decades from serendipitous discovery of the first self‐assembling peptide EAK16‐II in 1990 to a large range of biomedical applications, including cell or drug and antibody carriers, [ 66 ] stem cell scaffolds, [ 9a,67 ] microtissue formation in vitro, [ 65 ] and novel peptide detergents or surfactants. [ 23c ] The other self‐assembling peptide elements have emerged by a similar molecular design strategy in the past decade years ( Figure ), mainly including glutamine‐rich peptides, β‐hairpin peptides, α‐helix peptides, coiled‐coil peptides, multidomain peptide, and aromatic short peptide derivatives (partially indicated in Table 2 ).…”

Section: Common Hydrogel Products and Biomedical Featuresmentioning

confidence: 99%

“…In designer self‐assembling peptide hydrogels, the canonical amino acid residues serve as basic molecular building blocks of nanofiber scaffold networks, which confer inherent biocompatibility, high hydrophilicity, chemical amenability, and biodegradation in vivo. Because of these prominent advantages, designer self‐assembling peptide hydrogels are proposed to fabricate various 3D ex vivo microtissue models, [ 65 ] tumor organoids for preclinical drug discovery, [ 80 ] 3D cell culture constructs for clinical drug repositioning, [ 3 ] therapeutic drug or cell delivery carrier, [ 66b,81 ] and new generation self‐adjuvant vaccine design. [ 82 ] Over the past decades, extensive biomedical researches and translational and clinical trials greatly enlarged our understanding of basic cancer research.…”

Section: Common Hydrogel Products and Biomedical Featuresmentioning

confidence: 99%

“…Schematic cell constructs are recently developed for tissue‐specific modeling, which is available or served as already tested tissue remodeling strategies in designer self‐assembling peptide hydrogels. A) Human stem cell‐based cell construct provides alternative options for peripheral nerve regeneration and repair strategies in RADA16‐I hydrogel, [ 65 ] in addition to the cell construct in EFK‐I hydrogel, which is effectively injectable cell delivery platform for intervertebral disc repair applications. [ 66b ] B) P11 peptide hydrogel directly indicates in situ clinical treatment benefit for early buccal carious lesions.…”

Section: Instructive Cell Constructs In Tissue Engineering and Precismentioning

confidence: 99%

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Designer Self‐Assembling Peptide Hydrogels to Engineer 3D Cell Microenvironments for Cell Constructs Formation and Precise Oncology Remodeling in Ovarian Cancer

Yang

Zhao

2020

Advanced Science

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mouse intestinal stem cells and primary colorectal cancer cells can be propagated in vitro long term, [1] there is an urgent need to develop more physiologically relevant, efficient, and robust precise oncology models that closely recapitulate the genetic and morphological heterogeneous composition and mimic the arrangement pattern of cancer cells in the original tumor. [2] To our knowledge in biomedical research, hydrogel is the best tool to reconstruct precise oncology models in vitro, especially tumor organoid, which not only recapitulates in vivo biology and microenvironmental factors, but also at large extent allows side-by-side comparison to evaluate the translational potential of 3D model systems to the patients. [2a,3] Generally, hydrogels are mainly composed of hydrophilic polymeric scaffolds that absorb large amounts of water, so the hydrogel matrix better mimics elastic and viscoelastic properties in ECM and microscale topographies of cell matrices, which controls proper cell morphology, directs viable cell behaviors, and drives in vivo fundamental cell-cell or cell-ECM interactions. [4] To recapitulate pathophysiological features of human tumors and imitate various aspects of human tumorigenesis in vivo, hydrogels can provide a realistic platform to establish a useful bridge between in vitro assays and in vivo cell microenvironments. In current biomedical applications, there are many kinds of hydrogels to be developed to mimic the biological properties of ECM, such Designer self-assembling peptides form the entangled nanofiber networks in hydrogels by ionic-complementary self-assembly. This type of hydrogel has realistic biological and physiochemical properties to serve as biomimetic extracellular matrix (ECM) for biomedical applications. The advantages and benefits are distinct from natural hydrogels and other synthetic or semisynthetic hydrogels. Designer peptides provide diverse alternatives of main building blocks to form various functional nanostructures. The entangled nanofiber networks permit essential compositional complexity and heterogeneity of engineering cell microenvironments in comparison with other hydrogels, which may reconstruct the tumor microenvironments (TMEs) in 3D cell cultures and tissue-specific modeling in vitro. Either ovarian cancer progression or recurrence and relapse are involved in the multifaceted TMEs in addition to mesothelial cells, fibroblasts, endothelial cells, pericytes, immune cells, adipocytes, and the ECM. Based on the progress in common hydrogel products, this work focuses on the diverse designer self-assembling peptide hydrogels for instructive cell constructs in tissue-specific modeling and the precise oncology remodeling for ovarian cancer, which are issued by several research aspects in a 3D context. The advantages and significance of designer peptide hydrogels are discussed, and some common approaches and coming challenges are also addressed in current complex tumor diseases.

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Section: Common Hydrogel Products and Biomedical Featuresmentioning

confidence: 99%

Section: Common Hydrogel Products and Biomedical Featuresmentioning

confidence: 99%

Section: Common Hydrogel Products and Biomedical Featuresmentioning

confidence: 99%

Section: Instructive Cell Constructs In Tissue Engineering and Precismentioning

confidence: 99%

See 2 more Smart Citations

Designer Self‐Assembling Peptide Hydrogels to Engineer 3D Cell Microenvironments for Cell Constructs Formation and Precise Oncology Remodeling in Ovarian Cancer

Yang

Zhao

2020

Advanced Science

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“…Since this reaction takes place in situ, at physiological conditions (pH 7.4), relatively fast, and without the need of external stimuli (such as temperature or ionic strength), it could be useful as filler in traumatic brain injury (TBI) [42,43], acute spinal cord injury (SCI) [41,[44][45][46], or as sprayable hemostatic solution in combination with common clotting bandages during uncontrolled bleeding in surgeries [47].…”

Section: Edc/sulfo-nhs Cross-linked Peptide Preparationmentioning

confidence: 99%

Cross-Linked Self-Assembling Peptides and Their Post-Assembly Functionalization via One-Pot and In Situ Gelation System

Pugliese

Gelain

2020

IJMS

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Supramolecular nanostructures formed through peptide self-assembly can have a wide range of applications in the biomedical landscape. However, they often lose biomechanical properties at low mechanical stress due to the non-covalent interactions working in the self-assembling process. Herein, we report the design of cross-linked self-assembling peptide hydrogels using a one-pot in situ gelation system, based on 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide/N-hydroxysulfosuccinimide (EDC/sulfo–NHS) coupling, to tune its biomechanics. EDC/sulfo–NHS coupling led to limited changes in storage modulus (from 0.9 to 2 kPa), but it significantly increased both the strain (from 6% to 60%) and failure stress (from 19 to 35 Pa) of peptide hydrogel without impairing the spontaneous formation of β-sheet-containing nano-filaments. Furthermore, EDC/sulfo–NHS cross-linking bestowed self-healing and thixotropic properties to the peptide hydrogel. Lastly, we demonstrated that this strategy can be used to incorporate bioactive functional motifs after self-assembly on pre-formed nanostructures by functionalizing an Ac-LDLKLDLKLDLK-CONH2 (LDLK12) self-assembling peptide with the phage display-derived KLPGWSG peptide involved in the modulation of neural stem cell proliferation and differentiation. The incorporation of a functional motif did not alter the peptide’s secondary structure and its mechanical properties. The work reported here offers new tools to both fine tune the mechanical properties of and tailor the biomimetic properties of self-assembling peptide hydrogels while retaining their nanostructures, which is useful for tissue engineering and regenerative medicine applications.

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Advanced Materials to Enhance Central Nervous System Tissue Modeling and Cell Therapy

Muckom

Sampayo

Johnson

et al. 2020

Adv Funct Materials

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The progressively deeper understanding of mechanisms underlying stem cell fate decisions has enabled parallel advances in basic biology-such as the generation of organoid models that can further one's basic understanding of human development and disease-and in clinical translation-including stem cell based therapies to treat human disease. Both of these applications rely on tight control of the stem cell microenvironment to properly modulate cell fate, and materials that can be engineered to interface with cells in a controlled and tunable manner have therefore emerged as valuable tools for guiding stem cell growth and differentiation. With a focus on the central nervous system (CNS), a broad range of material solutions that have been engineered to overcome various hurdles in constructing advanced organoid models and developing effective stem cell therapeutics is reviewed. Finally, regulatory aspects of combined material-cell approaches for CNS therapies are considered.

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Transplantation of human meningioma stem cells loaded on a self-assembling peptide nanoscaffold containing IKVAV improves traumatic brain injury in rats

Cited by 45 publications

References 56 publications

Designer Self‐Assembling Peptide Hydrogels to Engineer 3D Cell Microenvironments for Cell Constructs Formation and Precise Oncology Remodeling in Ovarian Cancer

Designer Self‐Assembling Peptide Hydrogels to Engineer 3D Cell Microenvironments for Cell Constructs Formation and Precise Oncology Remodeling in Ovarian Cancer

Cross-Linked Self-Assembling Peptides and Their Post-Assembly Functionalization via One-Pot and In Situ Gelation System

Advanced Materials to Enhance Central Nervous System Tissue Modeling and Cell Therapy

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